1887

Abstract

Summary

We have tested a number of herpes simplex virus type 1 (HSV-1) populations for their ability to enter and express virus polypeptides in non-permissive rat XC cells. The viruses tested included 40 intratypic F(MP) recombinants and different batches of virus, belonging to the same strains, that had been produced in two different permissive systems (HEp-2 or Vero cells). Our results indicated that the ability to infect XC cells was determined in part by genetic elements of the virus genome and in part by phenotypic characteristics conferred by the permissive cell that had produced the virus: a virus strain like HSV-1 MP which, when produced in HEp-2 cells, was able to infect XC cells, lost this ability when produced in Vero cells. Working only with viruses produced in HEp-2 cells we showed that the ability to enter XC cells could be transferred from the MP strain to the F strain (which does not normally infect XC cells efficiently) by transfer of the cloned HI B (map units 0·745 to 0·81) or HI L (map units 0·706 to 0·745) fragments isolated from MP DNA. The implicated locus or loci seemed to segregate, however, from loci controlling gC synthesis and cell fusion, which have been described as mapping in the same region.

Keyword(s): HSV-1 , penetration and XC cells
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-9-2455
1987-09-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/9/JV0680092455.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-9-2455&mimeType=html&fmt=ahah

References

  1. Becker Y., Shlomai J., Asher Y., Weinberg E., Cohen Y., Olshevsky U., Kotler M. 1974; Interaction of herpes simplex type 1 with Rous sarcoma virus transformed rat cells (XC and R(B77) cell lines). Intervirology 4:325–332
    [Google Scholar]
  2. Bond V. C., Person S. 1984; Fine structure physical map locations of alterations that affect cell fusion in herpes simplex virus type 1. Virology 132:368–376
    [Google Scholar]
  3. Buckmaster E. A., Gompels U., Minson A. 1984; Characterisation and physical mapping of an HSV-1 glycoprotein of approximately 115 × 103 molecular weight. Virology 139:408–413
    [Google Scholar]
  4. Debroy C., Pederson N., Person S. 1985; Nucleotide sequence of a herpes simplex virus type-1 gene that causes cell fusion. Virology 145:36–48
    [Google Scholar]
  5. Deluca N., Bzik D., Bond V. C., Person S., Snipes W. 1982; Nucleotide sequences of herpes simplex virus type 1 affecting virus entry, cell fusion and production of glycoprotein gB. Virology 122:411–423
    [Google Scholar]
  6. Epstein A. L., Jacquemont B. 1983; Virus polypeptide synthesis induced by herpes simplex virus in nonpermissive rat XC cells. Journal of General Virology 64:1499–1508
    [Google Scholar]
  7. Epstein A., Jacquemont B., Machuca I. 1983; Differences in penetration into non-permissive XC cells between various strains of herpes simplex virus type 1. Annales de Virologie 134:439–453
    [Google Scholar]
  8. Epstein A., Jacquemont B., Machuca I. 1984; Infection of a restrictive cell line (XC cells) by intratypic recombinants of HSV-1: relationship between penetration of the virus and relative amounts of glycoprotein C. Virology 132:315–324
    [Google Scholar]
  9. Epstein A. L., Jacquemont B., Patet J. 1985; Susceptibility to herpes simplex virus type 1 infection of nonpermissive rat XC(HPRT-) × permissive mouse L(TK-) hybrid cells. Journal of General Virology 66:1805–1809
    [Google Scholar]
  10. Machuca I., Jacquemont B., Epstein A. 1986; Multiple adjacent or overlapping loci affecting the level of gC and cell fusion mapped by intratypic recombinants of HSV-1. Virology 150:117–125
    [Google Scholar]
  11. Noble A. G., Lee G.T-Y., Sprague R., Parish M. L., Spear P. G. 1983; Anti-gD monoclonal antibodies inhibit cell fusion induced by herpes simplex virus type 1. Virology 129:218–224
    [Google Scholar]
  12. Pereira L., Dondero D., Norrild B., Roizman B. 1981; Differential immunologic reactivity and processing of glycoproteins gA and gB of herpes simplex virus types 1 and 2 made in Vero and HEp-2 cells. Proceedings of the National Academy of Sciences U.S.A.: 785202–5206
    [Google Scholar]
  13. Pereira L., Dondero D., Roizman B. 1982; Herpes simplex virus glycoprotein gA/B: evidence that the infected Vero cell products comap and arise by proteolysis. Journal of Virology 44:88–97
    [Google Scholar]
  14. Ruyechan W. T., Morse L. S., Knipe D. M., Roizman B. 1979; Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and the genetic loci specifying the social behavior of infected cells. Journal of Virology 29:677–697
    [Google Scholar]
  15. Sarmiento M., Haffey M., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7(B2) in virion infectivity. Journal of Virology 29:1149–1158
    [Google Scholar]
  16. Zezulak K. M., Spear P. G. 1984; Limited proteolysis of herpes simplex virus glycoproteins that occurs during their extraction from Vero cells. Journal of Virology 50:258–268
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-9-2455
Loading
/content/journal/jgv/10.1099/0022-1317-68-9-2455
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error