1887

Abstract

SUMMARY

Adult athymic nude (BALB/c background) mice or inbred BALB/c mice were inoculated intraperitoneally with Hantaan virus (HV), and attempts were made to isolate the virus from brain, lung and spleen. Virus was isolated from the organs of BALB/c mice for only a short time after infection but was isolated from various organs of nude mice consistently for at least 84 days after infection. Viral antigen was also detected in various organs of nude mice for a long time after infection. The effects of adoptive transfer of immune serum or immune T cells from BALB/c to nude mice before or after virus inoculation were examined. Before transfer, the T cell fraction was treated with complement (C′) (group 1), anti-L3T4 + C′ (group 2), anti-Lyt1 .2 + C′ (group 3) or anti-Lyt2.2 + C′ (group 4). When transferred before virus inoculation to test the effects on protection against infection, immune serum and T cells of groups 1,2 and 4 were effective. When transferred after HV inoculation to test the effects on clearance of virus, group 1 was the most effective followed by group 2. These results suggest that humoral and cellular immunity both have roles in protection against HV infection, and that T cells possessing L3T4 Lyt2 markers on the cell-surface are especially important for elimination of infectious virus .

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1987-07-01
2024-03-29
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References

  1. Allan J. E., Doherty P. C. 1985; Immune T cells can protect or induce fatal neurological disease in murine lymphocytic choriomeningitis. Cellular Immunology 30:401–407
    [Google Scholar]
  2. Byrne J. A., Oldstone M. B. A. 1984; Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus: clearance of virus in vivo . Journal of Virology 51:682–686
    [Google Scholar]
  3. Cantor H., Boyse E. A. 1976; Regulation of cellular and humoral immune responses by T-cell subclasses. Cold Spring Harbor Symposia on Quantitative Biology 41:23–32
    [Google Scholar]
  4. Dialynas D. P., Wilde D. B., Marrack P., Pierres A., Wall K. A., Havran W., Otten G., Loken M. R., Pierres M., Kappler J., Fitch F. W. 1983a; Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunological Reviews 74:29–56
    [Google Scholar]
  5. Dialynas D. P., Quan Z. S., Wall K. A., Pierres A., Quintans J., Loken M. R., Pierres M., Fitch F. W. 1983b; Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5:similarity of L3T4 to the human LEU-3/T4 molecule. Journal of Immunology 131:2445–2451
    [Google Scholar]
  6. Ertl H. C. 1981; Adoptive transfer of delayed-type hypersensitivity to Sendai virus. I. Induction of two different subsets of T lymphocytes which differ in H-2 restriction as well as in the Lyt phenotype. Cellular Immunology 62:38–49
    [Google Scholar]
  7. Hirsch M. S., Zisman B., Allison A. C. 1970; Macrophages and age-dependent resistance to herpes simplex virus in mice. Journal of Immunology 104:1160–1165
    [Google Scholar]
  8. Hung T., Xia S. H., Shao T. X., Shou J. Y., Song G., Liao G. X. H., Ye W. W., Chu L. Y., Hang C. S. 1983; Morphological evidence for identifying the viruses of hemorrhagic fever with renal syndrome as candidate members of the Bunyaviridae family. Archives of Virology 78:137–144
    [Google Scholar]
  9. Hung T., Chou Z., Zhao T., Xia S., Hang C. 1985; Morphology and morphogenesis of viruses of hemorrhagic fever with renal syndrome (HFRS). Intervirology 23:97–108
    [Google Scholar]
  10. Jacoby R. o., Bhatt P. N., Schwartz A. 1980; Protection of mice from lethal flavi viral encephalitis by adoptive transfer of splenic cells from donors infected with live virus. Journal of Infectious Diseases 141:617–624
    [Google Scholar]
  11. Kai C., Tamura T., Fujiwara K. 1981; Effect of immune heterozygous spleen cell transfer on resistance to mouse hepatitis virus infection in nude mice. Microbiology and Immunology 25:1011–1018
    [Google Scholar]
  12. Kim G. R., Mckee K. T. Jr 1985; Pathogenesis of Hantaan virus infection in suckling mice; clinical, virologic, and serologic observations. American Journal of Tropical Medicine and Hygiene 34:388–395
    [Google Scholar]
  13. Kohl S., Tang J. P., Loo L. S. 1984; Antibody-dependent cellular cytotoxicity and natural killer cytotoxicity of peritoneal cells from nude mice to herpes simplex virus-infected cells. Microbiology and Immunology 28:439–449
    [Google Scholar]
  14. Kohl S., Thomas J. W., Loo L. S. 1986; Defective production of anti-herpes simplex virus antibody by neonatal mice. Reconstitution with Ia+ macrophages and T helper lymphocytes from non-immune adult syngeneic mice. Journal of Immunology 136:3038–3044
    [Google Scholar]
  15. Kurata T., Tsai T. F., Bauer S. P., Mccormick J. B. 1983; Immunofluorescence studies of disseminated Hantaan virus infection of suckling mice. Infection and Immunity 41:391–398
    [Google Scholar]
  16. Kurata T., Hondo R., Sato S., Oda A., Aoyama Y. 1984; Detection of viral antigens in formalin-fixed specimens by enzyme treatment. Annals of the New York Academy of Science 420:192–207
    [Google Scholar]
  17. Kyuwa S., Fujiwara K. 1984; Delayed-type hypersensitivity in mouse hepatitis virus infection in mice. Japanese Journal of Experimental Medicine 54:81–86
    [Google Scholar]
  18. Larsen H. s., Russell R. G., Rouse B. T. 1983; Recovery from lethal herpes simplex virus type 1 infection is mediated by cytotoxic T lymphocytes. Infection and Immunity 41:197–204
    [Google Scholar]
  19. Larsen H. s., Feng M. F., Horohov D. W., Moore R. N., Rouse B. T. 1984; Role of T-lymphocyte subsets in recovery from herpes simplex virus infection. Journal of Virology 50:56–59
    [Google Scholar]
  20. Ledbetter J. A., Rouse R. V., Micklem H. S., Herzenberg L. A. 1980; T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. Journal of Experimental Medicine 152:280–295
    [Google Scholar]
  21. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. Journal of Infectious Diseases 137:298–308
    [Google Scholar]
  22. Lee H. W., French G. R., Lee P. W., Baeck L. J., Tsuchiya K., Foulke R. S. 1981a; Observation on natural and laboratory infection of rodents with the etiologic agent of Korean hemorrhagic fever. American Journal of Tropical Medicine and Hygiene 30:477–482
    [Google Scholar]
  23. Lee H. W., Lee P. W., Beck L. J., Song C. K., Seong I. W. 1981b; Intraspecific transmission of Hantaan virus etiologic agent of Korean hemorrhagic fever in the rodent Apodemus agrarius . American Journal of Tropical Medicine and Hygiene 30:1106–1112
    [Google Scholar]
  24. Lee P. W., Amyx H. L., Gibbs C. J. Jr Gajdusek D. C., Lee H. W. 1981; Propagation of Korean hemorrhagic fever virus in laboratory rats. Infection and Immunity 31:334–338
    [Google Scholar]
  25. Mccormick J. B., Sasso D. R., Palmer E. R., Kiley M. P. 1982; Morphological identification of the agent of Korean haemorrhagic fever (Hantaan virus) as a member of the Bunyaviridae. Lancet i:765–768
    [Google Scholar]
  26. Mathieson B. J., Sharrow S. O., Campbell P. S., Asofsky R. 1979; An Lyt differentiated thymocyte subpopulation by flow microfluorometry. Nature; London: 277478–480
    [Google Scholar]
  27. Nagafuchi S., Oda H., Mori R., Taniguchi T. 1979; Mechanism of acquired resistance to herpes simplex virus infection as studied in nude mice. Journal of General Virology 44:715–723
    [Google Scholar]
  28. Nagafuchi S., Hayashida I., Higa K., Wada T., Mori R. 1982; Role of Lyt-1 positive immune T cells in recovery from herpes simplex virus infection in mice. Microbiology and Immunology 26:359–362
    [Google Scholar]
  29. Nakamura T., Yanagihara R, Gibbs C. J., Amyx H. L., Gajdusek D. C. 1985a; Differential susceptibility and resistance of immunocompetent and immunodeficient mice to fatal Hantaan virus infection. Archives of Virology 86:109–120
    [Google Scholar]
  30. Nakamura T., Yanagihara R., Gibbs C. J., Gajdusek D. C. 1985b; Immune spleen cell-mediated protection against fatal Hantaan virus infection in infant mice. Journal of Infectious Diseases 151:691–697
    [Google Scholar]
  31. Rager-Zisman B., Allison A. C. 1973; Effects of immunosuppression on coxsackie-B-3 virus infection in mice, and passive protection by circulating antibody. Journal of General Virology 19:339–351
    [Google Scholar]
  32. Schmaljohn C. S., Dalrymple J. M. 1983a; Analysis of Hantaan virus RNA: evidence for a new genus of bunyaviridae. Virology 131:482–491
    [Google Scholar]
  33. Schmaljohn C. S., Haisty S. E., Harrison S. A., Dalrymple J. M. 1983b; Characterization of Hantaan virions, the prototype virus of hemorrhagic fever with renal syndrome. Journal of Infectious Diseases 148:1005–1012
    [Google Scholar]
  34. Schmaljohn C. S., Hasty S. E., Dalrymple J. M., Leduc J. W., Lee H. W., Vaheri A., Tsai T. F., Regnery H. L., Goldgaber D., Lee P. W. 1985; Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science 227:1041–1044
    [Google Scholar]
  35. Stohlman S. A., Frelinger J. A., Weiner L. P. 1980; Resistance to fatal central nervous system disease by mouse hepatitis virus, strain JHM. Journal of Immunology 124:1733–1739
    [Google Scholar]
  36. Stohlman S. A., Matsushima G. K., Casteel N., Weiner L. P. 1986; In vivo effects of coronavirus-specific T cell clones: DTH inducer cells prevent a lethal infection but do not inhibit virus replication. Journal of Immunology 136:3052–3056
    [Google Scholar]
  37. Swain S. L. 1981; Significance of Lyt phenotypes :Lyt2 antibodies block activities of T cells that recognize class 1 major histocompatibility complex antigens regardless of their function. Proceedings of the National Academy of Sciences, U.S.A 78:7101–7105
    [Google Scholar]
  38. Swain S. L. 1983; T cell subsets and the recognition of MHC class. Immunological Reviews 74:129–142
    [Google Scholar]
  39. Tanishita O., Takahashi Y., Okuno Y., Yamanishi K., Takahashi M. 1984; Evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrome virus. Journal of Clinical Microbiology 20:1213–1215
    [Google Scholar]
  40. Tanishita O., Takahashi Y., Okuno Y., Tamura M., Asada H., Dantas J. R. Jr Yamanouchi T., Domae K., Kurata T., Yamanishi K. 1986; Persistent infection of rats with haemorrhagic fever with renal syndrome virus and their antibody responses. Journal of General Virology 67:2819–2824
    [Google Scholar]
  41. Tsai T. F., Mccormick J. B., Kurata T. 1982; Intracerebral inoculation of suckling mice with Hantaan virus. Lancet ii:503–504
    [Google Scholar]
  42. White J. D., Shirey F. G., French G. R., Hugging J. W., Brand O. M., Lee H. W. 1982; Hantaan virus, aetiological agent of Korean haemorrhagic fever has bunyaviridae-like morphology. Lancet i:768–771
    [Google Scholar]
  43. Wilde D. B., Marrack P., Kappler J., Dialynas D. P., Fitch F. W. 1983; Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5(anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. Journal of Immunology 131:2178–2183
    [Google Scholar]
  44. Yamanishi K., Dantas J. R. Jr Takahashi M., Yamanouchi T., Domae K., Kawamata J., Kurata T. 1983; Isolation of hemorrhagic fever with renal syndrome (HRFS) virus from a tumor specimen in a rat. Biken Journal 26:155–160
    [Google Scholar]
  45. Yamanishi K., Dantas J. R. Jr Takahashi M., Yamanouchi T., Domae K., Takahashi Y., Tanishita O. 1984; Antigenic differences between two viruses, isolated in Japan and Korea, that cause hemorrhagic fever with renal syndrome. Journal of Virology 52:231–237
    [Google Scholar]
  46. Yamanouchi T., Domae K., Tanishita O., Takahashi Y., Yamanishi K., Takahashi M., Kurata T. 1984; Experimental infection in newborn mice and rats by hemorrhagic fever with renal syndrome (HFRS) virus. Microbiology and Immunology 28:1345–1353
    [Google Scholar]
  47. Yanagihara R., Amyx H. L., Gajdusek D. C. 1985a; Experimental infection with Puumala virus, the etiologic agent of nephropathia epidemica, in bank voles (Clethrionomys glareolus). Journal of Virology 55:34–38
    [Google Scholar]
  48. Yanagihara R., Goldgaber D., Gajdusek D. C. 1985b; Propagation of nephropathia epidemica virus in Mongolian gerbils. Journal of Virology 53:973–975
    [Google Scholar]
  49. Zinkernagel R. M., Welsh R. M. 1976; H-2 compatibility requirement for virus-specific T cell-mediated effector functions in vivo. Specificity of T cells conferring antiviral protection against lymphocytic choriomeningitis virus is associated with H-2K and H-2D. Journal of Immunology 117:1495–1502
    [Google Scholar]
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