1887

Abstract

Summary

Rat monoclonal antibodies were used to deplete selectively Lyt 2 (cytotoxic) and L3T4 (helper) T cell populations . These antibodies produced >95 % depletion of the respective T cell subset as determined by fluorescent antibody and cytofluoro-graphic analyses. Antibody-treated mice were infected in the ear pinna with herpes simplex virus (HSV) and the induction of virus-specific T cell and antibody responses were monitored during the acute infection. Lyt 2-deficient mice produced delayed hypersensitivity and HSV-specific antibodies comparable to those in untreated animals. However, major histocompatibility complex class I-restricted T cell killing was abolished. In contrast, L3T4-deficient animals failed to produce either primary delayed hypersensitivity response or specific antibodies to the virus, but cytotoxic T cell responses were induced and even augmented in comparison with infected, normal animals. This observation clearly demonstrates that Lyt 2 cytotoxic T cells can be induced in a helper T cell-deficient environment. The ability of T cell subset-deficient mice to clear infectious virus was investigated in the skin of the ear and the part of the nervous system innervating the site of infection. L3T4-deficient animals showed a markedly delayed clearance of virus from the ear and also had a more florid infection of the nervous system. However, Lyt 2-deficient mice cleared the infection in the ear normally, but a severe infection of the nervous system was still observed. The implication of these observations to the pathogenesis of this virus is discussed.

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1987-03-01
2022-01-21
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References

  1. Andrus L., Prowse S. J., Lafferty K. J. 1981; Interleukin 2 production by both Ly 2+ and Ly 2- T cell subsets. Scandinavian Journal of Immunology 13:297–301
    [Google Scholar]
  2. Benjamin R. J., Waldmann H. 1986; Induction of tolerance by monoclonal antibody therapy. Nature; London: 320449–451
    [Google Scholar]
  3. Burns W. H., Billups L. C., Notkins A. L. 1975; Thymic dependence of viral antigens. Nature; London: 256654–656
    [Google Scholar]
  4. Cantor H., Boyse E. A. 1975; Functional subclones of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. Journal of Experimental Medicine 141:1390–1399
    [Google Scholar]
  5. Cobbold S. P., Waldmann H. 1986; Skin allograft rejection by L3/T4+ and Lyt2+ T cell subsets. Transplantation 41:634–639
    [Google Scholar]
  6. Cobbold S. P., Jayasuriya A., Nash A., Prospero T. D., Waldmann H. 1984; Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature; London: 312548–551
    [Google Scholar]
  7. Colombatti A., Hilgers J. 1979; A radioimmunoassay for virus antibody using binding of 125I-labelled protein A. Journal of General Virology 43:395–401
    [Google Scholar]
  8. Field H. J., Wildy P. 1978; The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. Journal of Hygiene 81:267–277
    [Google Scholar]
  9. Hill T. J., Field H. J., Blyth W. A. 1975; Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. Journal of General Virology 28:341–353
    [Google Scholar]
  10. Howes E. L., Taylor W., Mitchinson N. A., Simpson E. 1979; MHC matching shows that at least two T cell subsets determine resistance to HSV. Nature; London: 27767–68
    [Google Scholar]
  11. Kapoor A. K., Nash A. A., Wildy P., Phelan J., McLean C. s., Field H. J. 1982; Pathogenesis of herpes simplex virus in congenitally athymic mice: the relative roles of cell-mediated and humoral immunity. Journal of General Virology 60:225–233
    [Google Scholar]
  12. Lanier L. L., Gutman G. A., Lewis D. E., Griswold S. T., Warner N. L. 1982; Monoclonal antibodies against rat immunoglobulin kappa chains. Hybridoma 1:121–131
    [Google Scholar]
  13. Leung K.-N., Nash A. A., Sia D. Y., Wildy P. 1984; Clonal analysis of T cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific T cell clone. Immunology 53:623–633
    [Google Scholar]
  14. Mizuochi T., Golding H., Rosenberg A. S., Glincher L. H., Malek T. R., Singer A. 1985; Both L3T4+ and Lyt 2+ helper T cells initiate cytotoxic T lymphocyte responses against allogeneic major histocompatibility antigens but not against trinitrophenyl modified self. Journal of Experimental Medicine 162:427–443
    [Google Scholar]
  15. Moldwin R. L., Lancki D. W., Herold K. C. 1986; An antigen receptor-driven, interleukin 2- independent pathway for proliferation of murine cytolytic T lymphocyte clones. Journal of Experimental Medicine 163:1566–1582
    [Google Scholar]
  16. Nash A. A., Gell P. G. H. 1983; Membrane phenotype of murine effector and suppressor T cells involved in delayed hypersensitivity and protective immunity to herpes simplex virus. Cellular Immunology 75:348–355
    [Google Scholar]
  17. Nash A. A., Field H. J., Quartey-Parafio R. 1980a; Cell-mediated immunity in herpes simplex virus-infected mice: induction, characterization and antiviral effects of delayed type hypersensitivity. Journal of General Virology 48:351–357
    [Google Scholar]
  18. Nash A. A., Quartey-Papafio R., Wildy P. 1980b; Cell-mediated immunity in herpes simplex virus-infected mice: functional analysis of lymph node cells during periods of acute and latent infection, with reference to cytotoxic and memory cells. Journal of General Virology 49:309–317
    [Google Scholar]
  19. Nash A. A., Phelan J., Wildy P. 1981; Cell-mediated immunity in herpes simplex virus-infected mice: H-2 mapping of the delayed-type hypersensitivity response and the antiviral T cell response. Journal of Immunology 26:1260–1262
    [Google Scholar]
  20. Nash A. A., Leung K-N. 1985; The T-cell-mediated immune response of mice to herpes simplex virus. In The Herpesviruses 4 pp 87–102 Roizman B., Lopez C. Edited by New York: Plenum Press;
    [Google Scholar]
  21. Sprent J., Schaefer M. 1986; Capacity of purified Lyt 2+ T cells to mount primary proliferative and cytotoxic responses to la- tumour cells. Nature; London: 322541–543
    [Google Scholar]
  22. Wagner H., Rollinghoff M. 1978; T-T cell interaction during in vitro cytotoxic allograft responses. I. Soluble products from activated Ly 1+ T cells trigger autonomously antigen-primed Ly 2,3+ T cells to proliferation and cytotoxic activity. Journal of Experimental Medicine 148:1523–1538
    [Google Scholar]
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