1887

Abstract

Summary

Bacteriophage X1743D a high temperature-resistant mutant of X1743, was 10 times more stable than 0 X1743 as judged by its survival ratio after heat treatment at 54 °C for 120 min. Complementation tests showed an involvement of gene G. Sequence analysis of this gene revealed three mutation sites, one transition and two insertions. The first was a silent mutation and the others brought about a change in one amino acid and an addition of another, respectively, in the gene G protein. These changes in amino acid sequence resulted in a change in the secondary structure of the protein. A β-turn region in part of the gene G protein of X1743 was changed to an α-helix in X1743D These results indicate that the temperature resistance of X1743D may be caused by elevated hydrophobicity in the mutated region or by strong interaction between the mutated gene G protein and other capsid proteins.

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/content/journal/jgv/10.1099/0022-1317-68-9-2443
1987-09-01
2022-11-28
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