RT Journal Article SR Electronic(1) A1 Milhaud, P. G. A1 Silhol, M. A1 Salehzada, T. A1 Lebleu, B.YR 1987 T1 Requirement for Endocytosis of Poly(rI).Poly(rC) to Generate Toxicity on Interferon-treated LM Cells JF Journal of General Virology, VO 68 IS 4 SP 1125 OP 1134 DO https://doi.org/10.1099/0022-1317-68-4-1125 PB Microbiology Society, SN 1465-2099, AB SUMMARY Poly(rI). poly(rC) induces a lytic reaction in interferon-treated mouse LM cells. We have attempted to determine whether the intracellular penetration of poly(rI). poly(rC) is a prerequisite for cell lysis and to gain some insight into the pathway followed. We found that poly(rI). poly(rC) coupled to Sepharose beads was unable to generate lysis of interferon-treated cells whereas the cells underwent lysis after microinjection of poly(rI).poly(rC). Some inhibitors of endocytosis were found to inhibit the development of the lytic reaction. Lysosomotropic amines or a low temperature (19 °C) blocked endocytosis of poly(rI). poly(rC) but did not prevent its uptake. The internalization of poly(rI). poly(rC) was energy-dependent and was blocked when sodium azide and 2-deoxyglucose were added simultaneously. We conclude that poly(rI). poly(rC) is internalized and reaches an acidic compartment before triggering the lytic reaction in the cell., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-4-1125