Induction of the major stress response in chick embryo fibroblasts, which follows infection at 38·5 °C with the herpes simplex virus mutant isK, was investigated. Synthesis of cellular stress proteins occurred only when the mutant form of an immediate early polypeptide, Vmwl75, was overproduced. Infection with mutant inl411, which has an amber (TAG) termination signal inserted between codons 83 and 84 of the gene encoding Vmwl75 and therefore specifies a truncated portion of the polypeptide, failed to stimulate stress protein synthesis. The results suggested that the presence of abnormal forms of Vmwl75 at high concentrations was the signal for induction of the stress response in tsK-infected cells.
DavidsonI., StowN. D.1985; Expression of an immediate early polypeptide and activation of a viral origin of DNA replication in cells containing a fragment of herpes simplex virus DNA. Virology 141:77–88
DelucaN. A., McCarthyA. M., SchafferP. A.1985; Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4. Journal of Virology 56:558–570
DrahosD. J., HendrixR. W.1982; Effect of bacteriophage lambda infection on synthesis of groEprotein and other Escherichia coliproteins. Journal of Bacteriology 149:1050–1063
HightowerL. E., SmithM. D.1978; Effects of canavanine on protein metabolism in Newcastle disease virus- infected chicken embryo cells. In Negative Strand Viruses and the Host Cell pp 395–405MahyB. W. J., BarryR. D. Edited by New York: Academic Press;
KaoH-T., NevinsJ. R.1983; Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection. Molecular and Cellular Biology 3:2058–2065
KennedyP. G. E., LathangueN. B., ChanW. L., ClementsG. B.1985; Cultured human neural cells accumulate a heat-shock protein during acute herpes virus infection. Neuroscience Letters 61:321–326
KhandjianE. W., TurlerH.1983; Simian virus 40 and polyoma virus induce synthesis of heat shock proteins in permissive cells. Molecular and Cellular Biology 3:1–8
KnipeD. M., BattersonW., NosalC., RoizmanB., BuchanA.1981; Molecular genetics of herpes simplex virus. VI. Characterization of a temperature-sensitive mutant defective in the expression of all early viral gene products. Journal of Virology 38:539–547
LiG. C., WerbZ.1982; Correlation between synthesis of heat shock proteins and development of thermotolerance in Chinese hamster fibroblasts. Proceedings of the National Academy of Sciences U.S.A.: 793218–3222
MacnabJ. C. M., OrrA., LathangueN. B.1985; Cellular proteins expressed in herpes simplex virus transformed cells also accumulate on herpes simplex virus infection. EMBO Journal 4:3223–3228
NotarianniE. L., PrestonC. M.1982; Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce immediate early polypeptides. Virology 123:113–122
PatelR., ChanW. L., KempL. M., LathangueN. B., LatchmanD. S.1986; Isolation of cDNA clones derived from a cellular gene transcriptionally induced by herpes simplex virus. Nucleic Acids Research 14:5629–5640
PelusoR. W., LambR. A., ChoppinP. W.1978; Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus or deprived of glucose. Proceedings of the National Academy of Sciences U.S.A.: 75477–480
PostL. E., MackemS., RozmanB.1981; Regulation of genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with agene promoters. Cell 24:555–565
PrestonC. M.1979a; Abnormal properties of an immediate early polypeptide in cells infected with the herpes simplex virus type 1 mutant tsK. Journal of Virology 32:357–369
PrestonC. M.1979b; Control of herpes simplex virus type 1 mRNA synthesis in cells infected with wild-type virus or the temperature-sensitive mutant tsK. Journal of Virology 29:275–284
PrestonV. G.1981; Fine-structure mapping of herpes simplex virus type 1 temperature-sensitive mutations within the short repeat region of the genome. Journal of Virology 39:150–161