1887

Abstract

Summary

Viruses which belong to the epizootic haemorrhagic disease (EHD), bluetongue and Eubenangee serogroups of orbiviruses exhibit low level cross-reactions in some serological tests. Although Pata virus cross-reacts at low levels with members of the EHD and bluetongue serogroups, it was assigned originally to the Eubenangee serogroup. RNA-RNA blot hybridization data, however, suggest that Pata virus is not a member of the Eubenangee serogroup. In this study, the genetic relatedness of the EHD serogroup viruses, bluetongue virus type 10 (BTV-10) and Pata virus was assessed by RNA-RNA blot hybridization and by gene reassortment experiments . The five members of the EHD serogroup examined were highly related by reciprocal RNA-RNA blot hybridization. Genes 1, 3, 4 and 6 to 9 were highly conserved with three unique types of gene 2, four variant types of gene 5 and two variant types of gene 10. Geographical boundaries could not be correlated with sequence relatedness because viruses isolated from the same locality were distant relatives when compared with another virus isolated on a different continent. The significance of the unique and variant genes is discussed. The EHD isolates, BTV-10 and Pata virus exhibited distinct profiles on agarose and polyacrylamide gel electrophoresis, and they are related distantly as shown by weak hybridization signals in blot hybridization. Gene 2 was a unique gene among the EHD isolates, BTV-10 and Pata virus. One BTV-10 gene hybridized more strongly to gene 9 of the EHD viruses than the other BTV-10 genes, and its role in encoding the cross-reactive antigen is discussed. Intra-serogroup gene reassortment was demonstrated in the six crosses among EHD serogroup members. In contrast, gene reassortment was not observed in inter-serogroup crosses between EHD 1 and BTV-10, BTV-10 and Pata virus, and between EHD 1 and Pata virus. Correlation of blot hybridization and gene reassortment indicated that viruses must share high sequence conservation in the majority of their genes before genetic interaction is likely. The usefulness of blot hybridization as an indicator of the likelihood of gene reassortment is discussed. These hybridization and gene reassortment data indicated that Pata virus is not a member of the bluetongue or EHD serogroups and that it should be assigned to the ungrouped set of orbiviruses. The hybridization and gene reassortment data suggest that members of the EHD serogroup, BTV-10 and Pata virus represent three distinct gene pools, and the role of reassortment in the generation of genetic diversity is discussed. The minor antigens shared among these gene pools probably reflect their common ancestry.

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1988-01-01
2024-03-28
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References

  1. BODKIN D. K., KNUDSON D. L. 1985a; Assessment of sequence relatedness of dsRNA genes by RNA-RNA blot hybridization. Journal of Virological Methods 10:45–52
    [Google Scholar]
  2. BODKIN D. K., KNUDSON D. L. 1985b; Sequence relatedness of Palyam virus genes to cognates of the Palyam serogroup viruses by RNA-RNA blot hybridization. Virology 143:55–62
    [Google Scholar]
  3. BODKIN D. K., KNUDSON D. L. 1986; Genetic relatedness of Palyam serogroup viruses by RNA-RNA blot hybridization. Journal of General Virology 67:683–691
    [Google Scholar]
  4. BODKIN D. K., KNUDSON D. L. 1987; Genetic relatedness of Colorado tick fever virus isolates by RNA-RNA blot hybridization. Journal of General Virology 68:1199–1204
    [Google Scholar]
  5. BORDEN E. C, SHOPE R. E., MURPHY F. A. 1971; Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus - a new taxonomic group. Physicochemical and serological studies. Journal of General Virology 13:261–271
    [Google Scholar]
  6. BUCKLEY S. M. 1974; Cross plaque neutralization tests with cloned Crimean hemorrhagic fever-Congo (CHF-C) and Hazara viruses (38154). Proceedings of the Society for Experimental Biology and Medicine 146:594–600
    [Google Scholar]
  7. CAMPBELL C. H., ST. GEORGE T. D. 1986; A preliminary report of a comparison of epizootic haemorrhagic disease viruses from Australia with others from North America, Japan and Nigeria. Australian Veterinary Journal 63:233
    [Google Scholar]
  8. CAMPBELL C. H., BARBER T. L., JOCHIM M. M. 1978; Antigenic relationship of Ibaraki, bluetongue, and epizootic hemorrhagic disease viruses. Veterinary Microbiology 3:15–22
    [Google Scholar]
  9. DELLA-PORTA A. J. 1985 Classification of orbiviruses: a need for supergroups or genera. Progess in Clinical and Biological Research vol. 178: Bluetongue and Related Orbiviruses267–274 Edited by Barber T. L., Jochim M. M. New York: Alan R. Liss;
    [Google Scholar]
  10. DONIS-KELLER H. 1979; Site specific enzymatic cleavage of RNA. Nucleic Acids Research 7:179–192
    [Google Scholar]
  11. ERASMUS B. J. 1985 The history of bluetongue. Progress in Clinical and Biological Research, vol. 178: Bluetongue and Related Orbiviruses7–12 Edited by Barber T. L., Jochim M. M. New York: Alan R. Liss;
    [Google Scholar]
  12. FOSTER N. M., METCALF H. E., BARBER T. L., JONES R. H., LUEDKE A. J. 1980; Bluetongue and epizootic hemorrhagic disease virus isolations from vertebrate and invertebrate hosts at a common geographic site. Journal of the American Veterinary Medical Association 176:126–129
    [Google Scholar]
  13. GAILLARD R. K. JR, JOKLIK W. K. 1982; Quantitation of the relatedness of reovirus serotypes 1, 2, and 3 at the gene level. Virology 123:152–164
    [Google Scholar]
  14. GONZALEZ H. A., KNUDSON D. L. 1987; Genetic relatedness of Corriparta serogroup viruses. Journal of General Virology 68:661–672
    [Google Scholar]
  15. GONZALEZ H. A., KNUDSON D. L. 1988; Intra- and inter-serogroup genetic relatedness of orbiviruses. I. Blot hybridization of viruses of Australian serogroups. Journal of General Virology 69:125–134
    [Google Scholar]
  16. GORMAN B. M. 1979; Variation in orbiviruses. Journal of General Virology 44:1–15
    [Google Scholar]
  17. GORMAN B. M., TAYLOR J. 1978; The RNA genome of Tilligerry virus. Australian Journal of Experimental Biology and Medical Science 56:369–371
    [Google Scholar]
  18. GORMAN B. M., TAYLOR J., WALKER P. J., YOUNG P. R. 1978; The isolation of recombinants between related orbiviruses. Journal of General Virology 41:333–342
    [Google Scholar]
  19. GORMAN B. M., TAYLOR J., WALKER P. J. 1983 Orbiviruses. Reoviridae287–357 Edited by Joklik W. K. New York: Plenum Press;
    [Google Scholar]
  20. HUISMANS H., ERASMUS B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
    [Google Scholar]
  21. HUISMANS H., BREMER C. W., BARBER T. L. 1979; The nucleic acid and proteins of epizootic hemorrhagic disease virus. Onderstepoort Journal of Veterinary Research 46:95–104
    [Google Scholar]
  22. KAHLON J., SUGIYAMA K., ROY P. 1983; Molecular basis of bluetongue virus neutralization. Journal of Virology 48:627–632
    [Google Scholar]
  23. KNUDSON D. L. 1981; Genome of Colorado tick fever. Virology 112:361–364
    [Google Scholar]
  24. KNUDSON D. L., SHOPE R. E. 1985 Overview of the orbiviruses. Progress in Clinical and Biological Research, vol. 178: Bluetongue and Related Orbiviruses255–266 Edited by Barber T. L., Jochim M. M. New York: Alan R. Liss;
    [Google Scholar]
  25. KNUDSON D. L., BUTTERFIELD W. K., SHOPE R. E., WALTON T. E., CAMPBELL C. H. 1982; Electrophoretic comparison of the genomes of North American viruses, one Australian Bluetongue, and three other related orbiviruses. Veterinary Microbiology 7:285–293
    [Google Scholar]
  26. KOWALIK T. K., LI J. K. 1987; The genetic relatedness of United States prototype bluetongue viruses by RNA-RNA hybridization. Virology 158:276–284
    [Google Scholar]
  27. LEE V. H., CAUSEY O. R., MOORE D. L. 1974; Bluetongue and related viruses in Ibadan, Nigeria: isolation and preliminary identification of viruses. American Journal of Veterinary Research 35:1105–1108
    [Google Scholar]
  28. MOORE D. L. 1974; Bluetongue and related viruses in Ibadan, Nigeria: serologic comparisons of bluetongue, epizootic hemorrhagic disease of deer and Abadina (Palyam) viral isolates. American Journal of Veterinary Research 35:1109–1113
    [Google Scholar]
  29. MOORE D. L., LEE V. H. 1972; Antigenic relationship between the virus of epizootic haemorrhagic disease of deer and bluetongue virus. Archiv für die gesamte Virusforschung 37:282–284
    [Google Scholar]
  30. RAMIG R. F., CROSS R. K., FIELDS B. N. 1977; Genome RNAs and polypeptides of reovirus serotypes 1,2, and 3. Journal of Virology 22:726–733
    [Google Scholar]
  31. SHOPE R. E., MACNAMARA L. G., MANGOLD R. 1955; Deer mortality-epizootic hemorrhagic disease of deer. New Jersey Outdoors 6:17–20
    [Google Scholar]
  32. SHOPE R. E., MACNAMARA L. G., MANGOLD R. 1960; A virus-induced epizootic hemorrhagic disease of the Virginia white-tailed deer (Odocoileus virginianus). Journal of Experimental Medicine 111:155–170
    [Google Scholar]
  33. STOKER M., MACPHERSON I. 1961; Studies on transformation of hamster cells by polyoma virus in vitro. Virology 14:359–370
    [Google Scholar]
  34. TESH R. B., PELEG J., SAMINA I., MARGALIT J., BODKIN D. K., SHOPE R. E., KNUDSON D. 1986; Biological and antigenic characterization of Netivot virus, an unusual new Orbivirus recovered from mosquitoes in Israel. American Journal of Tropical Medicine and Hygiene 35:418–428
    [Google Scholar]
  35. TRAVASSOS DA, ROSA A. P., TESH R. B., PINHEIRO F. P., TRAVASSOS DA ROSA J. F., PERALTA P. H., KNUDSON D. L. 1984; Characterization of the Changuinola serogroup viruses (Reoviridae: Orbivirus). Intervirology 21:38–49
    [Google Scholar]
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