Long virus-like particles were found in the apical region of the calyx in the ichneumonid wasp . Replication began in foci of intranuclear inclusion bodies where long, 20 nm diameter electron-dense cylinders were produced. These putative nucleocapsids were released into the cytoplasm after nuclear disintegration and became enveloped in areas containing many membrane-bound vesicles. Mature particles appeared in large vacuoles and were released by cell lysis into the calyx lumen. The mature particles were enveloped, 65 nm in diameter with a 20 nm diameter nucleocapsid and a length of at least 1 µm with a long, narrow (20 nm diameter) end structure. This is the first report of a virus with such a morphology replicating in the family Ichneumonidae.


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