1887

Abstract

Summary

Vaccinia virus cores prepared can be uncoated by incubation with extracts of cells infected with vaccinia virus, as determined by the conversion of the genome to DNase susceptibility. This uncoating activity had all the characteristics of the corresponding activity and of the agent responsible for non-genetic reactivation. Thus, it was not induced by heat-inactivated virus, nor was it produced in the presence of inhibitors of RNA or protein synthesis. The uncoating protein induced by cowpox virus will uncoat vaccinia virus cores. The uncoating protein was concentrated from infected cell extracts by ultrafiltration and purified by gel filtration and ion-exchange and affinity chromatography. It was characterized as a trypsin-like protease with a mol. wt. of 23050. Cores treated with the purified uncoating protein had an altered sedimentation rate but no differences between treated and untreated cores were detected by electron microscopy or polyacrylamide gel electrophoresis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-4-1021
1987-04-01
2022-06-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/4/JV0680041021.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-4-1021&mimeType=html&fmt=ahah

References

  1. Abel P. 1963; Reactivation of heated vaccinia virusin vitro. Zeitschrift fur Vererbungslehre 94:249–252
    [Google Scholar]
  2. Bennet T. p. 1967; Membrane filtration for detecting proteins in the presence of interfering substances. Nature; London: 2131131–1132
    [Google Scholar]
  3. Berry G. P., Dedrick H. M. 1936; A method for changing the virus of rabbit fibroma (Shope) into that of infectious myxomatosis. Journal of Bacteriology 31:50–51
    [Google Scholar]
  4. Buchmeier N. A., Gee S. R., Murphy F. A., Rawls W. E. 1979; Abortive replication of vaccinia virus in activated rabbit macrophages. Infection and Immunity 26:328–338
    [Google Scholar]
  5. Cairns J. 1960; The initiation of vaccinia virus infection. Virology 11:603–623
    [Google Scholar]
  6. Coleman P. L., Latham H. G.Jr Shaw E. N. 1976; Some sensitive methods for the assay for trypsin-like enzymes. Methods in Enzymology 45: part B 12–26
    [Google Scholar]
  7. Dales S. 1963; Uptake and development of vaccinia virus in strain L cells followed with labelled viral DNA. Journal of Cell Biology 18:51–72
    [Google Scholar]
  8. Dales S. 1965; Relation between penetration of vaccinia virus, release of viral DNA and initiation of genetic functions. In Perspectives in Virology 4 pp 47–71 Pollard M. Edited by New York: Harper & Row;
    [Google Scholar]
  9. Dales S., Kajiora R. 1964; The cycle of multiplication of vaccinia virus in Earle’s strain L cells. I. Uptake and penetration. Virology 24:278–294
    [Google Scholar]
  10. Dales S., Mosbach E. 1968; Vaccinia as a model for membrane biogenesis. Virology 35:564–583
    [Google Scholar]
  11. Grimley P. M., Rosenblum E. N., Mims S. J., Moss B. 1970; Interruption by rifampin of an early stage in vaccinia virus morphogenesis : accumulation of membranes which are precursors of virus envelopes. Journal of Virology 6:519–533
    [Google Scholar]
  12. Holowczak J. A. 1972; Uncoating of poxviruses. I. Detection and characterisation of subviral particles in the uncoating processes. Virology 50:216–232
    [Google Scholar]
  13. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18
    [Google Scholar]
  14. Joklik W. K. 1964a; The intracellular uncoating of poxvirus DNA. I. The fate of radioactively labelled rabbitpox virus. Journal of Molecular Biology 8:263–276
    [Google Scholar]
  15. Joklik W. K. 1964b; The intracellular uncoating of poxvirus DNA. II. The molecular basis of the uncoating process. Journal of Molecular Biology 8:277–288
    [Google Scholar]
  16. Joklik W. K., Woodroofe G. M., Holmes J. H., Fenner F. 1960a; The reactivation of poxviruses Demonstration of the phenomenon and techniques of assay. Virology 11:168–183
    [Google Scholar]
  17. Joklik W. K., Holmes I. H., Briggs M. J. 1960b; The reactivation of poxviruses. III. Properties of reactivable particles. Virology 11:202–218
    [Google Scholar]
  18. Kates J., Beeson J. 1970; Vaccinia core RNA synthesis. Journal of Molecular Biology 50:1–18
    [Google Scholar]
  19. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurements with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  20. Magee W. E., Miller O. v. 1968; Initiation of vaccinia virus infection in actinomycin D pre-treated cells. Journal of Virology 2:678–685
    [Google Scholar]
  21. Moss B., Filler R. 1970; Irreversible effects of cycloheximide during the early period of vaccinia virus replication. Journal of Virology 5:99–108
    [Google Scholar]
  22. Nowakowski M., Bauer W., Kates J. 1978; Characterisation of a DNA-binding phosphoprotein from vaccinia virus replication complex. Virology 86:217–225
    [Google Scholar]
  23. Pedley S., Cooper R. J. 1984; Inhibition of HeLa cell RNA synthesis following infection with vaccinia virus. Journal of General Virology 65:1687–1697
    [Google Scholar]
  24. Pogo B. G. T., Katz J. R., Dales S. 1975; Biogenesis of poxviruses: synthesis and phosphorylation of a basic protein associated with the DNA. Virology 64:531–543
    [Google Scholar]
  25. Sarov I., Joklik W. K. 1972; Characterisation of intermediates in the uncoating of vaccinia virus DNA. Virology 50:593–602
    [Google Scholar]
  26. Soloski M. J., Holowczak J. A. 1981; Characterisation of supercoiled nucleoprotein complexes released from detergent-treated vaccinia virions. Journal of Virology 37:770–783
    [Google Scholar]
  27. Soloski M. J., Esteban M., Holowczak J. A. 1978; DNA binding proteins in the cytoplasm of vaccinia virus- infected mouse L cells. Journal of Virology 25:263–273
    [Google Scholar]
  28. Stern W., Dales S. 1976; Biogenesis of vaccinia: relationship of the envelope to virus assembly. Virology 75:242–255
    [Google Scholar]
  29. Steven F. S., Griffin M. M. 1981; Evidence for exchange of inhibitors which bind to the active site of trypsin EC-3.4.21.4. Displacement of one inhibitor with a competitive inhibitor. Biochimica et biophysica acta 662:190–195
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-4-1021
Loading
/content/journal/jgv/10.1099/0022-1317-68-4-1021
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error