1887

Abstract

Summary

The 32000 mol. wt. non-structural protein (P3) of alfalfa mosaic virus (A1MV) has previously been shown to accumulate in the cell wall fraction of tobacco leaves infected with AlMV. We now report the ultrastructural location of this protein. P3 was visualized immunocytochemically in the middle lamella of the walls of those parenchymal or epidermal cells which had just been reached by the infection front and in which viral multiplication had just begun. P3 was not found when AlMV had accumulated to high levels in infected cells. These findings support the concept that P3 is involved in the spread of viral infection from cell to cell, i.e. is the transport factor of AlMV.

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/content/journal/jgv/10.1099/0022-1317-68-6-1779
1987-06-01
2022-01-29
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References

  1. Atabekov J. G., Dorokhov Y. L. 1984; Plant virus-specific transport function and resistance of plants to viruses. Advances in Virus Research 29:313–364
    [Google Scholar]
  2. Barker R. F., Jarvis N. P., Thompson D. V., Loesch-Fries L. S., Hall T. C. 1983; Complete nucleotide sequence of alfalfa mosaic virus RNA 3. Nucleic Acids Research 11:2881–2891
    [Google Scholar]
  3. Berna A., Briand J.-P., Stussi-Garaud C., Godefroy-Colburn T. 1986; Kinetics of accumulation of the three non-structural proteins of alfalfa mosaic virus in tobacco plants. Journal of General Virology 67:1135–1147
    [Google Scholar]
  4. French R., Janda M., Ahlquist P. 1986; Bacterial gene inserted in an engineered RNA virus: efficient expression in a monocotyledonous plant cell. Science 231:1294–1297
    [Google Scholar]
  5. Garaud J.-C., Doffoel M., Stock C., Grenier J.-F. 1982; . Are “G” cells the only source of gastrin in the human antrum? Biology of the Cell 44:165–176
    [Google Scholar]
  6. Godefroy-Colburn T., Gagey M.-J., Berna A., Stussi-Garaud C. 1986; A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. Journal of General Virology 67:2233–2239
    [Google Scholar]
  7. Kiberstis P. A., Loesch-Fries L. S., Hall T. C. 1981; Viral protein synthesis in barley protoplasts infected with native and fractionated brome mosaic virus RNA. Virology 112:804–808
    [Google Scholar]
  8. Langenberg W. G. 1986; Virus protein association with cylindrical inclusions of two viruses that infect wheat. Journal of General Virology 67:1161–1168
    [Google Scholar]
  9. Leonard D. A., Zaitlin M. 1982; A temperature-sensitive strain of tobacco mosaic virus defective in cell-to-cell movement generates an altered viral-coded protein. Virology 117:416–424
    [Google Scholar]
  10. Nassuth A., Bol J. F. 1983; Altered balance of the synthesis of plus and minus strand RNAs induced by RNA 1 and 2 of alfalfa mosaic virus in absence of RNA 3. Virology 124:75–85
    [Google Scholar]
  11. Ohno T., Takamatsu T., Meshi T., Okada Y., Nishiguchi M., KiHo Y. 1983; Single amino acid substitution in 30K protein of TMV defective in virus transport function. Virology 131:255–258
    [Google Scholar]
  12. Pinck L., Hirth L. 1972; The replicative RNA and the viral RNA synthesis rate in tobacco infected with alfalfa mosaic virus. Virology 49:413–425
    [Google Scholar]
  13. Ravelonandro M., Pinck M., Pinck L. 1984; Complete nucleotide sequence of RNA 3 from alfalfa mosaic virus, Strain S. Biochimie 66:395–402
    [Google Scholar]
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