1887

Abstract

Summary

Sera were produced by injection of a particular rat cell line (HLBRK C1), produced by transformation of baby rat kidney cells with adenovirus 12 early region 1 DNA, into the syngeneic host. Antibodies to a protein of 15200 as determined by SDS-PAGE, were detected in the sera from two rats bearing tumours. The 15K protein was identified as the viral structural component polypeptide IX by a number of criteria: (i) the protein was only observed in adenovirus 12-infected cells, (ii) it was produced at intermediate and late times after infection, (iii) it was immunoprecipitated from purified adenovirus 12 particles and (iv) it was immunoprecipitated from a purified protein preparation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-9-2471
1987-09-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/9/JV0680092471.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-9-2471&mimeType=html&fmt=ahah

References

  1. Anderson C. W., Schmitt R. C., Smart J. E., Lewis J. B. 1984; Early region lb of adenovirus 2 encodes two coterminal proteins of 495 and 155 amino acid residues. Journal of Virology 50:387–396
    [Google Scholar]
  2. Berk A. J., Sharp P. A. 1978; Structure of the adenovirus 2 early mRNAs. Cell 14:695–711
    [Google Scholar]
  3. Bernards R., Vandereb A. J. 1984; Adenovirus transformation and oncogenicity. Biochimica et biophysica acta 783:187–204
    [Google Scholar]
  4. Bos J. L., Polder L. J., Bernards R., Schrier P., Van Den Elsen P. J., Van Der Eb A. J., Van Ormondt H. 1981; The 2-2 Kb Elb mRNA of human Ad 12 and Ad 5 codes for two tumor antigens starting at different AUG triplets. Cell 27:121–131
    [Google Scholar]
  5. Branton P. E., Bavley S. T., Graham F. L. 1985; Transformation by human adenoviruses. Biochimica et biophysica acta 780:67–94
    [Google Scholar]
  6. Colby W. W., Shenk T. 1981; Adenovirus type 5 virions can be assembled in vivo in the absence of detectable polypeptide IX. Journal of Virology 39:977–980
    [Google Scholar]
  7. Everitt E., Philipson L. 1974; Structural proteins of Adenoviruses. IX. Purification of three low molecular weight virion proteins of Adenovirus type 2 and their synthesis during productive infection. Virology 62:253–269
    [Google Scholar]
  8. Gallimore P. H., Byrd P. I., Grand R. J. A. 1985a; Adenovirus genes involved in transformation. What determines the oncogenic phenotype? In Viruses and Cancer pp 125–172 Rigby P. W. J., Wilkie N. M. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  9. Gallimore P. H., Byrd P. J., Whittaker J. L., Grand R. J. A. 1985b; The properties of rat cells transformed by DNA plasmids containing adenovirus type 12 DNA or specific fragments of the El region: a comparison of transforming frequencies. Cancer Research 45:2670–2680
    [Google Scholar]
  10. Gallimore P. H., Williams I., Breiding D., Grand R. J. A., Rowe M., Byrd P. 1986; Studies on adenovirus type-12 El region: gene expression, transformation of human and rodent cells, and malignancy. Cancer Cells 4:339–348
    [Google Scholar]
  11. Grand R. J. A. 1987; The structure and functions of Adenovirus 12 early region 1 proteins. Biochemical Journal 241:25–38
    [Google Scholar]
  12. Grand R. J. A., Gallimore P. H. 1984; Adenovirus type 12 early region 1 proteins: a study of their subcellular localization and protein-protein interactions. Journal of General Virology 65:2149–2166
    [Google Scholar]
  13. Grand R. J. A., Gallimore P. H. 1986; Modulation of the level of expression of cellular genes in adenovirus 12 infected and transformed human cells. EMBO Journal 5:1253–1260
    [Google Scholar]
  14. Jochemsen H., Daniels G. S. G., Hertoghs J. J. L., Schrier P. I., Van Den Elsen P. J., Van Der Eb A. J. 1982; Identification of adenovirus type 12 gene products involved in transformation and oncogenesis. Virology 122:15–28
    [Google Scholar]
  15. Katze M. G., Persson H., Philipson L. 1982; A novel mRNA and a low molecular weight polypeptide encoded in the transforming region of adenovirus DNA. EMBO Journal 1:783–790
    [Google Scholar]
  16. Lewis J. B., Mathews M. B. 1981; Viral messenger RNAs in six lines of adenovirus-transformed cells. Virology 115:345–360
    [Google Scholar]
  17. Mak S., Mak I. 1986; Oncogenic transformation by human adenoviruses and their DNA. In Adenovirus DNA pp 367–408 Doerfler W. Edited by Boston: Martinus Nijhoff;
    [Google Scholar]
  18. Paraskeva C., Brown K. W., Gallimore P. H. 1982; Adenovirus cell interactions early after infection : in vitrocharacteristics and tumourigenicity of adenovirus type 2-transformed rat liver epithelial cells. Journal of General Virology 58:73–81
    [Google Scholar]
  19. Persson H., Pettersson U., Mathews M. B. 1978; Synthesis of a structural adenovirus polypeptide in the absence of viral DNA replication. Virology 90:67–79
    [Google Scholar]
  20. Shiroki K., Shimojo H., Sawada Y., Uemizu Y., Fujinaga K. 1979; Incomplete transformation of rat cells by a small fragment of adenovirus 12 DNA. Virology 95:127–136
    [Google Scholar]
  21. Virtanen A., Pettersson U. 1985; Organization of early region IB of human adenovirus type 2 : identification of four differentially spliced mRNAs. Journal of Virology 54:383–391
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-9-2471
Loading
/content/journal/jgv/10.1099/0022-1317-68-9-2471
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error