@article{mbs:/content/journal/jgv/10.1099/0022-1317-68-2-451, author = "Kennedy, Iain M. and Simpson, Stuart and Macnab, Joan C. M. and Clements, J. Barklie", title = "Human Papillomavirus Type 16 DNA from a Vulvar Carcinoma in situ is present as head-to-Tail Dimeric Episomes with a Deletion in the Non-coding Region", journal= "Journal of General Virology", year = "1987", volume = "68", number = "2", pages = "451-462", doi = "https://doi.org/10.1099/0022-1317-68-2-451", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-2-451", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "human genital cancer", keywords = "genome dimers", keywords = "HPV-16", abstract = "SUMMARY A number of genital cancer biopsy samples were screened for the presence of human papillomavirus type 16 (HPV-16) DNA sequences. One of these samples (a vulvar carcinoma in situ) was found to contain more than 100 copies of HPV-16 DNA sequences per cell. Using this tumour DNA, a genomic library was constructed in bacteriophage lambda and the library was screened for recombinant phage containing HPV-16 sequences. Five recombinant phage clones were isolated and their DNA was analysed by restriction endonuclease digestion and blot hybridization. All five recombinants contained two copies of the HPV-16 genome present in a head-to-tail arrangement. The data are consistent with the presence of HPV-16 sequences in the tumour DNA arranged as genomic dimers in a circular episomal configuration. The HPV-16 genomes contained a deletion within the non-coding region, a region which includes the viral origin of DNA replication and transcriptional control sequences. Possible consequences of this deletion for viral replication and transcription are discussed.", }