1887

Abstract

SUMMARY

Normal guinea-pig serum (GPS) lacking detectable antiviral antibody efficiently neutralized the infectivity of influenza B virus grown in chick embryos or MDCK cells. The inhibitor was heat-labile and sensitive to trypsin digestion. This -like inhibitor required Ca and the complement components C1 and C4 for its activity. In contrast, GPS did not inactivate influenza A virus. Influenza B virus from which the neuraminidase activity of the spikes on the viral envelope had been eliminated by trypsin digestion was also inactivated to a level comparable to untreated virus. Complement component C1 alone bound directly to influenza B virus and inhibited its haemagglutinin activity. We suggest that the like inhibitor in GPS is a component of the classical complement pathway which is triggered by the protein moiety of influenza B virus haemagglutinin, leading to virus neutralization.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-4-1135
1987-04-01
2021-09-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/4/JV0680041135.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-4-1135&mimeType=html&fmt=ahah

References

  1. Ananthanaryan R., Paniker C. J. K. 160 Non-specific inhibitors of influenza viruses in normal sera. Bulletin of the World Health Organization 22:409–420
    [Google Scholar]
  2. Beebe D. p., Schriber R. D., Cooper N. R. 1983; Neutralization of influenza virus by normal human sera: mechanisms involving antibody and complement. Journal of Immunology 130:1317–1322
    [Google Scholar]
  3. Chu C. M. 1951; The action of normal mouse serum on influenza virus. Journal of General Microbiology 5:739–757
    [Google Scholar]
  4. Cooper N. R. 1979; Humoral immunity to viruses. In Comprehensive Virology 15 pp 123–164 Fraenkel-Conrat H., Wagner R. R. Edited by New York & London: Plenum Press;
    [Google Scholar]
  5. Cooper N. R. 1984; The role of the complement system in host defense against virus diseases. In Concepts in Viral Pathogenesis pp 20–25 Notkins A. L., Oldstone M. B. A. Edited by New York, Berlin, Heidelberg & Tokyo: Springer-Verlag;
    [Google Scholar]
  6. Fearon D. T., Austen K. f. 1977; Activation of the alternative complement pathway due to resistance of zymosan-bound amplification convertase to endogenous regulatory mechanisms. Immunology 74:1683–1687
    [Google Scholar]
  7. Francis T. Jr 1947; Dissociation of hemagglutinating and antibody measuring capacities of influenza virus. Journal of Experimental Medicine 85:1–7
    [Google Scholar]
  8. Gigli I., Porter R. R., Sim P. B. 1976; The unactivated form of the first component of human complement, Cl. Biochemical Journal 157:541–548
    [Google Scholar]
  9. Hawkes R., Niday E., Gordon J. 1982; A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119:142–147
    [Google Scholar]
  10. Inai S., Nagaki K., Ebisu S., Kato K., Kotani S., Misaki A. 1976; Activation of the alternative complement pathway by water-insoluble glucans of streptococcus mutants: the relation between their chemical structures and activating potencies. Journal of Immunology 117:1256–1260
    [Google Scholar]
  11. Krizanova O., Rathova V. 1969; Serum inhibitors of myxoviruses. Current Topics in Microbiology and Immunology 47:125–151
    [Google Scholar]
  12. Maeno K., Kilbourne E. D. 1970; Developmental sequence and intracellular site of synthesis of three structural protein antigens of influenza A2 virus. Journal of Virology 5:153–164
    [Google Scholar]
  13. Shibata M., Maeno K., Tsurumi T., Aoki H., Nishiyama Y., Ito Y., Isomura S., Suzuki S. 1982a; Role of viral glycoprotein in haemolysis by influenza B virus. Journal of General Virology 59:183–186
    [Google Scholar]
  14. Shibata M., Maeno K., Isomura S., Tsurumi T., Aoki H., Suzuki S. 1982b; Plaque formation by influenza B virus in a porcine kidney cell line. Microbiology and Immunology 26:441–444
    [Google Scholar]
  15. Shimojo H., Sugiura A., Akao J., Enomoto C. 1958; Studies of a non-specific hemagglutination inhibitor of influenza A (Asian) 57 virus. Bulletin of the Institute of Public Health 7:219–224
    [Google Scholar]
  16. Sissons J. G. P. 1984; Antibody- and complement-dependent lysis of virus-infected cells. In Concepts in Viral Pathogenesis pp 39–45 Notkins A. L., Oldstone M. B. A. Edited by New York, Berlin, Heidelberg & Tokyo: Springer-Verlag;
    [Google Scholar]
  17. Sugiura A., Shimojo H., Enomoto C. 1961; Studies of the non-specific hemagglutination inhibitor against influenza A2 virus (gamma-inhibitor). II. Physicochemical properties of the gamma-inhibitor. Japanese Journal of Experimental Medicine 31:159–168
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-4-1135
Loading
/content/journal/jgv/10.1099/0022-1317-68-4-1135
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error