Nicotiana clevelandii plants doubly infected with potato leafroll luteovirus (PLRV) and potato Y potyvirus (PVY) developed more severe symptoms than plants infected with either virus alone. Compared with singly infected plants, the PLRV concentration was increased up to eightfold by the double infection and the proportion of leaf parenchyma protoplasts that could be stained with fluorescent antibody to PLRV was increased from 0·2 to 1·4%. The concentration of PVY was unaffected by the double infection. In potato plants, in contrast, double infection with PVY did not increase PLRV concentration and none of the parenchyma protoplasts obtained from singly or doubly infected leaves could be stained with fluorescent antibody to PLRV. PLRV seems restricted to phloem tissue in potato but also invades a few parenchyma cells in N. clevelandii, a process that is accentuated in plants also infected with PVY.
AraiK.,
DoiY.,
YoraK.,
AsuyamaH.1969; Electron microscopy of the potato leafroll virus in leaves of three kinds of host plants and the partial purification of the virus. Annals of the Phytopathological Society of Japan 35:10–15
AtabekovI. G.,
TalianskyM. E.,
DrampyanA. H.,
KaplanI. B.,
TupkaI. E.1984; Systemic infection by a phloem-restricted virus in parenchyma cells in a mixed infection. Biologicheskie Nauk 10:28–31 In Russian
BarkerH.,
HarrisonB. D.1986; Restricted distribution of potato leafroll virus antigen in resistant potato genotypes and its effect on transmission of the virus by aphids. Annals of Applied Biology 109:595–604
CarrR. J.,
KimK. S.1983; Evidence that bean golden mosaic virus invades non-phloem tissue in double infections with tobacco mosaic virus. Journal of General Virology 64:2489–2492
KuboS.,
TakanamiY.1979; Infection of tobacco mesophyll protoplasts with tobacco necrotic dwarf virus, a phloem-limited virus. Journal of General Virology 42:387–398
KuboS.,
HarrisonB. D.,
RobinsonD. J.,
MayoM. A.1975; Tobacco rattle virus in tobacco mesophyll protoplasts : infection and virus multiplication. Journal of General Virology 27:293–304
WeidemannH. L.,
CasperR.1982; Immunhistologische Untersuchungen uber das Vorkommen von Kartoffelblattrollvirus in Knolle und Spross der Kartoffelpflanze. Potato Research 25:99–106