1887

Abstract

Summary

We describe here a persistently infected cell system with unique properties. Cells infected with human parainfluenza virus type 3 (PF3) at high multiplicities of infection showed little or no cytopathic effects (cell fusion). Unlike other paramyxovirus persistent infections that require a long development time, the majority of the cells survived the initial infection and formed persistently infected cell cultures that were immediately available for study. In addition, unlike other paramyxovirus persistent infections, the PF3 system described here produced high levels of infectious virus and did not undergo periodic crises. Although cells persistently infected with PF3 contained large amounts of the cleaved, active form of the viral fusion protein, F, the persistently infected cells did not fuse with each other. However, they did fuse with uninfected cells within minutes of cell-to-cell contact. Other persistent paramyxovirus infections do not have this property. Fusion occurred with all cells tested, including red blood cells, and was not dependent on protein synthesis. The unique fusion properties of these PF3 persistently infected cells make this an interesting system for the study of mechanisms of viral fusion and mechanisms of inhibition of viral fusion.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-6-1737
1987-06-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/6/JV0680061737.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-6-1737&mimeType=html&fmt=ahah

References

  1. Elango N., Coligan J. E., Jambou R. C., Venkatesan S. 1986; Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of the purified protein. Journal of Virology 57:481–489
    [Google Scholar]
  2. Frank A. L., Taber I. H., Wells C. R., Wells J. M., Glezen W. P., Paredes A. 1981; Patterns of shedding of myxoviruses and paramyxoviruses in children. Journal of Infectious Diseases 144:433–441
    [Google Scholar]
  3. Galinski M. S., Mink M. A., Lambert D. M., Wechsler S. L., Pons M. W. 1986a; Molecular cloning and sequencing of the human parainfluenza 3 virus RNA encoding the nucleocapsid protein. Virology 149:139–151
    [Google Scholar]
  4. Galinski M. S., Mink M. A., Lambert D. M., Wechsler S. L., Pons M. W. 1986b; Sequence homology of nucleocapsid proteins within the Paramyxovirus family. In The Biology of Negative Strand Viruses Mahy B. W. J., Kolakofsky D. Edited by Amsterdam: Elsevier/North-Holland; in press
    [Google Scholar]
  5. Galinski M. S., Mink M. A., Lambert D. M., Wechsler S. L., Pons M. W. 1986c; Sequence of the human parainfluenza virus 3 RNA encoding the phosphoprotein and the C protein. Virology 155:46–60
    [Google Scholar]
  6. Goswami K. K. A., Cameron K. R., Russell W. C., Lange L. S., Mitchell D. N. 1984; Evidence for the persistence of paramyxoviruses in human bone marrows. Journal of General Virology 65:1881–1888
    [Google Scholar]
  7. Gross P. A., Green R. H., Curnen M. G. M. 1973; Persistent infections with parainfluenza type 3 virus in man. American Review of Respiratory Disease 108:894–898
    [Google Scholar]
  8. Mills B. G., Singer F. R., Weiner L. P., Holst P. A. 1981; Immunohistological demonstration of respiratory synctial virus antigens in Paget disease of the bone. Proceedings of the National Academy of Science U.S.A.: 781209–1213
    [Google Scholar]
  9. Morgan E. M., Rapp F. 1977; Measles virus and its associated diseases. Bacteriological Reviews 41:636–666
    [Google Scholar]
  10. Muchmore H. G., Parkinson A. J., Humphries J. F., Scott E. N., Mcintosh D. A., Scott L. V., Cooney M. K., Miles J. A. R. 1981; Persistent parainfluenza virus shedding during isolation at the South Pole. Nature; London: 289187–189
    [Google Scholar]
  11. Rima B. K., Martin S. J. 1976; Persistent infection of tissue culture cells BY RNA viruses. Medical Microbiology and Immunology 162:89–118
    [Google Scholar]
  12. Roux L., Holland J. J. 1979; Role of defective interfering particles of Sendai virus in persistent infections. Virology 93:91–103
    [Google Scholar]
  13. Rous L., Waldvogel F. A. 1981; Establishment of Sendai virus persistent infection: biochemical analysis of the early phase of standard plus defective interfering virus infection of BHK cells. Virology 112:400–410
    [Google Scholar]
  14. Rustigian R. 1966a; Persistent infection of cells in culture by measles virus. I. Development and characteristics of HeLa sublines persistently infected with complete virus. Journal of Bacteriology 92:1794–1804
    [Google Scholar]
  15. Rustigian R. 1966b; Persistent infection of cells in culture by measles virus. II. Effect of measles antibody on persistently infected HeLa sublines and recovery of a HeLa clonal line persistently infected with incomplete virus. Journal of Bacteriology 92:1805–1811
    [Google Scholar]
  16. Sanchez A, Benerjee A. K. 1985; Studies on human parainfluenza virus 3: characterization of the structural proteins and in vitro synthesized proteins coded by mRNAs isolated from infected cells. Virology 143:45–54
    [Google Scholar]
  17. Sanchez A., Banerjee A. K., Furuichi Y., Richardson M. A. 1986; Conserved structures among the nucleocapsid proteins of the paramyxoviridae: complete nucleotide sequence of the human parainfluenza virus type 3 NP mRNA. Virology 152:171–180
    [Google Scholar]
  18. Spriggs M. K., Olmsted R. A., Venkatesan S., Coligan J. E., Collins P. L. 1986; Fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage activation site and comparison with other paramyxoviruses. Virology 152:241–251
    [Google Scholar]
  19. Storey D. G., Dimock K., Kang C. Y. 1984; Structural characterization of virion proteins and genomic RNA of human parainfluenza virus 3. Journal of Virology 52:761–766
    [Google Scholar]
  20. Tyeryar F. J. 1983; Report of a workshop on respiratory syncytial virus and parainfluenza viruses. Journal of Infectious Diseases 148:588–598
    [Google Scholar]
  21. Wechsler S. L., Fields B. N. 1978; Intracellular synthesis of measles virus-specified polypeptides. Journal of Virology 25:285–297
    [Google Scholar]
  22. Wechsler S. L., Meissner H. C. 1981; Measles and SSPE viruses: similarities and differences. Progress in Medical Virology 28:64–95
    [Google Scholar]
  23. Wechsler S. L., Rustigian R., Stallcup K. C., Byers K. B., Winston S. H., Fields B. N. 1979; Measles virus- specified polypeptide synthesis in two persistently infected HeLa cell lines. Journal of Virology 31:677–684
    [Google Scholar]
  24. Wechsler S. L., Lambert D. M., Galinski M. S., Pons M. W. 1985a; Intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides. Journal of Virology 54:661–664
    [Google Scholar]
  25. Wechsler S. L., Lambert D. M., Galinski M. S., Heineke B. E., Pons M. W. 1985b; Human parainfluenza virus 3: purification and characterization of subviral components, viral proteins and viral RNA. Virus Research 3:339–351
    [Google Scholar]
  26. Wechsler S., Lambert D. M., Galinski M., Heineke B. E., Lambert A. L., Mink M., Rochovansky O. M., Pons M. W. 1985c; A simple method for increased recovery of purified paramyxovirus. Journal of Virological Methods 12:179–182
    [Google Scholar]
  27. Welliver R., Wong D. T., Choi T., Ogra P. L. 1982; Natural history of parainfluenza virus infection in childhood. Journal of Pediatrics 101:180–187
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-68-6-1737
Loading
/content/journal/jgv/10.1099/0022-1317-68-6-1737
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error