%0 Journal Article %A Rodgers, Brian %A Borysiewicz, Leszek %A Mundin, Jane %A Graham, Shek %A Sissons, Patrick %T Immunoaffinity Purification of a 72K Early Antigen of Human Cytomegalovirus: Analysis of Humoral and Cell-mediated Immunity to the Purified Polypeptide %D 1987 %J Journal of General Virology, %V 68 %N 9 %P 2371-2378 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-68-9-2371 %K human|early antigens|T cells %K CMV %I Microbiology Society, %X Summary An early antigen of human cytomegalovirus (HCMV) was purified from infected cells as a 72K polypeptide by immunoaffinity chromatography using a monoclonal antibody. It was located in both nucleus and cytoplasm, and was non-glycosylated and undetectable on the surface of infected cells. Known seropositive subjects had antibody against the purified protein and it elicited proliferative T cell responses in 10 of 16 subjects. Five of 14 T cell lines established in response to the purified protein were predominantly CD8+ and of these two showed major histocompatibility complex- restricted cytotoxicity against HCMV-infected cells. This provides further evidence that antigens expressed at early times may be targets for the immune response during persistent HCMV infection. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-9-2371