1887

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Volume 69, Issue 1

Reassortant Formation and Selection Following Coinfection of Cultured Cells with Subgroup 2 Human Rotaviruses Free

Abstract

Summary

Reassortant formation following coinfection has been suggested as a mechanism of evolution of rotaviruses. This study was designed to examine the selection of reassortants following coinfection of cultured cells with pairs of subgroup 2 human rotaviruses. The three pairs studied (Wa × P, CJN × 31, 62 × 69) were chosen to maximize the number of RNA segments that could be electrophoretically distinguished. After coinfection and multiple passages, reproducible selection of reassortants was observed with each pair. Although more segments were selected from the virus of a pair that grew to higher titre, certain segments were selected independently of the relative growth properties or multiplicities of infection of the coinfecting viruses; selection of other segments was dependent on both. In determining the time and cause of selection it was found that no selection of genomic RNA segments was detectable prior to or during viral particle assembly in coinfected cells. However, selection was evident within the infectious progeny population after a single cycle of replication. Therefore, selection of specific reassortants following coinfection was apparently due to differences in the infectivities of progeny viruses and not in their assembly. This implies that these infectivities were a function of the parental origin of specific genomic segments.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): human , reassortment , rotavirus and selection
© Society for General Microbiology 1988
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1988-01-01
2024-04-16
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References

  1. BOHL E. H., THEIL K. W., SAIF L. J. 1984; Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. Journal of Clinical Microbiology 19:105–111
     [Google Scholar]
  2. BOTH G. W., SIEGMAN L. J., BELLAMY A. R., ATKINSON P. H. 1983; Coding assignment and nucleotide sequence of simian rotavirus SAH gene segment 10: location of glycoprotein sites suggests that the signal peptide is not cleaved. Journal of Virology 48:335–339
     [Google Scholar]
  3. DYALL-SMITH M. L., HOLMES I. H. 1981; Gene-coding assignments of rotavirus double-stranded RNA segments 10 and 11. Journal of Virology 38:1099–1103
     [Google Scholar]
  4. ERICSON B. L., GRAHAM D. Y., MASON B. B., HANSSEN H. H., ESTES M. K. 1983; Two types of glycoprotein precursors are produced by the simian rotaviruses SAIL. Virology 127:320–332
     [Google Scholar]
  5. ESTES M. K., GRAHAM D. Y., DIMITROV D. H. 1984; The molecular epidemiology of rotavirus gastroenteritis. Progress in Medical Virology 29:1–22
     [Google Scholar]
  6. FLORES J., MIDTHUN K., HOSHINO Y., GREEN K., GORZIGLIA M., KAPIKIAN A. Z., CHANOCK R. M. 1986; Conservation of the fourth gene among rotaviruses recovered from asymptomatic newborn infants and its possible role in attenuation. Journal of Virology 60:972–979
     [Google Scholar]
  7. GARBARG-CHENON A., BRICOUT F., NICOLAS J.-C. 1984; Study of genetic reassortment between two human rotaviruses. Virology 139:358–365
     [Google Scholar]
  8. GARBARG-CHENON A., BRICOUT F., NICOLAS J.-C. 1986; Serological characterization of human reassortant rotaviruses. Journal of Virology 59:510–513
     [Google Scholar]
  9. GOMBOLD J. L., RAMIG R. F. 1986; Analysis of reassortment of genome segments in mice mixedly infected with rotavirus SAH and RRV. Journal of Virology 57:110–116
     [Google Scholar]
  10. GORZIGLIA M., HOSHINO Y., BUCKLER-WHITE A., GLASS R., FLORES J., KAPIKIAN A. Z., CHANOCK R. M. 1986; Conservation of the amino acid sequence of VP3 and cleavage of the 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proceedings of the National Academy of Sciences, U.S.A 83:7039–7043
     [Google Scholar]
  11. GRAHAM A., KUDESIA G., ALLEN A. M., DESSELBERGER U. 1987; Reassortment of human rotavirus possessing genome rearrangements with bovine rotavirus: evidence for host cell selection. Journal of General Virology 68:115–122
     [Google Scholar]
  12. GREENBERG H. B., FLORES J., KALICA A. R., WYATT R. G., JONES R. 1983; Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. Journal of General Virology 64:313–320
     [Google Scholar]
  13. HOSHINO Y., SERENO M. M., MIDTHUN K., FLORES J., CHANOCK R. G., KAPIKIAN A. Z. 1987; Analysis by plaque reduction neutralization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. Journal of Clinical Microbiology 25:290–294
     [Google Scholar]
  14. KALICA A. R., GREENBERG H. B., WYATT R. G., FLORES J., SERENO M. M., KAPIKIAN A. Z., CHANOCK R. M. 1981; Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112:385–390
     [Google Scholar]
  15. LAEMMLI U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  16. LUBECK M. D., PALESE P., SCHULMAN J. L. 1979; Nonrandom association of parental genes in influenza A virus recombinants. Virology 95:269–274
     [Google Scholar]
  17. MIDTHUN K., VALDESUSO J., HOSHINO Y., FLORES J., KAPIKIAN A. Z., CHANOCK R. M. 1987; Analysis by RNA-RNA hybridization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. Journal of Clinical Microbiology 25:295–300
     [Google Scholar]
  18. NICOLAS J. C, POTHIER P., COHEN J., LOURENCO M. H., THOMPSON R., GUIMBAUD P., CHENON A., DAUVERGNE M., BRICOUT F. 1984; Survey of human rotavirus propagation as studied by electrophoresis of genome RNA. Journal of Infectious Diseases 149:688–693
     [Google Scholar]
  19. OFFIT P. A., BLAVAT G., GREENBERG H. B., CLARK H. F. 1986; Molecular basis of rotavirus virulence: role of gene segment 4. Journal of Virology 57:46–49
     [Google Scholar]
  20. OKADA Y., RICHARDSON M. A., IKEGAMI N., NOMOTO A., FURUICHI Y. 1984; Nucleotide sequence of human rotavirus genome segment 10, an RNA encoding a glycosylated virus protein. Journal of Virology 51:856–859
     [Google Scholar]
  21. RODRIGUEZ W. J., KIM H. W., BRANDT C D., GARDNER M. K., PARROTT R. H. 1983; Use of electrophoresis of RNA from human rotavirus to establish the identity of strains involved in outbreaks in a tertiary care nursery. Journal of Infectious Diseases 148:34–40
     [Google Scholar]
  22. SPENCER E. G., AVENDANO L. F., GARCIA B. I. 1983; Analysis of human rotavirus mixed electropherotypes. Infection and Immunity 39:569–574
     [Google Scholar]
  23. URASAWA S., URASAWA T., TANIGUCHI K. 1986; Genetic reassortment between two human rotaviruses having different serotype and subgroup specificities. Journal of General Virology 67:1551–1559
     [Google Scholar]
  24. WARD R. L., KNOWLTON D. R., PIERCE M. J. 1984; Efficiency of human rotavirus propagation in cell culture. Journal of Clinical Microbiology 19:748–753
     [Google Scholar]
  25. WARD R. L., BERNSTEIN D. I., YOUNG E. C, SHERWOOD J. R., KNOWLTON D. R., SCHIFF G. M. 1986; Human rotavirus studies in volunteers: determination of infectious doses and serological response to infection. Journal of Infectious Diseases 154:871–880
     [Google Scholar]
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Reassortant Formation and Selection Following Coinfection of Cultured Cells with Subgroup 2 Human Rotaviruses
J. Gen. Virol. 69, 149 (1988); https://doi.org/10.1099/0022-1317-69-1-149
/content/journal/jgv/10.1099/0022-1317-69-1-149
/content/journal/jgv/10.1099/0022-1317-69-1-149
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