1887

Journal of General Virology

Volume 69, Issue 1

Isolation and Characterization of Thermosensitive Mutants from Kilham Rat Virus, a Rodent Parvovirus Free

Abstract

Summary

Thermosensitive mutants were isolated from nitrous acid-treated Kilham rat virus (KRV). At a restrictive temperature (39.5 °C), the mutants tested did not produce appreciable amounts of infectious particles, haemagglutinin or progeny single-stranded DNA. Virus antigen accumulation as detected by immunoperoxidase was reduced. Complementation tests revealed two distinct groups. The three members of complementation group 1 synthesized normal amounts of replicative forms but were restricted in single-stranded DNA production and capsid protein expression, exhibiting phenotypes compatible with cap mutations. The sole representative of group 2, KRV 6, failed to accumulate replicative forms, displaying a rep phenotype. These mutants provide new tools to test the role of viral products in the biology of autonomous parvoviruses.

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Keyword(s): Kilham rat virus , parvovirus and ts mutants
© Society for General Microbiology 1988
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1988-01-01
2021-10-26
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Isolation and Characterization of Thermosensitive Mutants from Kilham Rat Virus, a Rodent Parvovirus
J. Gen. Virol. 69, 163 (1988); https://doi.org/10.1099/0022-1317-69-1-163
/content/journal/jgv/10.1099/0022-1317-69-1-163
/content/journal/jgv/10.1099/0022-1317-69-1-163
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