@article{mbs:/content/journal/jgv/10.1099/0022-1317-68-3-937, author = "Graham, F. L.", title = "Growth of 293 Cells in Suspension Culture", journal= "Journal of General Virology", year = "1987", volume = "68", number = "3", pages = "937-940", doi = "https://doi.org/10.1099/0022-1317-68-3-937", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-3-937", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "cell line", keywords = "permissive", keywords = "adenovirus", abstract = "Summary A subline of 293 cells able to grow in suspension culture has been developed by passage of 293 cells through nude mice. This new line, designated 293N3S, grows with a doubling time of approximately 30 h, continues to express adenovirus 5 early region 1 (E1) antigens, and remains permissive for adenovirus 5 host range mutants defective in E1 functions.", }