1887

Abstract

Summary

Two cell lines of human T lymphocytes (H9 and CEM) chronically infected with isolates of human or simian immunodeficiency viruses were examined by electron microscopy. Scanning electron microscopy of H9 cells showed characteristic morphological changes in the cells after infection with human T cell lymphotropic virus type III (HTLV-III); these included loss of cell microvilli and their replacement by rounded surface protrusions. Virus particles were present over the whole cell surface, but were more numerous between, rather than on, surface protrusions. In contrast, CEM cells infected with three other isolates of the virus showed little change in morphology compared with uninfected cells; they typically had a single large aggregate of virus particles at the posterior end of the cell. Transmission electron microscopy of sections of infected cells showed budding virus in only a small proportion of cells although many had mature virus particles on their surface. The immature particles released by budding had a ring-like morphology in contrast to the mature virus with its characteristic elongated nucleoid. Surface spikes were rarely seen on sectioned virus particles, but were more obvious on the simian immunodeficiency virus than on human isolates. Negative staining of purified virus preparations showed that the peripheral dense material of immature particles had a striated structure. Surface spikes were not seen by negative staining on either immature or mature virus particles. A third type of smaller particle with surface spikes was found in all negatively stained preparations.

Keyword(s): electron microscopy , HIV and lymphocytes
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1988-10-01
2022-01-21
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References

  1. BAIGENT C. L., MANNWEILER K., ANDERSEN I., RUTTER G. A., NEUMAYER U. 1978; Verbessertes und vereinfachtes “austauschverfahren” zur präparation von infektiösen deckglaszell-kulturen für vergleichende REM und TEm-studien. Beiträge zur elektronenmikroskopischen Direktabbildung von Oberflachen 11:251–263
    [Google Scholar]
  2. BARRÉ-SINOUSSI F., CHERMANN I. C, REY F., NUGEYRE M. T., CHAMARET S., GRUEST J., DAUGUET C, AXLER-BLIN C, VEZINET-BRUN F., ROUZIOUX C, ROZENBAUM W., MONTAGNIER L. 1983; Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science 220:868–871
    [Google Scholar]
  3. BIBERFELD G., BROWN F., ESPARZA J., ESSEX M., GALLO R. C, MONTAGNIER L., NAJERA R., RISSER R., SCHILD G. C. 1987; Meeting report of WHO working group on characterization of HIV-related retroviruses: criteria for characterization and proposal for a nomenclature system. AIDS Research and Human Retroviruses 3:241–243
    [Google Scholar]
  4. BONAR R. A., HEINE U., BEARD D., BEARD I. W. 1963; Virus of a myeloblastosis (BAI strain A), XXIII. Morphology of virus and comparison with strain R (erythroblastosis). Journal of the National Cancer Institute 30:949–977
    [Google Scholar]
  5. BRETSCHER M. S. 1984; Endocytosis: relation to capping and cell locomotion. Science 224:681–686
    [Google Scholar]
  6. DUBOIS-DALCQ M., HOLMES K. V., RENTIER B. 1984 Assembly of Enveloped RNA Viruses Wein & New York: Springer-Verlag;
    [Google Scholar]
  7. FOLEY G. E., LAZARUS H., FABER S., UZMAN B. G., BOONE B. A., MCCARTHY R. E. 1965; Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18:522–529
    [Google Scholar]
  8. GALLO R. C, SALAHUDDIN S. Z., POPOVIC M., SHEARER G. M., KAPLAN M., HAYNES B. F., PALKER T. J., REDFIELD R., OLESKE J., SAFAL B., WHITE G., FOSTER P., MARKHAM P. D. 1984; Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science 224:500–503
    [Google Scholar]
  9. GELDERBLOM H., BAUER H., OGURA H., WIGAND R., FISCHER A. 1974; Detection of oncornavirus-like particles in HeLa cells. I. Fine structure and comparative morphological classification. International Journal of Cancer 13:246–253
    [Google Scholar]
  10. GELDERBLOM H. R., HAUSMANN E. H. S., OZEL M., PAULI G., KOCH M. A. 1987; Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins. Virology 156:171–176
    [Google Scholar]
  11. LECATSAS G., GRAVELL M., SEVER J. L. 1984; Morphology of the retroviruses associated with AIDS and SAIDS. Proceedings of the Society for Experimental Biology and Medicine 177:495–498
    [Google Scholar]
  12. LECATSAS G., TAYLOR M. B., LYONS S. F., SCHOUB B. D. 1986; Pleomorphism in HTLV-III, the AIDS virus. South African Medical Journal 69:793–794
    [Google Scholar]
  13. MACNAUGHTON M. R., DAVIES H. A. 1987 Corona viridae. Animal Virus Structure173–183 Edited by Nermut M., Steven A. Amsterdam: Elsevier;
    [Google Scholar]
  14. MUNN R. J., MARX P. A., YAMAMOTO J. K., GARDNER M. B. 1985; Ultrastructural comparison of the retroviruses associated with human and simian immunodeficiency syndromes. Laboratory Investigation 53:194–199
    [Google Scholar]
  15. PALMER E., SPORBORG C, HARRISON A., MARTIN M. L., FEORINO P. 1985; Morphology and immunoelectron microscopy of AIDS viruses. Archives of Virology 85:189–196
    [Google Scholar]
  16. POPOVIC M., READ-CANNOLE E., GALLO R. C. 1984a; T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III. Lancet ii:1472–1473
    [Google Scholar]
  17. POPOVIC M., SARNGADHARAN M. G., READ E., GALLO R. C. 1984b; Detection, isolation and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224:497–500
    [Google Scholar]
  18. ROSTGAARD I., TRANUM-JENSEN J. 1980; A procedure for minimizing cellular shrinkage in electron microscope preparation: a quantitative study on frog gall bladder. Journal of Microscopy 119:213–232
    [Google Scholar]
  19. SALAHUDDIN S. Z., MARKHAM P. D., WONG-STAAL F., FRANCHINI G., KALYANARAMAN V. S., GALLO R. C. 1983; Restricted expression of human T-cell leukemia-lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes. Virology 129:51–64
    [Google Scholar]
  20. SARKAR N. H., MOORE D. H. 1986; The internal structure of a mouse mammary tumor virus as revealed after Tween-ether treatment. Journal of Microscopy 7:539–548
    [Google Scholar]
  21. SCHNEIDER G. B. 1976; The effects of preparative procedures for scanning electron microscopy on the size of isolated lymphocytes (1). American Journal of Anatomy 146:93–100
    [Google Scholar]
  22. SCHNEIDER J., KAADEN O., COPELAND T. D., OROSZLAN S., HUNSMANN G. 1986; Shedding and interspecies type sero-reactivity of the envelope glycopolypeptide gpl20 of the human immunodeficiency virus. Journal of General Virology 67:2533–2538
    [Google Scholar]
  23. SCHNEIDER U., SCHWENK H. V., BORNKAMM G. 1977; Characterization of EBV-genome negative ‘Null’ and T’ cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. International Journal of Cancer 19:621–626
    [Google Scholar]
  24. SCHUPBACH I., POPOVIC M., GILDEN R. V., GONDA M. A., SARNGADHARAN M. G., GALLO R. C. 1984; Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS. Science 224:503–505
    [Google Scholar]
  25. STANBRIDGE E. J., WEISS R. L. 1978; Mycoplasma capping on lymphocytes. Nature, London 276:583–587
    [Google Scholar]
  26. STANNARD L. M., VAN DER RIET F. DE ST. J., MOODIE J. W. 1987; The morphology of human immunodeficiency virus particles by negative staining electron microscopy. Journal of General Virology 68:919–923
    [Google Scholar]
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