1887

Abstract

Summary

A radioimmunoprecipitation assay (RIPA) was used to study the serum antibody responses to individual polypeptides that developed after infection with viruses from human rotavirus subgroups I and II. Paired sera from eight children (1 to 8·5 years of age) were used in the study. Although all of the eight acute sera were negative by the complement fixation test, four of them were positive by RIPA, indicating a previous infection by rotavirus. A significant difference in the number of polypeptides immunoprecipitated was seen among the convalescent sera. The number of polypeptides immunoprecipitated was found to be related to previous infection experience. At most, ten different polypeptides were immunoprecipitated: seven structural polypeptides VP1 to VP7 and three non-structural polypeptides, NS, NS and NS. No sera immunoprecipitated VP8 or VP9. Acute sera positive by RIPA immunoprecipitated up to five polypeptides, VP1, VP2, VP3, VP4 and VP6. One of the non-structural proteins (NS) was found to be particularly immunogenic, since antibodies to this polypeptide were detected in several convalescent sera. Among the structural proteins VP2 and VP6 were found to be the two immunodominant polypeptides which were recognized by all convalescent sera. Only three convalescent sera immunoprecipitated VP7, the major type-specific antigen responsible for inducing neutralizing antibodies. Three of four originally seronegative children with no reactivity in the convalescent sera to VP7 developed neutralizing antibodies to a single serotype. One child developed antibodies to two serotypes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-3-643
1987-03-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/3/JV0680030643.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-3-643&mimeType=html&fmt=ahah

References

  1. Bastardo J. W., Mckimm-Breschkin J. L., Sonza S., Mercer L. D., Holmes I. 1981; Preparation and characterization of antisera to electrophoretically purified SA-11 virus polypeptides. Infection and Immunity 34:641–647
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Clark HF., Dolan K. T., Horton-Slight P. 1985; Diverse serologic response to rotavirus infection of infants in a single epidemic. Pediatric Infectious Diseases 4:626–631
    [Google Scholar]
  4. Ericson B. L., Graham D. Y., Mason B. B. 1982; Identification, synthesis and modifications of simian rotavirus SA-11 polypeptides in infected cells. Journal of Virology 42:825–839
    [Google Scholar]
  5. Estes M. K., Palmer E. L., Obijeski J. F. 1983; Rotaviruses: a review. Current Topics in Microbiology and Immunology 105:123–184
    [Google Scholar]
  6. Greenberg H. B., Valdesuso I., Wyke K., Midthun K., Walsh M., Mcauliffe V., Wyatt R., Kalica A. R., Flores J., Hoshino Y. 1983a; Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. Journal of Virology 47:267–275
    [Google Scholar]
  7. Greenberg H. B., Mcauliffe V., Valdesuso J., Wyatt R., Flores J., Kalica A., Hoshino Y. 1983b; Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infection and Immunity 39:91–99
    [Google Scholar]
  8. Laemmli u. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  9. Mason B. B., Graham D. Y. 1983; Biochemical mapping of the simian rotavirus SA-11 genome. Journal of Virology 46:413–423
    [Google Scholar]
  10. Novo E., Esparza i. 1981; Composition and topography of structural polypeptides of bovine rotavirus. Journal of General Virology 56:325–335
    [Google Scholar]
  11. Offit p. A., Clark HF., Stroop w. G., Twist E. M., Plotkin S. A. 1983a; The cultivation of human rotavirus strain ‘Wa’ to high titer in cell culture and characterization of the viral structural polypeptides. Journal of Virological Methods 7:29–40
    [Google Scholar]
  12. Offit p. A., Clark H. F., Plotkin S. A. 1983b; Response of mice to rotaviruses of bovine or primate origin assessed by radioimmunoassay, radioimmunoprecipitation and plaque reduction neutralization. Infection and Immunity 42:293–300
    [Google Scholar]
  13. Offit P. A., Shaw R. D., Greenberg H. B. 1986; Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins VP3 and VP7. Journal of Virology 58:700–703
    [Google Scholar]
  14. Rodger s. M., Schnagl R. D., Holmes I. H. 1975; Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin. Journal of Virology 16:1229–1235
    [Google Scholar]
  15. Sato K., Inaba Y., Miura Y., Tokuhisa s., Matumoto M. 1982; Antigenic relationships between rotaviruses from different species as studied by neutralization and immunofluorescence. Archives of Virology 73:45–50
    [Google Scholar]
  16. Svensson L. 1984; Identification of an outer capsid glycoprotein of human rotavirus by concanavalin A. Journal of General Virology 65:2183–2190
    [Google Scholar]
  17. Svensson L., Uhnoo I., Grandien M., Wadell G. 1986; Molecular epidemiology of rotavirus infections in Uppsala, Sweden, 1981 : disappearance of a predominant electropherotype. Journal of Medical Virology 18:101–111
    [Google Scholar]
  18. Taniguchi K., Urasawa S., Urasawa T. 1985; Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. Journal of General Virology 66:1045–1053
    [Google Scholar]
  19. Taniguchi K., Urasawa T., Urasawa S. 1986; Reactivity patterns to human rotavirus strains of a monoclonal antibody against VP2, a component of the inner capsid of rotavirus. Archives of Virology 87:135–141
    [Google Scholar]
  20. Uhnoo I., Wadell G., Svensson L., Olding-Stenqvist E., Ekman E., Möllby R. 1986; Aetiology and epidemiology of acute gastroenteritis in Swedish children. Journal of Infection 13:73–89
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-3-643
Loading
/content/journal/jgv/10.1099/0022-1317-68-3-643
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error