@article{mbs:/content/journal/jgv/10.1099/0022-1317-68-6-1773, author = "Brown, Lynn M. and Wood, K. Roger", title = "Translation of Clover Yellow Mosaic Virus RNA in Pea Mesophyll Protoplasts and Rabbit Reticulocyte Lysate", journal= "Journal of General Virology", year = "1987", volume = "68", number = "6", pages = "1773-1778", doi = "https://doi.org/10.1099/0022-1317-68-6-1773", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-6-1773", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "CYMV", keywords = "protoplasts", keywords = "translation", abstract = "Summary RNA from clover yellow mosaic virus, a potexvirus, was translated in rabbit reticulocyte lysates, and in vitro translation products were compared with virus-specific proteins produced in vivo in pea mesophyll protoplasts inoculated with viral RNA. One major polypeptide with an M r of 182000 (182K) was produced in vitro, and two were produced in vivo, with M r 182K and 22K. The 182K product was thought to be the same in vitro as in vivo; the 22K product was shown by Western blotting to be the virus coat protein.", }