Protection of Mice Against Dengue 2 Virus Encephalitis by Immunization with the Dengue 2 Virus Non-structural Glycoprotein NS1 Free

Abstract

SUMMARY

Immunization of mice with the dengue 2 virus (DEN 2)-specified non-structural protein NS1 provided significant protection against intracerebral challenge with the virus in the absence of detectable neutralizing or other anti-virion antibody. NS1, purified from lysates of infected Vero cells by immunoaffinity chromatography, expressed an antigenic site(s) common to each of the four DEN serotypes, and hyperimmunization of rabbits with NS1 stimulated production of complement-fixing (CF) antibody with broad DEN serotype specificity. However, cross-protection was not observed: mice immunized with DEN 2 NS1 developed little or no heterologous CF antibody and were not protected against challenge with neurovirulent DEN 1. Induction of a protective immune response by NS1 suggests that it be considered for incorporation into possible synthetic or recombinant DNA DEN vaccines.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-3-853
1987-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/3/JV0680030853.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-3-853&mimeType=html&fmt=ahah

References

  1. Bancroft W. H., Scott R. McN., Eckels K. H., Hoke C. H.Jr Simms T. E., Jesrani K. D. T., Summers P. L., Dubois D. R., Tsoulos D, Russell P. K. 1984; Dengue virus type 2 vaccine: reactogenicity and immunogenicity in soldiers. Journal of Infectious Diseases 149:1005–1010
    [Google Scholar]
  2. Cardiff R. D., Lund J. K. 1976; Distribution of dengue-2 antigens by electron immunocytochemistry. Infection and Immunity 3:1699–1709
    [Google Scholar]
  3. Casey H. L. 1965; Standardized diagnostic complement-fixation method and adaptation to micro test. Public Health Monograph No. 74 U.S. Public Health Service Publication 1228
    [Google Scholar]
  4. Eckels K. H., Scott R. McN., Bancroft W. H., Brown J., Dubois D. R., Summers P. L., Russell P. K., Halstead S. B. 1984; Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. V. Human response to immunization with a candidate vaccine prepared in fet al rhesus lung cells. American Journal of Tropical Medicine and Hygiene 33:684–689
    [Google Scholar]
  5. Gentry M. K., Henchal E. A., McCown J. M., Brandt W. E. 1982; Identification of distinct antigenic determinants on dengue-2 virus by using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 33:548–555
    [Google Scholar]
  6. Halstead S. B. 1980; Dengue haemorrhagic fever - a public health problem and a field for research. Bulletin of the World Health Organization 58:1–21
    [Google Scholar]
  7. Halstead S. B., O’Rourke E. J. 1977; Dengue viruses and mononuclear phagocytes. Journal of Experimental Medicine 146:201–217
    [Google Scholar]
  8. Rice C. M., Lenches E. M., Eddy S. R., Shin S. J., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229:726–733
    [Google Scholar]
  9. Russell P. K., Chiewslip D., Brandt W. E. 1970; Immunoprcipitation analysis of soluble complement-fixing antigens of dengue viruses. Journal of Immunology 105:838–845
    [Google Scholar]
  10. Sabin A. B., Schlesinger R. W. 1945; Production of immunity to dengue with virus modified by propagation in mice. Science 101:640–642
    [Google Scholar]
  11. Schlesinger J. J., Brandriss M. W., Walsh E. E. 1985; Protection against 17D yellow fever encephalitis in mice by passive transfer of monoclonal antibodies to the nonstructural glycoprotein gp48 and by active immunization with gp48. Journal of Immunology 135:2805–2809
    [Google Scholar]
  12. Schlesinger J. J., Brandriss M. W., Cropp C. B., Monath T. P. 1986; Protection against yellow fever in monkeys by immunization with yellow fever virus nonstructural NS1. Journal of Virology 60:1153–1155
    [Google Scholar]
  13. Schlesinger R. W., Gordon I., Frankel J. W., Winter J. W., Patterson P. R., Dorrance W. R. 1956; Clinical and serologic response of man to immunization with attenuated dengue and yellow fever viruses. Journal of Immunology 77:352–364
    [Google Scholar]
  14. Smith G. W., Wright P. J. 1985; Synthesis of proteins and glycoproteins in dengue type 2 virus-infected Vero and Aedes albopictus cells. Journal of General Virology 66:559–571
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-3-853
Loading
/content/journal/jgv/10.1099/0022-1317-68-3-853
Loading

Data & Media loading...

Most cited Most Cited RSS feed