Nucleotide Sequence of the Capsid Protein Gene and 3′ Non-coding Region of Papaya Mosaic Virus RNA Free

Abstract

Summary

The nucleotide sequences of cDNA clones corresponding to the 3′ OH end of papaya mosaic virus RNA have been determined. The 3′-terminal sequence obtained was 900 nucleotides in length, excluding the poly(A) tail, and contained an open reading frame capable of giving rise to a protein of 214 amino acid residues with an of 22930. This protein was identified as the viral capsid protein. The 3′ non-coding region of PMV genome RNA was about 121 nucleotides long [excluding the poly(A) tail] and homologous to the complementary sequence of the non-coding region at the 5′ end of PMV RNA. A long open reading frame was also found in the predicted 5′ end region of the negative strand.

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1988-01-01
2024-03-29
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References

  1. ABOUHAIDAR M. G. 1983; The structure of the 5′ and 3′ ends of clover yellow mosaic virus RNA. Canadian Journal of Microbiology 29:151–156
    [Google Scholar]
  2. ABOUHAIDAR M. G., BANCROFT J. B. 1978; The initiation of papaya mosaic virus assembly. Virology 90:54–59
    [Google Scholar]
  3. ABOUHAIDAR M. G., ERICKSON I. W. 1985 Structure and in vitro assembly of papaya mosaic virus. Molecular Plant Virology 185–121 Edited by Davies J. W. Boca Raton: CRC Press;
    [Google Scholar]
  4. AHLQUIST P., DASGUPTA R., KAESBERG P. 1984; Nucleotide sequence of the brome mosaic virus genome and its implication for viral replication. Journal of Molecular Biology 172:369–383
    [Google Scholar]
  5. BENDENA W. G., ABOUHAIDAR M. G., MACKIE G. A. 1985; Synthesis in vitro of the coat protein of papaya mosaic virus. Virology 140:257–268
    [Google Scholar]
  6. BIRNBOIM H. C., DOLY J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  7. BLOOMER A. C, CHAMPNESS I. N., BRICOGNE G., STADEN R., KLUG A. 1978; Protein disk of tobacco mosaic virus at 2-8 A resolution showing the interactions within and between subunits. Nature, London 276:362–368
    [Google Scholar]
  8. BOUZOUBAA S., GUILLEY H., JONARD G., RICHARDS K., PUTZ C. 1985; Nucleotide sequence analysis of RNA-3 and RNA-4 of beet necrotic yellow vein virus, isolates F2 and G1. Journal of General Virology 66:1553–1564
    [Google Scholar]
  9. BUELL G. N., WICKENS M. P., PAYVAR F., SCHIMKE R. T. 1978; Synthesis of full-length cDNAs from four partially purified oviduct mRNAs. Journal of Biological Chemistry 253:2471–2482
    [Google Scholar]
  10. DAYHOFF M. O. editor 1976 Atlas of Protein Sequence and Structure 5 supplement 2 3–9 Silver Spring: National Biomedical Research Foundation;
    [Google Scholar]
  11. DOMIER L. L., FRANKLIN K. M., SHAHABUDDIN M., HELLMANN G. M., ÖVÉRMEYER J. H., HIREMATH S. T., SIAW M. F. E., LOMONOSSOFF G. P., SHAW J. G., RHOADS R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  12. ERICKSON J. W. 1978 The self-assembly of papaya mosaic virus Ph.D. thesis, University of Western Ontario
    [Google Scholar]
  13. ERICKSON J. W., ABOUHAIDAR M. G., BANCROFT J. B. 1978; The specificity of papaya mosaic virus assembly. Virology 90:60–66
    [Google Scholar]
  14. GOELET P., LOMONOSSOFF G. P., BUTLER P. J. G., AKAM M. E., GAIT M. J., KARN J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U.S.A 79:5818–5822
    [Google Scholar]
  15. GOODMAN H. M., MacDONALD R. J. 1979; Cloning of hormone genes from a mixture of cDNA molecules. Methods in Enzymology 68:75–90
    [Google Scholar]
  16. GUBLER U., HOFFMAN B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  17. LOK S., ABOUHAIDAR M. G. 1986; The nucleotide sequence of the 5′ end of papaya mosaic virus RNA: site of in vitro assembly initiation. Virology 153:289–296
    [Google Scholar]
  18. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. MESHI T., KIYAMA R., OHNO T., OKADA Y. 1983; Nucleotide sequence of the coat protein cistron and the 3′ noncoding region of the cucumber green mottle mosaic virus (watermelon strain) RNA. Virology 127:54–64
    [Google Scholar]
  20. MICHELSON A. M., ORKIN S. H. 1982; Characterization of the homopolymer tailing reaction catalyzed by terminal deoxynucleotidyl transferase. Journal of Biological Chemistry 257:14773–14782
    [Google Scholar]
  21. MOROZOV S. YU., GORBULEV V. G., NOVIKOV V. K., AGRANOVSKI A. A., KOZLOV YU. V., ATABEKOV J. G., BAYEV A. A. 1981; The primary structure of the 5′ and 3′ terminal regions of the genomic RNA of potato virus X. Proceedings of the Academy of Sciences, U.S.S.R 259:723–725
    [Google Scholar]
  22. MOROZOV S. YU., ZAKHARIEV V. M., CHERNOV B. K., PRASOLOV V. S., KOZLOV YU. V., ATABEKOV J. G., SKRYABIN K. G. 1983; Analysis of the primary structure and localization of the capsid protein gene in the RNA genome of potato virus X. Proceedings of the Academy of Sciences, U.S.S.R 271:211–215
    [Google Scholar]
  23. NEVINS J. R. 1983; The pathway of eucaryotic mRNA formation. Annual Review of Biochemistry 52:441–466
    [Google Scholar]
  24. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  25. SAWYER L., TOLLIN P., WILSON H. R. 1987; A comparison between the predicted secondary structures of potato virus X and papaya mosaic virus coat proteins. Journal of General Virology 68:1229–1232
    [Google Scholar]
  26. SHORT M. N., TURNER D. S., MARCH J. F., PAPPIN D. J. C, PARENTE A., DAVIES J. W. 1986; The primary structure of papaya mosaic virus coat protein. Virology 152:280–283
    [Google Scholar]
  27. SOUTHERN E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  28. TOLLIN P., BANCROFT J. B., RICHARDSON J. F., PAYNE N. C., BEVERIDGE T. J. 1979; Diffraction studies of papaya mosaic virus. Virology 98:108–115
    [Google Scholar]
  29. WILBUR W. J., LIPMAN D. J. 1983; Rapid similarity searches of nucleic acid and protein data banks. Proceedings of the National Academy of Sciences, U.S.A 80:726–730
    [Google Scholar]
  30. YANISCH-PERRON C, VIEIRA J., MESSING J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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