1887

Abstract

Summary

A simple and accurate immunocytochemical focus assay is described, whereby both transforming and non-transforming avian retroviruses can be enumerated. After virus infection of chick embryo fibroblasts, an agar overlay is applied; foci of infected cells (expression foci) are detected immunocytochemically after 5 to 7 days. The primary antibodies are monoclonal sera directed against either viral p 19 or pp60. Detection of expression foci after transfection of cells with cloned viral DNA is also demonstrated.

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/content/journal/jgv/10.1099/0022-1317-68-9-2481
1987-09-01
2022-01-25
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