%0 Journal Article %A Nikbakht, Kave N. %A Boone, Lawrence R. %A Glover, Paul L. %A Myer, Fred E. %A Yang, Wen K. %T Characterization of a Molecular Clone of RFM/Un Mouse Chromosomal DNA that Contains a Full-length Endogenous Murine Leukaemia Virus-related Proviral Genome %D 1987 %J Journal of General Virology, %V 68 %N 3 %P 683-693 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-68-3-683 %K genome %K provirus %K murine leukaemia virus %I Microbiology Society, %X SUMMARY A 12·4 kbp HindIII chromosomal DNA fragment harbouring an apparently intact 9·2 kbp endogenous murine leukaemia virus (MuLV)-related proviral genome was isolated from an RFM/Un strain mouse by molecular cloning and designated pRFM # 6. Nucleotide sequence analysis revealed the following characteristic features in the pRFM # 6 provirus: a distinct 200 bp sequence in the long terminal repeat (LTR) mid-U3 region, a primer binding site for glutamine tRNA, a 3′ pol region encoding an ‘endonuclease’ protein of 390 amino acids, and the mink cell focusforming virus type-specific sequence at the 5′ portion of the env gene. The 699 bp 5′ LTR and 700 bp 3′ LTR of pRFM # 6 provirus were identical except for three base changes in the U3 ‘enhancer’ region. At the cell-provirus DNA junction, 4 bp direct repeats were present. The proviral genome was found at the same chromosomal DNA site in BALB/c, AKR, C3H, CBA and RFM strain mice, but not in NFS/N or C57BL/6 strain mice. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-3-683