1887

Abstract

Summary

An outbreak of poliomyelitis in Finland resulted in the widespread circulation of wild-type 3 poliovirus strains that had antigenic properties distinct from the strains used to produce the attenuated and inactivated vaccines. Considerable variation was observed in the ability of broadly reacting monoclonal antibodies directed against type 3 poliovirus to neutralize the 54 strains examined. Sequential isolates from several persons showed an antigenic drift with these monoclonal antibodies and selected human sera. In addition, some faecal specimens were found to contain more than one antigenic variant. Primer extension sequencing of genomic RNAs of three plaque- purified antigenic variants isolated from one patient showed base substitutions in the region coding for the major antigenic site of poliovirus type 3. The resulting difference in the amino acid sequence in the virion protein VP1 could explain the differences observed in the neutralization of these strains by the monoclonal antibodies. Whether the observed changes in the antigenic characteristics of the sequential isolates represent true antigenic drift under immunological pressure or whether the emergence of the new variants is based on other modes of selection during replication is not known.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-5-1373
1987-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/5/JV0680051373.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-5-1373&mimeType=html&fmt=ahah

References

  1. Almond J. W., Stanway G., Cann A. J., Westrop G., Evans D. M. A., Ferguson M., Minor P. D., Spitz M., Schild G. c. 1985; New poliovirus vaccines: a molecular approach. Vaccine 2:177–183
    [Google Scholar]
  2. Böttiger M. 1971; Neutralization capacity of poliovirus type 3 antibodies tested against 5 different intratypic variants of poliovirus type 3. Archiv für die gesamte Virusforschung 35:231–255
    [Google Scholar]
  3. Evans D. M. A., Minor P. D., Schild G. C., Almond J. W. 1983; Critical role of an eight amino acid sequence of VP1 in neutralization of poliovirus type 3. Nature; London: 304459–462
    [Google Scholar]
  4. Evans D. M. A., Dunn G., Minor P. D., Schild G.C., Cann A. J., Stanway G., Almond J. W., Currey K., Maizel J. V. 1985; A single nucleotide change in the 5′ non-coding region of the genome of the Sabin type 3 poliovaccine is associated with increased neurovirulence. Nature; London: 314548–550
    [Google Scholar]
  5. Ferguson M., Minor P. D., Magrath D. I., Ql Yi-Hua, Spitz M., Schild G. C. 1984; Neutralization epitopes on poliovirus type 3 particles: an analysis using monoclonal antibodies. Journal of General Virology 65:197–201
    [Google Scholar]
  6. Ferguson M., Evans D. M. A., Magrath D. I., Minor P. D., Almond J. W., Schild G. C. 1985; Induction of broadly reactive, type-specific neutralizing antibody to poliovirus type 3 by synthetic peptides. Virology 143:505–515
    [Google Scholar]
  7. Hovi T., Cantell K., Huovilainen A., Kinnunen E., Kuronen T., Lapinleimu K., Pöyry T., Roivainen M., Salama N., Stenvik M., Silander A., Thoden C.-J., Salminen S., Weckström P. 1986; Outbreak of paralytic poliomyelitis in Finland: widespread circulation of antigenically altered poliovirus type 3 in a vaccinated population. Lancet i:1427–1432
    [Google Scholar]
  8. Icenogle J. p., Minor P. D., Ferguson M., Hogle J. M. 1986; Modulation of humoral response to a 12-amino acid site on the poliovirion. Journal of Virology 60:297–301
    [Google Scholar]
  9. Magrath D. I., Evans D. M. A., Ferguson M., Schild G. C., Minor P. D., Horaud F., Crainic R., Stenvik M., Hovi T. 1986; Antigenic and molecular properties of type 3 poliovirus responsible for an outbreak of poliomyelitis in a vaccinated population. Journal of General Virology 67:899–905
    [Google Scholar]
  10. Minor P. D., Evans D. M. A., Ferguson M., Schild G. C., Westrop G., Almond J. W. 1985; Principal and subsidiary antigenic sites of VP1 involved in the neutralization of poliovirus type 3. Journal of General Virology 66:1159–1165
    [Google Scholar]
  11. Minor P. D., John A., Ferguson M., Icenogle J. P. 1986; Antigenic and molecular evolution of the vaccine strain of type 3 poliovirus during the period of excretion by a primary vaccinee. Journal of General Virology 67:693–706
    [Google Scholar]
  12. Nottay B. K., Kew O. M., Hatch M. H., Heyward J. p., Obueskl J. F. 1981; Molecular variation of type 1 vaccine related and wild polioviruses during replication in humans. Virology 108:405–423
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-5-1373
Loading
/content/journal/jgv/10.1099/0022-1317-68-5-1373
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error