@article{mbs:/content/journal/jgv/10.1099/0022-1317-68-6-1761, author = "Dressler, G. R. and Rock, D. L. and Fraser, N. W.", title = "Latent Herpes Simplex Virus Type 1 DNA Is Not Extensively Methylated in vivo", journal= "Journal of General Virology", year = "1987", volume = "68", number = "6", pages = "1761-1765", doi = "https://doi.org/10.1099/0022-1317-68-6-1761", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-6-1761", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "latent infection", keywords = "DNA methylation", keywords = "HSV-1", abstract = "Summary The methylation pattern of herpes simplex virus type 1 (HSV-1) DNA, present in the central nervous system of latently infected mice, was examined by digestion of the DNA with methylation-sensitive restriction endonucleases and Southern blot hybridization. Using the enzymes SmaI, XmaI, SalI and SacII, the data indicate no extensive methylation of latent HSV-1 DNA in vivo. Thus, extensive methylation of the viral genome is not a necessary condition for, or a consequence of maintaining, the latent state in vivo. ", }