1887

Abstract

Summary

RNA from clover yellow mosaic virus, a potexvirus, was translated in rabbit reticulocyte lysates, and translation products were compared with virus-specific proteins produced in pea mesophyll protoplasts inoculated with viral RNA. One major polypeptide with an of 182000 (182K) was produced , and two were produced , with 182K and 22K. The 182K product was thought to be the same as ; the 22K product was shown by Western blotting to be the virus coat protein.

Keyword(s): CYMV , protoplasts and translation
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-6-1773
1987-06-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/6/JV0680061773.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-6-1773&mimeType=html&fmt=ahah

References

  1. Abouhaidar M. G. 1983; The structure of the 5′ and 3′ ends of clover yellow mosaic virus RNA. Canadian Journal of Microbiology 29:151–156
    [Google Scholar]
  2. Atabekov J. G., Morozov S. Y. 1979; Translation of plant virus messenger RNAs. Advances in Virus Research 25:1–87
    [Google Scholar]
  3. Aviv H., Leder P. 1972; Purification of biologically active globin mRNA by chromatography on oligothymidylic acid-cellulose. Proceedings of the National Academy of Sciences U.S.A: 691408–1412
    [Google Scholar]
  4. Bancroft J. B., Abouhaidar M. G., Erickson J. W. 1979; The assembly of clover yellow mosaic virus and its protein. Virology 98:121–130
    [Google Scholar]
  5. Bendena W. G., Mackie G. A. 1986; Translation strategies in potexviruses: products encoded by clover yellow mosaic virus, foxtail mosaic virus and viola mottle virus RNAs in vitro. Virology 153:220–229
    [Google Scholar]
  6. Bendena W. G., Abouhaidar M. G., Mackie G. A. 1985; Synthesis in vitro of the coat protein of papaya mosaic virus. Virology 140:257–268
    [Google Scholar]
  7. Bendena W. G., Bancroft J. B., Mackie G. A. 1987; Molecular cloning of clover yellow mosaic virus RNA: identification of coat protein coding sequences in vivo and in vitro. Virology in press
    [Google Scholar]
  8. Bos L. 1973; Clover yellow mosaic virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses111
    [Google Scholar]
  9. Dougherty W. G., Hiebert E. 1985; Genome structure and gene expression of plant RNA viruses. In Molecular Plant Virology 2 pp. 23–81 Davies J. W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  10. Forster R. L. S., Guilford P. J., Faulds D. V. 1987; Characterization of the coat protein subgenomic RNA of white clover mosaic virus. Journal of General Virology 68:181–190
    [Google Scholar]
  11. Franssen H., Moerman M., Rezelman G., Goldbach R. 1984; Evidence that 32,000 dalton protein encoded by bottom component RNA of cowpea mosaic virus is a proteolytic processing enzyme. Journal of Virology 50:183–190
    [Google Scholar]
  12. Guilford P. J., Forster R. L. S. 1986; Detection of polyadenylated subgenomic RNAs in leaves infected with the potexvirus daphne virus X. Journal of General Virology 67:83–90
    [Google Scholar]
  13. Hill J. H., Shepherd R. J. 1972; Molecular weights of plants virus coat proteins by PAGE. Virology 47:817–822
    [Google Scholar]
  14. Jen G., Thach R. E. 1982; Inhibition of host translation in encephalomyocarditis virus-infected L cells. A novel mechanism. Journal of Virology 43:250–261
    [Google Scholar]
  15. Koenig R. 1971; Nucleic acids in the potato virus X group and in some other plant viruses: comparison of the molecular weights by electrophoresis in acrylamide-agarose composite gels. Journal of General Virology 10:111–114
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  17. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  18. Mackie G. A., Bancroft J. B. 1986; The longer RNA species in narcissus mosaic virus encodes all viral functions. Virology 153:215–219
    [Google Scholar]
  19. Maule A. J., Boulton M. I., Wood K. R. 1980a; An improved method for the infection of cucumber leaf protoplasts with cucumber mosaic virus. Phytopathologische Zeitschrift 97:118–126
    [Google Scholar]
  20. Maule A. J., Boulton M. I., Edmunds C., Wood K. R. 1980b; Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. Journal of General Virology 47:199–203
    [Google Scholar]
  21. Oldfield S. J., Wood K. R. 1984; Translation of potato virus X RNA in vivo and in vitro. Abstracts, Sixth International Congress of Virology224
    [Google Scholar]
  22. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  23. Purcifull D. E., Shepherd R. J. 1964; Preparation of the protein fragments of several rod-shaped plant viruses and their use in agar gel diffusion tests. Phytopathology 54:1102–1108
    [Google Scholar]
  24. Rao D. V., Hiruki C. 1978; Infection of cowpea mesophyll protoplasts with clover yellow mosaic virus. Journal of General Virology 38:303–311
    [Google Scholar]
  25. Short M. N., Davies J. W. 1983; Narcissus mosaic virus: a potex virus with an encapsidated subgenomic mRNA for coat protein. Bioscience Reports 3:837–846
    [Google Scholar]
  26. Valverde R. A., Dodds J. A., Heick J. A. 1986; Double-stranded ribonucleic acid from plants infected with viruses having elongated particles and undivided genomes. Phytopathology 76:459–465
    [Google Scholar]
  27. Watanabe Y., Emori Y., Ooshika I., Meshi T., Ohno T., Okada Y. 1984; Synthesis of TMV-specific RNAs and proteins at the early stage of infection in tobacco protoplasts: transient expression of the 30K protein and its mRNA. Virology 133:18–24
    [Google Scholar]
  28. Wodnar-Filipowicz A., Skrzeczkowski L. J., Filipowicz W. 1980; Translation of potato virus X RNA into high molecular weight proteins. FEBS Letters 109:151–155
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-6-1773
Loading
/content/journal/jgv/10.1099/0022-1317-68-6-1773
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error