%0 Journal Article %A Howard, C. R. %A Sundquist, B. %A Allan, J. %A Brown, S. E. %A Chen, S-H. %A Morein, B. %T Preparation and Properties of Immune-stimulating Complexes Containing Hepatitis B Virus Surface Antigen %D 1987 %J Journal of General Virology, %V 68 %N 9 %P 2281-2289 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-68-9-2281 %K surface antigens %K hepatitis B virus %K immune-stimulating complexes %I Microbiology Society, %X Summary Immune-stimulating complexes (iscoms) have been prepared containing the major S gene products (HBsAg) of the hepatitis B virus genome. Immunization of B ALB/c mice with a single dose of hepatitis B iscoms in saline resulted in a high titre antibody response to HBsAg. In contrast, the original HBsAg preparation required an adjuvant to produce equivalent amounts of antibody. Analysis of sera from mice immunized with hepatitis B iscoms revealed antibodies directed against the major a determinants of HBsAg. High secondary antibody responses were observed in immunized animals previously inoculated with a sub-immunogenic dose of HBsAg indicating that hepatitis B iscoms may represent a suitable immunogen for use in individuals in whom a course of immunization with currently licensed hepatitis B vaccines has failed to produce a significant anti-HBs response. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-9-2281