RT Journal Article SR Electronic(1) A1 Ghosh-Choudhury, Nandini A1 Graham, Alex A1 Ghosh, Hara P.YR 1987 T1 Herpes Simplex Virus Type 2 Glycoprotein Biogenesis: Effect of Monensin on Glycoprotein Maturation, Intracellular Transport and Virus Infectivity JF Journal of General Virology, VO 68 IS 7 SP 1939 OP 1949 DO https://doi.org/10.1099/0022-1317-68-7-1939 PB Microbiology Society, SN 1465-2099, AB SUMMARY The ionophore monensin inhibited the formation of herpes simplex virus type 2 (HSV-2) particles by about 30% but the yields of infectious particles were reduced to 5 % and 1 % for cell-associated and extracellular virus, respectively. The presence of monensin did not affect the processing of the two viral glycoproteins gB-2 and gG-2. However, two other glycoproteins, gC-2 and gD-2, were not processed to their fully mature forms in the monensin-treated cells and only the faster moving pgC-2 and pgD-2 were detected. The cell-associated virus particles contained the glycoproteins gB-2, gC-2, gD-2 and gG-2, whereas the extracellular virus particles contained only gG-2 glycoprotein. These results suggest that HSV-2 particles containing all the viral glycoproteins are transported via the Golgi apparatus to the cell surface but that virus particles containing only gG-2 may follow a different pathway for transport and release., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-7-1939