1887

Abstract

SUMMARY

We have used antisera raised against synthetic oligopeptides to characterize the protein products from herpes simplex virus type 1 gene U11. These antisera recognized predominantly polypeptides of apparent molecular weight 21000 and 22000, but also polypeptides of apparent molecular weight 17500, 15000, 14000 and 11000. Tryptic peptide fingerprint analysis confirmed that these polypeptides were all closely related. The 21000 and 22000 molecular weight polypeptides were shown to be DNA-binding proteins, and immune electron microscopy demonstrated their strong localization within nucleoli of infected cells.

Keyword(s): DNA-binding protein , HSV-1 and nucleolus
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1987-07-01
2024-04-24
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References

  1. Anderson C. W., Baum P. R., Gesteland R. F. 1973; Processing of adenovirus 2-induced protein. Journal of Virology 12:241–252
    [Google Scholar]
  2. Bayliss G. J., Marsden H. S., Hay J. 1975; Herpes simplex virus proteins: DNA-binding proteins in infected cells and in the virus structure. Virology 68:124–134
    [Google Scholar]
  3. Becker Y., Dym H., Sarov I. 1968; Herpes simplex virus DNA. Virology 36:184–192
    [Google Scholar]
  4. Bernhard W. 1969; A new staining procedure for electron microscopical cytology. Journal of Ultrastructure Research 27:250–265
    [Google Scholar]
  5. Brown S. M., Harland J. 1987; Three mutants of herpes simplex virus type 2: one lacking the genes US10, US11 and US 12 and two in which Rs has been extended by 6 kb to 0-91 map units with loss of Us sequences between 0-94 and the US/TRS junction. Journal of General Virology 68:1–18
    [Google Scholar]
  6. Brown S. M., Ritchie D. A., Subak-Sharpe J. H. 1973; Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. Journal of General Virology 18:329–346
    [Google Scholar]
  7. Chou J., Roizman B. 1986; The terminal ‘a’ sequence of the HSV genome contains the promoter of a gene located in the repeat sequences of the L component. Journal of Virology 57:629–637
    [Google Scholar]
  8. Dalziel R. G., Marsden H. S. 1984; Identification of two herpes simplex virus type 1-induced proteins (21K and 22K) which interact specifically with the a sequence of herpes simplex virus DNA. Journal of General Virology 65:1467–1475
    [Google Scholar]
  9. Dupuy-Coin A. M., Arnoult J., Bouteille M. 1978; Quantitative correlation of morphological alterations on the nucleus with functional events during in vitro infection of glial cells with herpes simplex hominis (HSV2). Journal of Ultrastructure Research 65:60–72
    [Google Scholar]
  10. Frame M. C., Mcgeoch D. J., Rixon F. J., Orr A. C., Marsden H. S. 1986; The 10K virion phosphoprotein encoded by gene Us9 from herpes simplex virus type 1. Virology 150:321–332
    [Google Scholar]
  11. Haarr L., Marsden H. S., Preston C. M., Smiley J. R., Summers W. C., Summers W. P. 1985; Utilization of internal AUG codons for initiation of protein synthesis directed by mRNA from normal and mutant genes encoding herpes simplex virus-specified thymidine kinase. Journal of Virology 56:512–519
    [Google Scholar]
  12. Henderson L. E., Oroszlan S., Konigsbeerg W. 1979; A micromethod for complete removal of dodecyl sulfate from proteins by ion-pair extraction. Analytical Biochemistry 93:153–157
    [Google Scholar]
  13. Hirs C. A. W. 1967; Performic acid oxidation. Methods in Enzymology 11:189–199
    [Google Scholar]
  14. Hugle B., Scheer U., Franke W. W. 1985a; Ribocharin: a nuclear Mr 40,000 protein specific to precursor particles of the large ribosomal subunit. Cell 41:615–627
    [Google Scholar]
  15. Hugle B., Hazan R., Scheer U., Franke W. W. 1985b; Localisation of ribosomal protein SI in the granular component of the interphase nucleolus and its distribution during mitosis. Journal of Cell Biology 100:873–886
    [Google Scholar]
  16. Jacob R. J., Roizman B. 1977; Anatomy of herpes simplex virus DNA. VIII. Properties of the replicating DNA. Journal of Virology 23:394–411
    [Google Scholar]
  17. Jacob R. J., Morse L. S., Roizman B. 1979; Anatomy of herpes simplex virus DNA. XII. Accumulation of head-to-tail concatemers in nuclei of infected cells and their role in the generation of the four isomeric arrangements of viral DNA. Journal of Virology 29:448–457
    [Google Scholar]
  18. Johnson P. A., Maclean C., Marsden H. S., Dalziel R. G., Everett R. D. 1986; The product of gene US11 of herpes simplex virus type 1 is expressed as a true late gene. Journal of General Virology 67:871–883
    [Google Scholar]
  19. Jordan E. G. 1984; Nucleolar nomenclature. Journal of Cell Science 67:217–220
    [Google Scholar]
  20. Locker H., Frenkel N. 1979; Bam 1, Kpn 1, and Sal 1 restriction enzyme maps of the DNAs of HSV strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNA. Journal of Virology 32:429–441
    [Google Scholar]
  21. Longnecker R., Roizman B. 1986; Generation of an inverting HSV 1 mutant lacking the L-S junction ‘a’ sequences, an origin of DNA synthesis, and several genes including those specifying glycoprotein E and the α47 gene. Journal of Virology 58:583–591
    [Google Scholar]
  22. Lonsdale D. M., Brown S. M., Subak-Sharpe J. H., Warren K. G., Koprowski H. 1979; The polypeptide and the DNA restriction enzyme profiles of spontaneous isolates of herpes simplex virus type 1 from explants of human trigeminal, superior cervical and vagus ganglia. Journal of General Virology 43:151–171
    [Google Scholar]
  23. Mcgeoch D. J., Dolan A., Donald S., Rixon F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
    [Google Scholar]
  24. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  25. Marsden H. s., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  26. Marsden H. S., Stow N. D., Preston V. P., Timbury M. C., Wilkie N. M. 1978; Physical mapping of herpes simplex virus-induced polypeptides. Journal of Virology 28:624–642
    [Google Scholar]
  27. Mocarski E. S., Roizman B. 1982; Structure and role of the herpes simplex virus DNA termini in inversion, circularisation and generation of virion DNA. Cell 31:89–97
    [Google Scholar]
  28. Nil S., Morgan C., Rose H. M., Hsu K. C. 1968; Electron microscopy of herpes simplex virus. IV. Studies with ferritin-conjugated antibodies. Journal of Virology 2:1172–1184
    [Google Scholar]
  29. Purifoy D. J. M., Powell K. L. 1976; DNA-binding proteins induced by herpes simplex virus type 2 in HEp-2 cells. Journal of Virology 19:717–731
    [Google Scholar]
  30. Puvion-Dutilleul F., Pichard E., Sheldrick P., Arratric F., Puvion E. 1985; Appearance of host-specific nucleolar proteins in intranuclear ‘dense bodies’ following herpes simplex infection. European Journal of Cell Biology 39:458–468
    [Google Scholar]
  31. Randall R. E., Dinwoodie N. 1986; Intranuclear localization of herpes simplex virus immediate-early and delayed-early proteins: evidence that ICP4 is associated with progeny virus DNA. Journal of General Virology 67:2163–2177
    [Google Scholar]
  32. Rixon F. J., Mcgeoch D. J. 1984; A 3′ co-terminal family of mRNAs from herpes simplex virus type 1 short region: two overlapping reading frames encode unrelated polypeptides one of which has a highly re-iterated amino acid sequence. Nucleic Acids Research 12:2473–2487
    [Google Scholar]
  33. Rixon F. J., Atkinson M. A., Hay J. 1983; Intranuclear distribution of herpes simplex virus type 2 DNA synthesis: examination by light and electron microscopy. Journal of General Virology 64:2087–2092
    [Google Scholar]
  34. Roizman B. 1979; The structure and isomerisation of HSV genomes. Cell 16:481–494
    [Google Scholar]
  35. Schwartz J., Roizman B. 1969; Similarities and differences in the development of laboratory strains and freshly isolated strains of herpes simplex virus in HEp-2 cells: electron microscopy. Journal of Virology 4:879–889
    [Google Scholar]
  36. Sheldrick P., Berthelot N. 1974; Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harbor Symposia on Quantitative Biology 39:667–678
    [Google Scholar]
  37. Sirtori C., Bosisio-Bestetti M. 1967; Nucleolar changes in KB cells infected with herpes simplex virus. Cancer Research 27:367–376
    [Google Scholar]
  38. Sommerville J. 1986; Nucleolar structure and ribosome biogenesis. Trends in Biochemical Sciences 11:438–442
    [Google Scholar]
  39. Stow N. D., Mcmonagle E. C., Davison A. J. 1983; Fragments from both termini of the herpes simplex virus type 1 genome contain signals required for the encapsidation of viral DNA. Nucleic Acids Research 11:8205–8220
    [Google Scholar]
  40. Timbury M. C. 1971; Temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 16:147–151
    [Google Scholar]
  41. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  42. Umene K. 1986; Conversion of a fraction of the unique sequence to part of the inverted repeats in the S component of the herpes simplex virus type 1 genome. Journal of General Virology 67:1035–1048
    [Google Scholar]
  43. Varmuza S. L., Smiley J. R. 1985; Signals for site-specific cleavage of HSV DNA: maturation involves two separate cleavage events at sites distal to the recognition sequences. Cell 41:793–802
    [Google Scholar]
  44. Wagner E. K., Roizman B. 1969; Ribonucleic acid synthesis in cells infected with herpes simplex virus. I. Patterns of ribonucleic acid synthesis in productively infected cells. Journal of Virology 4:36–46
    [Google Scholar]
  45. Wagner M. J., Summers W. C. 1978; Structure of the joint region and termini of the DNA of herpes simplex virus type 1. Journal of Virology 27:374–387
    [Google Scholar]
  46. Watson R. J., Vande Woude G. F. 1982; DNA sequence of an immediate-early gene (IE mRNA-5) of herpes simplex virus type 1. Nucleic Acids Research 10:979–991
    [Google Scholar]
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