1887

Abstract

Summary

The polyhedrin gene region of the nuclear polyhedrosis virus (AcMNPV) morphology mutant M29 has been characterized by genetic and physical techniques. Recombination analysis of mutants M29 and AcM5polyl demonstrated that wild-type polyhedrin recombinants could be obtained and that the DNA restriction patterns of the recombinant viruses were identical to wild-type AcMNPV DNA. Marker rescue experiments using the wild-type AcMNPV RI I fragment indicated that the morphology mutation responsible for the M29 phenotype was located in the 0·0 to 5·9% region of the genome. Direct DNA sequencing of the HI F fragment from M29 showed a single point mutation at position 253 of the polyhedrin gene. This mutation caused a substitution of phenylalanine for leucine at amino acid 84 of the M29 polyhedrin protein. These results indicated the necessity of amino acid conservation in the polyhedrin amino acid sequence for proper folding and assembly of the polypeptide into occlusion bodies.

Keyword(s): AcMNPV , baculovirus and polyhedrin
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/content/journal/jgv/10.1099/0022-1317-68-3-901
1987-03-01
2024-04-24
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References

  1. Brown M., Faulkner P. 1978; Plaque assay of nuclear polyhedrosis viruses in cell culture. Applied and Environmental Microbiology 36:31–35
    [Google Scholar]
  2. Brown M., Faulkner P., Cochran M. A., Chung K. L. 1980; Characterization of two morphology mutants of Autographa californica nuclear polyhedrosis virus with large cuboidal inclusion bodies. Journal of General Virology 50:309–316
    [Google Scholar]
  3. Carstens E. B. 1982; Mapping the mutation site of an Autographa californica nuclear polyhedrosis virus polyhedron morphology mutant. Journal of Virology 43:809–818
    [Google Scholar]
  4. Carstens E. B., Tjia S. T., Doerfler W. 1980; Infectious DNA from Autographa californica nuclear polyhedrosis virus. Virology 101:311–314
    [Google Scholar]
  5. Carstens E. B., Krebs A., Gallerneault C. E. 1986; Identification of an amino acid essential to the normal assembly of Autographa californica nuclear polyhedrosis virus polyhedra. Journal of Virology 58:684–688
    [Google Scholar]
  6. Chou P. Y., Fasman G. D. 1978; Prediction of the secondary structure of proteins from their amino acid sequence. Advances in Enzymology 47:45–148
    [Google Scholar]
  7. Cochran M. A., Carstens E. B., Eaton B. T., Faulkner P. 1982; Molecular cloning and physical mapping of restriction endonuclease fragments of Autographa californica nuclear polyhedrosis virus DNA. Journal of Virology 41:940–946
    [Google Scholar]
  8. Duncan R., Faulkner P. 1982; Bromodeoxyuridine-induced mutants of Autographa californica nuclear polyhedrosis virus defective in occlusion body formation. Journal of General Virology 62:369–373
    [Google Scholar]
  9. Duncan R., Chung K. L., Faulkner P. 1982; Analysis of a mutant of Autographa californica nuclear polyhedrosis virus with a defect in the morphogenesis of the occlusion body macromolecular lattice. Journal of General Virology 64:1531–1542
    [Google Scholar]
  10. Gershenson S. M. 1960; A study of a mutant strain of nuclear polyhedral virus of oak silkworm. Problems of Virology English translation of Voprosy virusologii 6:720–725
    [Google Scholar]
  11. Hooft Van Iddekinge B. J. L., Smith G. E., Summers M. D. 1983; Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology 131:561–565
    [Google Scholar]
  12. Iatrou K., Ito K., Witkiewicz H. 1985; Polyhedrin gene of Bombyx mori nuclear polyhedrosis virus. Journal of Virology 54:436–445
    [Google Scholar]
  13. Kozlov E. A., Levitina T. L., Gusak N. M., Serebryani S. B. 1981; Comparison of the amino acid sequence of inclusion body proteins of nuclear polyhedrosis viruses of Bombyx mori, Porthetria dispar and Galleria mellonella . Bioorganicheskaya Khimiya 7:687–696
    [Google Scholar]
  14. Rohel D. Z., Cohran M. A., Faulkner P. 1983; Characterization of two abundant mRNAs of Autographa californica nuclear polyhedrosis virus present late in infection. Virology 124:357–368
    [Google Scholar]
  15. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  16. Smith G. E., Summers M. D. 1980; Restriction map of Rachiplusia ou and Rachiplusia ou-Autographa californica baculovirus recombinants. Journal of Virology 33:311–319
    [Google Scholar]
  17. Smith G. E., Vlak I. M., Summers M. D. 1982; In vitro translation of Autographa californica nuclear polyhedrosis virus early and late mRNA. Journal of Virology 44:199–208
    [Google Scholar]
  18. Stairs G. R. 1964; Selection of a strain of insect granulosis virus producing only cubic inclusion bodies. Virology 24:520–521
    [Google Scholar]
  19. Vaughn J. L., Goodwin R. H., Tompkin G. L., Mccawley P. 1977; The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera:Noctuidae). In Vitro 13:213–217
    [Google Scholar]
  20. Vlak J. M., Rohrmann G. F. 1985; The nature of polyhedrin. In Viral Insecticides for Biological Control pp Maramorosch K., Sherman K. E. Edited by New York: Academic Press;
    [Google Scholar]
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