%0 Journal Article %A Bapat, Ashok R. %A Han, Fu-Sheng %A Liu, Zongchao %A Zhou, Bing-Sen %A Cheng, Yung-Chi %T Studies on DNA Topoisomerases I and II in Herpes Simplex Virus Type 2-infected Cells %D 1987 %J Journal of General Virology, %V 68 %N 8 %P 2231-2237 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-68-8-2231 %K HSV-2 %K DNA polymerase %K topoisomerase %I Microbiology Society, %X Summary It has been suggested that herpes simplex virus (HSV) type 1 may induce a virus-specific DNA topoisomerase activity which copurifies with virus-induced DNA polymerase. We have examined DNA topoisomerase (TOPO) I and II activities in HSV-2-infected HeLa S3 cells. Both activities were partially purified using DEAE-cellulose, phosphocellulose and double-stranded DNA cellulose column chromatography. It was found that both activities could be separated from HSV-2-specific DNA polymerase. Throughout the purification TOPO I could be immunologically detected with a monoclonal antibody developed against human TOPO I. Regardless of the source, mock- or HSV-2-infected human cells, both types of topoisomerase were equally tolerant of 200 mm-KCl. There appeared to be no apparent heterogeneity of TOPO I in HeLa S3 cells through the course of the HSV-2 infection. We conclude that host cell topoisomerases are quite stable in HSV-2-infected HeLa S3 cells and that there is no evidence that HSV-2 is capable of inducing HSV-2-specific TOPO I and TOPO II activities. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-8-2231