1887

Abstract

SUMMARY

Analysis of the genomic dsRNA of rotaviruses isolated from calves with subclinical infections has revealed eight calves excreting group A viruses with unusual genome profiles. Two of these virus strains, C7–176 and C7–183, were grown and cloned by plaque purification in cell culture. Examination of their genome profiles after cloning showed that the unusual pattern had been retained. They were without RNA segment 11 but had an extra band (6a) migrating between segments 6 and 7. However, they contained the triplet of segments 7, 8 and 9, of similar size, which is characteristic of group A rotaviruses. The number and mol. wt. of the intracellular polypeptides induced by these viruses were similar to those of the bovine group A rotavirus UK strain. Analysis of the RNA transcripts produced by the transcription of purified C7–183 virus showed that segment 6a produced a large RNA transcript of corresponding size. After isolation, this transcript was translated in a rabbit reticulocyte lysate preparation and yielded a single polypeptide, vpll, equivalent to the product of segment 11 of rotaviruses with typical genome profiles.

Keyword(s): bovine , genome profile and rotavirus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-3-653
1987-03-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/3/JV0680030653.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-3-653&mimeType=html&fmt=ahah

References

  1. Albert M. J. 1985; Detection of human rotaviruses with a “super-short” RNA pattern. Acta paediatrica Scandinavia 74:975–976
    [Google Scholar]
  2. Allen A. M., Desselberger u. 1985; Reassortment of human rotaviruses carrying rearranged genomes with bovine rotavirus. Journal of General Virology 66:2703–2714
    [Google Scholar]
  3. Besselaar T. G., Rosenblatt A., Kidd A. H. 1986; Atypical rotavirus from South African neonates. Archives of Virology 87:327–330
    [Google Scholar]
  4. Both G. w., Bellamy A. R., Street J. E., Siegman L. J. 1982; A general strategy for cloning double-stranded RNA: nucleotide sequence of the simian 11 rotavirus gene 8. Nucleic Acids Research 10:7075–7088
    [Google Scholar]
  5. Clarke i. N., Mccrae M. A. 1981; A rapid and sensitive method for analysing the genome profiles of field isolates of rotavirus. Journal of Virological Methods 2:203–209
    [Google Scholar]
  6. Cohen j. 1977; Ribonucleic acid polymerase activity associated with purified calf rotavirus. Journal of General Virology 36:395–402
    [Google Scholar]
  7. Dyall-Smith M. L., Holmes I. H. 1981; Gene-coding assignments of rotavirus double-stranded RNA segments 10 and 11. Journal of Virology 38:1099–1103
    [Google Scholar]
  8. Ericson B. L., Graham D. Y., Mason B. B., Estes M. K. 1982; Identification, synthesis, and modifications of simian rotavirus SA 11 polypeptides in infected cells. Journal of Virology 42:825–839
    [Google Scholar]
  9. Espejo R. T., Munoz o., Serafin F., Romero P. 1980; Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Infection and Immunity 27:351–354
    [Google Scholar]
  10. Flores J., Myslinski J., Kalica A. R., Greenberg H. B., Wyatt R. G., Kapikian A. Z., Chanock R. M. 1982; In vitro transcription of two human rotaviruses. Journal of Virology 43:1032–1037
    [Google Scholar]
  11. Fukusho A., Shimizu Y., Ito Y. 1981; Isolation of cytopathic porcine rotavirus in cell roller culture in the presence of trypsin. Archives of Virology 69:49–60
    [Google Scholar]
  12. Hasegawa A., Inouye S., Matsuno S., Yamaoka K., Eko R., Suharyono W. 1984; Isolation of human rotaviruses with a distinct RNA electrophoretic pattern from Indonesia. Microbiology and Immunology 28:719–722
    [Google Scholar]
  13. Hundley F., Biryahwaho B., Gow M., Desselberger U. 1985; Genome rearrangements of bovine rotavirus after serial passage at high multiplicity of infection. Virology 143:88–103
    [Google Scholar]
  14. Imai M., Richardson M. A., Ikegami N., Shatkin A. J., Furuichi Y. 1983; Molecular cloning OF doublestranded RNA virus genomes. Proceedings of the National Academy of Sciences U.S.A.: 80373–377
    [Google Scholar]
  15. Laemmli u. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  16. Lai M. T., Joklik w. K. 1973; The induction of interferon by temperature-sensitive mutants of reovirus, UV- irradiated reovirus and subvirai reovirus particles. Virology 51:191–201
    [Google Scholar]
  17. Mccrae M. A., Faulkner-Valle G. P. 1981; Molecular biology of rotaviruses. I. Characterisation of basic growth parameters and pattern of macromolecular synthesis. Journal of Virology 39:490–496
    [Google Scholar]
  18. Mccrae M. A., Mccorquodale J. G. 1982a; The molecular biology of rotaviruses. II. Identification of the protein-coding assignments of calf rotavirus genome RNA species. Virology 117:435–443
    [Google Scholar]
  19. Mccrae M. A., Mccorquodale J. G. 1982b; Molecular biology of rotaviruses. IV. Molecular cloning of bovine rotavirus genome. Journal of Virology 44:1076–1079
    [Google Scholar]
  20. Mason B. B., Graham D. Y., Estes M. K. 1980; In vitro transcription and translation of simian rotavirus SA11 gene products. Journal of Virology 33:1111–1121
    [Google Scholar]
  21. Murakami Y., Nishioka N., Hashiguchi Y., Kuniyasu C. 1983; Primary isolation of cytopathic bovine rotaviruses on fetal rhesus monkey kidney cells. Veterinary Microbiology 8:135–139
    [Google Scholar]
  22. Pedley S., Bridger J. C., Brown J. F., Mccrae M. A. 1983; Molecular characterization of rotaviruses with distinct group antigens. Journal of General Virology 64:2093–2101
    [Google Scholar]
  23. Pedley s., Hundley F., Chrystie I., Mccrae M. A., Desselberger U. 1984; The genomes of rotaviruses isolated from chronically infected immunodeficient children. Journal of General Virology 65:1141–1150
    [Google Scholar]
  24. Pedley s., Bridger J. c., Chasey D. 1986; Definition of two new groups of atypical rotaviruses. Journal of General Virology 67:131–137
    [Google Scholar]
  25. Pocock D. H. 1986; Characterisation of bovine rotavirus isolates from sub-clinically infected calves by genome profile analysis. Veterinary Microbiology in press
    [Google Scholar]
  26. Reynolds D. J., Chasey D., Scott A. C., Bridger J. C. 1984; Evaluation of ELISA and electron microscopy for the detection of coronavirus and rotavirus in bovine faeces. Veterinary Record 114:397–401
    [Google Scholar]
  27. Rodger s. M., Holmes I. H. 1979; Comparison of the genomes of simian, bovine and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. Journal of Virology 30:839–846
    [Google Scholar]
  28. Rodger s. M., Bishop R. F., Birch c., Mcclean B. 1981; Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. Journal of Clinical Microbiology 13:272–278
    [Google Scholar]
  29. Thouless M. E., Digiacomo R. F., Neuman D. S. 1986; Isolation of two lapine rotaviruses: characterization of their subgroup, serotype and RNA electropherotypes. Archives of Virology 89:161–170
    [Google Scholar]
  30. Ward C. W., Azad A. A., Dyall-Smith M. L. 1985; Structural homologies between RNA gene segments 10 and 11 from UK bovine, simian SA11, and human Wa rotaviruses. Virology 144:328–336
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-3-653
Loading
/content/journal/jgv/10.1099/0022-1317-68-3-653
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error