@article{mbs:/content/journal/jgv/10.1099/0022-1317-68-6-1767, author = "Kurath, Gael and Leong, J. C.", title = "Transcription in vitro of Infectious Haematopoietic Necrosis Virus, a Fish Rhabdovirus", journal= "Journal of General Virology", year = "1987", volume = "68", number = "6", pages = "1767-1771", doi = "https://doi.org/10.1099/0022-1317-68-6-1767", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-6-1767", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "transcription in vitro", keywords = "IHNV", keywords = "fish rhabdovirus polymerase", abstract = "Summary Transcription in vitro by the RNA polymerase of infectious haematopoietic necrosis virus (IHNV), a salmonid rhabdovirus, was investigated using different reaction conditions to maximize RNA synthesis. The use of HEPES buffer rather than Tris buffer, and the addition of S-adenosyl-l-methionine to the reactions resulted in a sixfold increase in RNA synthetic activity to 6400 pmol UMP incorporated/mg viral protein/hour. The RNA transcripts produced in this system contained polyadenylated species which co-migrated with IHNV mRNA species 2, 3, 4 and 5 from IHNV- infected cells. The transcripts were shown to be functional mRNA species by their ability to direct the synthesis of viral proteins in vitro. ", }