Infection of L929 cells by Newcastle disease virus (NDV) did not induce cell fusion, whereas addition of anti-L929 cell antiserum to the culture medium did result in cell fusion. When the antiserum was added to the culture medium of NDV-infected cells at the start of incubation, polykaryocytes appeared after 18 h post-infection (p.i.). On the other hand, when the antiserum was added to the culture medium at 18 h p.i., no polykaryocytes appeared, suggesting that the presence of the antiserum at the stage of NDV replication was necessary for the antiserum-induced cell fusion. Since anti-F polypeptide antiserum inhibited the cell fusion, functional F protein was essential for the antiserum-induced cell fusion. All the anti-L929 cell xenogenic sera tested were able to induce the cell fusion, whereas anti-L929 cell allogenic sera did not induce cell fusion. Anti-MCA cell serum also induced the cell fusion. Little replication of NDV was observed in the presence of anti-L929 cell serum and synthesis of virus-specific polypeptides, especially of M protein, was suppressed. However, there was no difference in expression of virus-specific glycoproteins on the surface membranes between cells treated with normal rabbit serum and those treated with anti-L929 cell serum. On the basis of the above results, the mechanism of induction of cell fusion by anti-host cell serum is discussed.


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  1. Ito Y., Tsurudome M., Hishiyama M. 1985; Persistence of mumps virus in mouse L929 cells. Microbiology and Immunology 29:847–857
    [Google Scholar]
  2. Storey D. G., Kang C. Y. 1985; Vesicular stomatitis virus-induced cells fuse when the intracellular pool of functional M protein is reduced in the presence of G protein. Journal of Virology 53:374–383
    [Google Scholar]
  3. Yamamoto F., Fujioka H., Iinuma M., Takano M., Maeno K., Nagai Y., Ito Y. 1984; Enhancement of Newcastle disease virus-induced fusion of mouse L cells by sodium vanadate. Microbiology and Immunology 28:75–83
    [Google Scholar]
  4. Yoshida T., Nagai Y., Maeno K., Iinuma M., Hamaguchi M., Matsumoto T., Nagayoshi S., Hoshino M. 1979; Studies on the role of M protein in virus assembly using a ts mutant of HVJ (Sendai virus). Virology 92:139–154
    [Google Scholar]

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