1887

Abstract

Summary

Eight measles virus strains including four subacute sclerosing panencephalitis (SSPE) isolates were compared on the basis of their growth characteristics in human peripheral blood mononuclear cells and effect on mitogen-stimulated lymphocyte proliferation. The Edmonston strain virus and some other strains of measles virus replicated in phytohaemagglutinin (PHA)-stimulated lymphocytes and released high titres of infectious virus into the culture medium. Inhibition of DNA synthesis was relatively low at the beginning of lymphocyte proliferation and could be detected only at high multiplicity of infection. Three to 4 days after initiation of proliferation a strong inhibition was seen also in cultures infected with low doses of virus, and was apparently related to the production of high titres of new infectious virus. Some virus strains originally isolated from SSPE patients produced only a small amount of infectious virus in lymphocytes at 37 °C but had a very strong inhibitory effect on lymphocyte proliferation leading to early cell death. The inhibitory effect was found over a wide range of virus concentrations, but was strictly dose-dependent and no increase in inhibition with low multiplicities of infection could be seen with longer culture times. The amount of interferon induced by different strains varied from 400 to 6400 international units/ml but the amount of interferon produced in the cultures did not correlate with the inhibitory effect on proliferation or with the amount of released new infectious virus. The Hallé measles strain, originally isolated from an SSPE patient, differed most from the Edmonston strain in its characteristics, and was studied in greater detail. Although only a small amount of infectious virus was produced, the Hallé strain had a very strong inhibiting effect on the proliferation of PHA-stimulated lymphocytes, and extensive syncytium formation was seen leading to cell death within 3 days.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-1-247
1988-01-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/1/JV0690010247.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-1-247&mimeType=html&fmt=ahah

References

  1. BASLE M. F., RUSSELL W. C, GOSWAMI K. K. A., REBEL A., GIRAUDON P., WILD F., FILMON R. 1985; Paramyxovirus antigens in osteoclasts from Paget’s bone tissue detected by monoclonal antibodies. Journal of General Virology 66:2103–2110
    [Google Scholar]
  2. BASLE M. F., FOURNIER J. G., ROZENBLATT S., REBEL A., BOUTEILLE M. 1986; Measles virus RNA detected in Paget’s disease bone tissue by in situ hybridization. Journal of General Virology 67:907–913
    [Google Scholar]
  3. CARRIGAN D. 1985; Round cell variant of measles virus: spontaneous conversion from productive to cell-associated state of infection. Virology 144:337–350
    [Google Scholar]
  4. FINKEL A., DENT P. B. 1973; Abnormalities in lymphocyte proliferation in classical and atypical measles infection. Cellular Immunology 6:41–48
    [Google Scholar]
  5. FOURNIER J-G., TARDIEU M., LEBON P., ROBAIN O., PONSOT G., ROZENBLATT S., BOUTEILLE M. 1985; Detection of measles virus RNA in lymphocytes from peripheral-blood and brain perivascular infiltrates of patients with subacute sclerosing panencephalitis. New England Journal of Medicine 313:910–915
    [Google Scholar]
  6. GRESSER I., BANDU M. T., BROUTY-BOYE D., TOVEY M. 1974; Pronounced antiviral activity of human interferon on bovine and porcine cells. Nature, London 251:543–547
    [Google Scholar]
  7. GRIFFITH J. F. 1985; Subacute sclerosing panencephalitis and lymphocytes. New England Journal of Medicine 313:952–954
    [Google Scholar]
  8. HOLLAND J. J., SPINDLER K. R., HORODYSKI F. M., GRABAU E. A., NICHOL S. T., VANDEPOL S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  9. HUGHES P. J., EVANS D. M. A., MINOR P. D., SCHILD G. C, ALMOND J. W., STANWAY G. 1986; The nucleotide sequence of a type 3 poliovirus isolated during a recent outbreak of poliomyelitis in Finland. Journal of General Virology 67:2093–2102
    [Google Scholar]
  10. LEBON P., PONSOT G., ALCARDI J., GOUTIERES F., ARTHUIS M. 1979; Early intrathecal synthesis of interferon in herpes encephalitis. Biomedicine 31:267–271
    [Google Scholar]
  11. LUCAS C. J., GALAMA J., UBELS-POSTMA J. 1977; Measles virus-induced suppression of lymphocyte reactivity in vitro. Cellular Immunology 32:70–85
    [Google Scholar]
  12. MARUSYK R. G., TYRRELL D. L. J. 1984 Measles: the virus, the disease and vaccine problems. Control of Viral Diseases67–78 Edited by Kurstak E., Marusyk R. G. New York: Marcel Dekker;
    [Google Scholar]
  13. SHESHBERADARAN H., NORRBY E. 1986; Characterization of epitopes on the measles virus hemagglutinin. Virology 152:58–65
    [Google Scholar]
  14. SULLIVAN J. L., BARRY D. W., ALBRECHT P., LUCAS S. J. 1975a; Measles infection of human mononuclear cells. I. Acute infection of peripheral blood lymphocytes and monocytes. Journal of Experimental Medicine 142:773–784
    [Google Scholar]
  15. SULLIVAN J. L., BARRY D. W., ALBRECHT P., LUCAS S. J. 1975b; Inhibition of lymphocyte stimulation by measles virus. Journal of Immunology 114:1458–1461
    [Google Scholar]
  16. TER MEULEN V., STEPHENSON J. R. 1983 The possible role of viral infections in multiple sclerosis and other related demyelinating diseases. Multiple Sclerosis241–274 Edited by Hallpike J. F., Adams C. W., Tourtellotte W. W. Baltimore: Williams & Wilkins;
    [Google Scholar]
  17. TSANG L. W-L., CHANG N., MARUSYK R. G. 1981; An interference phenomenon associated with a measles virus SSPE isolate (Hallé). Journal of General Virology 56:195–198
    [Google Scholar]
  18. VOLCKAERT-VERVLIET G., HEREMANS H., DE LEY M., BILLIAU A. 1978; Interferon induction and action in human lymphoblastoid cells infected with measles virus. Journal of General Virology 41:459–466
    [Google Scholar]
  19. VON PIRQUET C P. 1908; Das Verhalten der kutanen Tuberculinreaktion während der Masern. Deutsche medizinische Wochenschrift 34:1297–1300
    [Google Scholar]
  20. WECHSLER S. L., MEISSNER H. C. 1982 Measles and SSPE viruses: similarities and differences. Progress in Medical Virology 2865–95 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  21. ZEMAN W. 1978 In Handbook of Clinical Neurology 34343–368 Edited by Vinken P. J., Bruyn W. Amsterdam: North-Holland;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-1-247
Loading
/content/journal/jgv/10.1099/0022-1317-69-1-247
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error