Preparation and Properties of Immune-stimulating Complexes Containing Hepatitis B Virus Surface Antigen Free

Abstract

Summary

Immune-stimulating complexes (iscoms) have been prepared containing the major S gene products (HBsAg) of the hepatitis B virus genome. Immunization of B ALB/c mice with a single dose of hepatitis B iscoms in saline resulted in a high titre antibody response to HBsAg. In contrast, the original HBsAg preparation required an adjuvant to produce equivalent amounts of antibody. Analysis of sera from mice immunized with hepatitis B iscoms revealed antibodies directed against the major determinants of HBsAg. High secondary antibody responses were observed in immunized animals previously inoculated with a sub-immunogenic dose of HBsAg indicating that hepatitis B iscoms may represent a suitable immunogen for use in individuals in whom a course of immunization with currently licensed hepatitis B vaccines has failed to produce a significant anti-HBs response.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-9-2281
1987-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/9/JV0680092281.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-9-2281&mimeType=html&fmt=ahah

References

  1. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  2. Brown S. E., Howard C. R., Zuckerman A. J., Steward M. W. 1984; The determination of the affinity of antibodies to hepatitis B surface antigen. Journal of Immunological Methods 72:41–48
    [Google Scholar]
  3. Brown S. E., Stanley C., Howard C. R., Zuckerman A. J., Steward M. W. 1986; Antibody responses to recombinant and plasma derived hepatitis B vaccines. British Medical Journal 292:159–161
    [Google Scholar]
  4. Feuerhake A., Muller R., Lauchart W., Pichlmayr R., Schmidt F. W. 1984; HBV-vaccination in recipients of kidney allografts. Vaccine 2:255–256
    [Google Scholar]
  5. Hanatani M., Nishifuji K., Futai M., Tsuchiya T. 1984; Solubilization and reconstitution of membrane proteins of Escherichia coliusing alkanoyl-N-methylglucamides. Journal of Biochemistry 95:1349–1353
    [Google Scholar]
  6. Hildreth J. E. K. 1982; N-d-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry. Biochemical Journal 207:363–366
    [Google Scholar]
  7. Hilleman M. R., Buynak E. B., Mcaleer W. J., Mclean A. A., Provost P. J., Tytell A. A. 1982; Hepatitis A and hepatitis B vaccines. In Viral Hepatitis pp 385–397 Szmuness W., Alter H. J., Maynard J. E. Edited by Philadelphia: Franklin Institute Press;
    [Google Scholar]
  8. Howard C. R. 1986; The biology of hepadnaviruses. Journal of General Virology 67:1215–1235
    [Google Scholar]
  9. Howard C. R., Zuckerman A. J. 1984; New viral hepatitis vaccines. In Control of Virus Diseases pp 33–55 Kurstak E. Edited by New York: Marcel Dekker;
    [Google Scholar]
  10. Howard C. R., Skelly J., Tabor E., Gerety R. J., Kremastinou J., Zuckerman A. J. 1982; The development arid properties of alternative hepatitis B polypeptide vaccines. In Viral Hepatitis pp 411–423 Alter H., Maynard J., Szmuness W. Edited by Philadelphia: Franklin Institute Press;
    [Google Scholar]
  11. Howard C. R., Young P. R., Lee S., Dixon J., Zuckerman A. J. 1986; Hepatitis B surface antigen polypeptide micelles from antigen expressed in Saccharomyces cerevisiae . Journal of Virological Methods 14:25–35
    [Google Scholar]
  12. Jilg W., Schmidt M., Zoulek G., Lorbeer B., Wilske B., Deinhardt F. 1984; Clinical evaluation of a recombinant hepatitis B vaccine. Lancet ii:1174–1175
    [Google Scholar]
  13. Mcaleer W. J., Buynak E. B., Maigetler R. Z., Wampler D. E., Miller W. J., Hilleman M. R. 1984; Human hepatitis B vaccine from recombinant yeast-derived surface antigen. Nature; London: 307178–180
    [Google Scholar]
  14. Milich D. R., Thornton G. B., Neurath A. R., Kent S. B., Michel M-L., Tiollais P. 1985; Enhanced immunogenicity of the pre-S region of hepatitis B surface antigen. Science 228:1195–1198
    [Google Scholar]
  15. Morein B., Sundquist B., Hoglund S., Dalsgaard K., Osterhaus A. 1984; Iscom, a novel structure for antigenic presentation of membrane proteins from enveloped viruses. Nature; London: 308457–460
    [Google Scholar]
  16. Osterhaus A., Weijer K., Uytdenhaag F., Jarrett O., Sundquist B., Morein B. 1985; Induction of protective immune response in cats by vaccination with feline leukemia virus iscom. Journal of Immunology 135:591–596
    [Google Scholar]
  17. Sanchez Y., Ionescu-Matiu I., Sparrow J. T., Melnick J. L., Dreesman G. R. 1982; Immunogenicity of conjugates and micelles of synthetic hepatitis B surface antigen peptides. Intervirology 18:209–213
    [Google Scholar]
  18. Skelly J., Howard C. R., Zuckerman A. J. 1979; Analysis of hepatitis B surface antigen components solubilized with Triton X-100. Journal of General Virology 44:679–689
    [Google Scholar]
  19. Skelly J., Howard C. R., Zuckerman A. J. 1981; Hepatitis B polypeptide vaccine preparation in micelle form. Nature; London: 29051–54
    [Google Scholar]
  20. Stevens C., Alter H., Taylor P., Zang E., Harley E., Szmunees W. 1984; Hepatitis B vaccine in patients receiving hemodialysis: immunogenicity and efficacy. New England Journal of Medicine 311:496–501
    [Google Scholar]
  21. Szmuness W., Stevens C. E., Harley E. J., Zang E. A., Olesko W. R., William D. C., Sadovsky R., Morrison J. M., Kellner A. 1980; Demonstration of efficacy in a controlled clinical trial in high risk population in the United States. New England Journal of Medicine 303:833–841
    [Google Scholar]
  22. Szmuness W., Stevens C. E., Harley E. J., Zang E. A., Alter H. J., Taylor P. E., Devera A., Chen G.-T.-S., Kellser A. 1982; Hepatitis B vaccine in medical staff of hemodialysis units: efficacy and subtype crossprotection. New England Journal of Medicine 307:1481–1486
    [Google Scholar]
  23. Tabor E., Howard C. R., Skelly J., Goudeau A., Zuckerman A. J., Gerety R. J. 1982; Immunogenicity in chimpanzees of experimental hepatitis B vaccines prepared from intact hepatitis B virions. Journal of Medical Virology 10:65–74
    [Google Scholar]
  24. Valenzuela P., Medina A., Rutter W. J., Ammerer G., Hall B. D. 1982; Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature; London: 298347–350
    [Google Scholar]
  25. Wampler D. E., Lehman E. D., Boger I., McAleer W. J., Scolnick E. M. 1985; Multiple chemical forms of hepatitis B surface antigen produced in yeast. Proceedings of the National Academy of Sciences U.S.A.: 826830–6834
    [Google Scholar]
  26. Young P. R., Vaudin M., Dixon I., Zuckerman A. I. 1982; Preparation of hepatitis B polypeptide micelles from human carrier plasma. Journal of Virological Methods 4:177–185
    [Google Scholar]
  27. Zuckerman A. J. 1982; Primary hepatocellular carcinoma and hepatitis B virus. Transactions of the Royal Society of Tropical Medicine and Hygiene 76:711–718
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-9-2281
Loading
/content/journal/jgv/10.1099/0022-1317-68-9-2281
Loading

Data & Media loading...

Most cited Most Cited RSS feed