1887

Abstract

SUMMARY

Antisera to the 32000 (32K) capsid and 16500 (16·5K) major non-capsid proteins were used for immunological analyses of extracts from maize stripe virus (MStpV)-infected maize () plants and from inoculative , the MStpV planthopper vector. The 32K protein was easily detected in extracts of both MStpV-infected plants and inoculative by ELISA and by immunological analysis of Western blots. In a time course study, no 32K protein was detected in until 8 days after the beginning of a 5 day acquisition access period on MStpV-infected plants. The percentage of MStpV-positive increased with time indicating multiplication of MStpV in . The 32K protein was detected only in individual that also transmitted MStpV to plant hosts. The 16·5K protein was also detected in MStpV-infected plant hosts but not in extracts of groups or of individual MStpV-inoculative translation of MStpV virion RNAs in rabbit reticulocyte lysates showed that both the 32K and 16·5K proteins were present in the translation products. The ready detection of the MStpV-coded 32K protein in both plant and insects and detection of the 16·5K protein in only plant hosts is discussed.

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1987-07-01
2021-10-20
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References

  1. Ammar E. D., Gingery R. E., Nault L. R. 1985; Two types of inclusions in maize infected with maize stripe virus. Phytopathology 75:84–89
    [Google Scholar]
  2. Burnette W. N. 1981; “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated Protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  3. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  4. Dougherty W. G., Hiebert E. 1980a; Translation of potyvirus RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins as products of tobacco etch virus RNA translation and cylindrical inclusion protein as a product of the potyvirus genome. Virology 104:174–182
    [Google Scholar]
  5. Dougherty W. G., Hiebert E. 1980b; Translation of potyvirus RNA in a rabbit reticulocyte lysate: cell-free translation strategy and a genetic map of the potyviral genome. Virology 104:183–194
    [Google Scholar]
  6. Falk B. w., Tsai J. H. 1983; Assay for maize stripe virus-infected plants by using antiserum produced to a purified noncapsid protein. Phytopathology 73:1259–1262
    [Google Scholar]
  7. Falk B. w., Tsai J. H. 1984; Identification of single-and double-stranded RNAs associated with maize stripe virus. Phytopathology 74:909–915
    [Google Scholar]
  8. Falk B. W., Morales F. J., Tsai J. H., Niessen A. I. 1987; Serological and biochemical analysis of the capsid and major noncapsid proteins of maize stripe virus, rice hoja blanca virus and Echinochloa hoja blanca virus. Phytopathology 77:196–201
    [Google Scholar]
  9. Gingery R. E. 1987; The rice stripe virus group. In The Filamentous Plant Viruses Milne R. G. Edited by New York: Plenum Press; in press
    [Google Scholar]
  10. Gingery R. E., Nault L. R., Bradfute O. E. 1981; Maize stripe virus: characteristics of a member of a new virus class. Virology 112:99–108
    [Google Scholar]
  11. Gingery R. E., Nault L. R., Yamashita S. 1983; Relationship between maize stripe virus and rice stripe virus. Journal of General Virology 64:1765–1770
    [Google Scholar]
  12. Gliedman J. B., Smith J. F., Brown D. T. 1975; Morphogenesis of Sindbis virus in cultured Aedes albopictus cells. Journal of Virology 16:913–926
    [Google Scholar]
  13. Gordon D. T. 1984; Comments of the chairperson of the international working group on maize virus diseases. Maize Virus Diseases Newsletter 1:1–11
    [Google Scholar]
  14. Hibino H., Usugi T., Omura T., Tsuchizaski T., Shohara K., Iwasaki M. 1985; Rice grassy stunt virus: a planthopper-bome circular filament. Phytopathology 75:894–899
    [Google Scholar]
  15. Jones P., Carpenter J. M., Autrey L. J. c. 1984; Investigations into the association between a non-capsid protein and virus infection in maize. Maize Virus Diseases Newsletter 1:52–53
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  17. Liu H. Y., Kimura I., Black L. M. 1975; Specific infectivity of different wound tumor virus isolates. Virology 52:320–326
    [Google Scholar]
  18. Morales F. J., Niessen A. I. 1983; Association of spiral filamentous virus-like particles with rice hoja blanca. Phytopathology 73:971–974
    [Google Scholar]
  19. Morrisey J. H. 1981; Silver stain for protein in polyacrylamide gels. A modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117:307–310
    [Google Scholar]
  20. Nuss D. L., Summers D. 1984; Variant ds-RNAs associated with transmission-defective isolates of wound tumor virus represent terminally conserved remnants of genome segments. Virology 133:276–286
    [Google Scholar]
  21. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  22. Reddy D. V. R., Black L. M. 1977; Isolation and replication of mutant populations of wound tumor virions lacking certain genome segments. Virology 80:336–346
    [Google Scholar]
  23. Scheefers-Borchel U., Scheefers H., Edwards J., Brown D. T. 1981; Sindbis virus maturation in cultured mosquito cells is sensitive to actinomycin D. Virology 110:292–301
    [Google Scholar]
  24. Toriyama S. 1982; Characterization of rice stripe virus: a heavy component carrying infectivity. Journal of General Virology 61:187–195
    [Google Scholar]
  25. Toriyama S. 1986; An RNA-dependent RNA polymerase associated with the filamentous nucleoproteins of rice stripe virus. Journal of General Virology 67:1247–1255
    [Google Scholar]
  26. Tsai J. H., Zitter T. A. 1982; Characteristics of maize stripe virus transmission by the com delphacid. Journal of Economic Entomology 75:397–400
    [Google Scholar]
  27. White J. L., Brakke M. K. 1983; Protein changes in wheat infected with wheat streak mosaic virus and in barley infected with barley stripe mosaic virus. Physiological Plant Pathology 22:87–100
    [Google Scholar]
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