1887

Abstract

Summary

Transcription by the RNA polymerase of infectious haematopoietic necrosis virus (IHNV), a salmonid rhabdovirus, was investigated using different reaction conditions to maximize RNA synthesis. The use of HEPES buffer rather than Tris buffer, and the addition of -adenosyl--methionine to the reactions resulted in a sixfold increase in RNA synthetic activity to 6400 pmol UMP incorporated/mg viral protein/hour. The RNA transcripts produced in this system contained polyadenylated species which co-migrated with IHNV mRNA species 2, 3, 4 and 5 from IHNV- infected cells. The transcripts were shown to be functional mRNA species by their ability to direct the synthesis of viral proteins

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/content/journal/jgv/10.1099/0022-1317-68-6-1767
1987-06-01
2021-10-22
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