@article{mbs:/content/journal/jgv/10.1099/0022-1317-68-3-825, author = "Nash, A. A. and Jayasuriya, A. and Phelan, J. and Cobbold, S. P. and Waldmann, H. and Prospero, T.", title = "Different Roles for L3T4+ and Lyt 2+ T Cell Subsets in the Control of an Acute Herpes Simplex Virus Infection of the Skin and Nervous System", journal= "Journal of General Virology", year = "1987", volume = "68", number = "3", pages = "825-833", doi = "https://doi.org/10.1099/0022-1317-68-3-825", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-3-825", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "T cell populations", keywords = "monoclonal antibodies", keywords = "HSV", abstract = "Summary Rat monoclonal antibodies were used to deplete selectively Lyt 2 (cytotoxic) and L3T4 (helper) T cell populations in vivo. These antibodies produced >95 % depletion of the respective T cell subset as determined by fluorescent antibody and cytofluoro-graphic analyses. Antibody-treated mice were infected in the ear pinna with herpes simplex virus (HSV) and the induction of virus-specific T cell and antibody responses were monitored during the acute infection. Lyt 2-deficient mice produced delayed hypersensitivity and HSV-specific antibodies comparable to those in untreated animals. However, major histocompatibility complex class I-restricted T cell killing was abolished. In contrast, L3T4-deficient animals failed to produce either primary delayed hypersensitivity response or specific antibodies to the virus, but cytotoxic T cell responses were induced and even augmented in comparison with infected, normal animals. This observation clearly demonstrates that Lyt 2 cytotoxic T cells can be induced in a helper T cell-deficient environment. The ability of T cell subset-deficient mice to clear infectious virus was investigated in the skin of the ear and the part of the nervous system innervating the site of infection. L3T4-deficient animals showed a markedly delayed clearance of virus from the ear and also had a more florid infection of the nervous system. However, Lyt 2-deficient mice cleared the infection in the ear normally, but a severe infection of the nervous system was still observed. The implication of these observations to the pathogenesis of this virus is discussed.", }