Transmembrane Cation Movements during Infection of by Bacteriophage LL-H Free

Abstract

Summary

Phage LL-H-induced cation (K, Na, Mg, Ca, Cd) movements in bacteria have been studied. The effects of the m.o.i. and external cation concentration have been quantified. LL-H-induced effluxes showed cation specificity: K but practically no Mg was lost during LL-H infection at low and moderate m.o.i. (up to about 100). Simultaneously to K efflux, divalent cation influxes were observed. These were dependent on the m.o.i. and on concentrations of external divalent cations and were concomitant with phage DNA transport, as concluded from the timing of the first phage-promoted biochemical changes in host cell metabolism and from electron microscopical observations. Host energy was not mobilized with phage-induced divalent cation influx. Several features of divalent cation influxes support the view that divalent cations have to be cotransported into the cell as counterions of LL-H DNA. Phage DNA associated with divalent cations may be the basic feature of the divalent cation dependence of LL-H infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-6-1525
1987-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/6/JV0680061525.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-6-1525&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Alatossava T. 1982; Factors affecting in vitro DNA ejection of the Lactobacillus lactis bacteriophage LL-H. Journal of General Virology 59:173–175
    [Google Scholar]
  3. Alatossava T., Pyhtilä M. J. 1980; Characterization of a new Lactobacillus lactis bacteriophage. IRCS Medical Science 8:297–298
    [Google Scholar]
  4. Alatossava T., Juvonen T., Huhtinen R. -L. 1983; Effect of cadmium on the infection of Lactobacillus lactis by bacteriophage LL-H. Journal of General Virology 64:1623–1627
    [Google Scholar]
  5. Filali Maltouf A., Labedan B. 1983; Host cell metabolic energy is not required for injection of bacteriophage T5 DNA. Journal of Bacteriology 153:124–133
    [Google Scholar]
  6. Furukawa H., Kuroiwa T., Mizushima S. 1983; DNA injection during bacteriophage T4 infection of Escherichia coli . Journal of Bacteriology 154:938–945
    [Google Scholar]
  7. Grinius L. 1980; Nucleic acid transport driven by ion gradient across cell membrane. FEBS letters 113:1–10
    [Google Scholar]
  8. Impraim C. C., Foster K. A., Micklem K. J., Pasternak C. A. 1980; Nature of virally-mediated changes in membrane permeability to small molecules. Biochemical Journal 186:847–860
    [Google Scholar]
  9. Karnik S. S., Gopinathan K. P. 1980; Possible involvement of a calcium stimulated ATP-hydrolyzing activity associated with Mycobacteriophage 13 in the DNA injection process. Journal of Virology 33:969–975
    [Google Scholar]
  10. Keweloh H., Barker E. P. 1984a; Increased permeability and subsequent resealing of the host cell membrane early after infection of Escherichia coli with bacteriophage T1. Journal of Bacteriology 160:354–359
    [Google Scholar]
  11. Keweloh H., Barker E. P. 1984b; Permeability changes in the cytoplasmic membrane of Escherichia coli K-12 early after infection with bacteriophage T1. Journal of Bacteriology 160:347–353
    [Google Scholar]
  12. Kuhn A., Kellenberger E. 1985; Productive phage infection can occur in Escherichia coli with reduced internal levels of the major cations. Journal of Bacteriology 163:906–912
    [Google Scholar]
  13. Kuhn A., Jötte H., Kellenberger E. 1983; Involvement of the bacterial groM gene product in bacteriophage T7 reproduction. II. A reduced level of ion concentrations causes the blockage of T7 maturation in K-12-M cells. Journal of Virology 47:540–552
    [Google Scholar]
  14. Landry E. F., Zsigray R. M. 1980; Effects OF calcium ON the lytic cycle OF Bacillus subtilis phage 41c. Journal of General Virology 51:125–135
    [Google Scholar]
  15. Letellier L., Labedan B. 1984; Involvement of envelope-bound calcium in the transient depolarization of the Escherichia coli cytoplasmic membrane induced by bacteriophage T4 and T5 adsorption. Journal of Bacteriology 157:789–794
    [Google Scholar]
  16. Micklem K. J., Nyaruwe A., Alder G. M., Pasternak C. A. 1984; The effect of Ca2+ on virus-cell fusion and permeability changes. Cell Calcium 5:537–550
    [Google Scholar]
  17. Moncany M. 1982 Déterminations des conditions intracellulaires chez Escherichia coli. Conséquences biologiques de leur modification Thése d’Etat Université Paris VII:
    [Google Scholar]
  18. Nagaraja V., Gopinathan K. P. 1980; Requirement for calcium ions in mycobacteriophage 13 DNA injection and propagation. Archives of Microbiology 124:249–254
    [Google Scholar]
  19. Pasternak C. A., Bashford C. L. 1985; Effect of Ca2+ and other divalent cations on pore-formation by viruses, toxins, complement and other agents. Studia biophysica 110:113–122
    [Google Scholar]
  20. Ponta H., Altendorf K. -H., Schweiger M., Hirsch-Kauffmann M., Pfenning-Yeh M. -L., Herrlich P. 1976; E. coli membranes become permeable to ions following T7-virus-infection. Molecular and General Genetics 149:145–150
    [Google Scholar]
  21. Reed P. W., Lardy H. A. 1972; A23187: a divalent cation ionophore. Journal of Biological Chemistry 247:6970–6977
    [Google Scholar]
  22. Rose D. M., Polnaszek C. F., Bryant R. G. 1982; 25Mg-NMR investigations of the magnesium ion-DNA interaction. Biopolymers 21:653–664
    [Google Scholar]
  23. Rountree P. M. 1955; The role of divalent cations in the multiplication of staphylococcal bacteriophages. Journal of General Microbiology 12:275–287
    [Google Scholar]
  24. Sarimo S. S., Aaltonen L. 1978; DNA, RNA and protein synthesis in ϕLL55-infected Lctobacillus lactis . Archives of Microbiology 116:191–195
    [Google Scholar]
  25. Silver S., Levine E., Spielman P. M. 1968; Cation fluxes and permeability changes accompanying bacteriophage infection of Escherichia coli . Journal of Virology 2:763–771
    [Google Scholar]
  26. Snipes W., Cupp J., Sands J. A., Keith A., Davis A. 1974; Calcium requirement for assembly of the lipid-containing bacteriophage PM2. Biochimica et biophysica acta 339:311–322
    [Google Scholar]
  27. Steensma H. Y., Blok J. 1979; Effect of calcium ions on the infection of Bacillus subtilis by bacteriophage SF6. Journal of General Virology 42:305–314
    [Google Scholar]
  28. Wagner E. F., Ponta H., Schweiger M. 1980; Development of Escherichia coli virus T1. The role of the proton motive force. Journal of Biological Chemistry 255:534–539
    [Google Scholar]
  29. Watanabe K., Takesue S. 1972; The requirement for calcium in infection with Lactobacillus phage. Journal of General Virology 17:19–30
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-6-1525
Loading
/content/journal/jgv/10.1099/0022-1317-68-6-1525
Loading

Data & Media loading...

Most cited Most Cited RSS feed