1887

Abstract

SUMMARY

Monoclonal antibodies to the envelope glycoproteins, HN and F, of human parainfluenza virus type 3 were coupled to a Sepharose 4B matrix and used for affinity purification of the viral glycoproteins. The purity of the glycoproteins was demonstrated by SDS-PAGE followed by fluorography or silver staining. The antigenicity of the glycoproteins was determined by immunization of rabbits; polyclonal rabbit antisera demonstrated inhibition of functional activities of the virus glycoproteins. The F glycoprotein, when reconstituted into lipid vesicles, showed distinct spike-like projections similar to those of intact virions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-2-409
1987-02-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/2/JV0680020409.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-2-409&mimeType=html&fmt=ahah

References

  1. Bellini W. J., McFarlin D. E., Silver G. D., Mingioli E. S., McFarland H. F. 1981; Immune reactivity of the purified hemagglutinin of measles virus. Infection and Immunity 32:1051–1057
    [Google Scholar]
  2. Chanock R. M., Parott R. H. 1965; Acute respiratory disease in infancy and childhood: present understanding and prospects for prevention. Pediatrics 36:21–39
    [Google Scholar]
  3. Chanock R. M., Bell J. A., Parrott R. H. 1961; Natural history of parainfluenza infection. In Perspectives in Virology 2 pp. 126–130 Pollard M. Edited by Minneapolis: Burgess;
    [Google Scholar]
  4. Choppin P. W., Scheid A. 1980; The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Review of Infectious Diseases 2:40–61
    [Google Scholar]
  5. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  6. Committee On Issues For New Vaccine Development 1985 New Vaccine Development Establishing Priorities 1 pp. 385–396 Division of Health Promotion and Disease Prevention, Institute of Medicine, National Academy of Sciences, U.S.A Washington, D.C: National Academy Press;
    [Google Scholar]
  7. Dean P. D. G., Johnson W. S., Middle F. A. 1985 Affinity Chromatography: A Practical Approach p. 75 Washington, D. C: IRL Press;
    [Google Scholar]
  8. Finberg R., Mischer M., Burakoff S. J. 1978; Induction of virus specific cytotoxic T lymphocytes with isolated membrane proteins. Journal of Experimental Medicine 148:1620–1627
    [Google Scholar]
  9. Glezen W. P., Loda F. A., Clyde W. A., Senior R. J., Shaeffer C. I., Conley W. G., Denny F. W. 1971; Epidemiologic patterns of acute lower respiratory disease in children in a pediatric group practice. Journal of Pediatrics 78:397–406
    [Google Scholar]
  10. Glezen W. P., Frank A. L., Taber L. H., Kasel J. A. 1984; Parainfluenza virus type 3: seasonality and risk of infection and reinfection in young children. Journal of Infectious Diseases 150:851–857
    [Google Scholar]
  11. Goswami K. K. A., Russell W. C. 1983; Monoclonal antibodies against human paramyxovirus type 3 and against SV5 virus: preparation and preliminary characterization. Journal of General Virology 64:1663–1672
    [Google Scholar]
  12. Guskey L. E., Bergtrom G. 1981; High yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins. Journal of General Virology 54:115–123
    [Google Scholar]
  13. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural differences of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 12:1457–1465
    [Google Scholar]
  14. Jambou R. C., Elango N., Venkatesan S. 1985; Proteins associated with human parainfluenza virus type 3. Journal of Virology 56:298–302
    [Google Scholar]
  15. Jennings R., Brand C. M., McLaren C., Shepherd L., Potter C. W. 1974; The immune response of hamsters to purified hemagglutinins and whole influenza virus vaccines following live influenza virus infection. Medical Microbiology and Immunology 160:295–309
    [Google Scholar]
  16. Kasel J. A., Frank A. L., Keitel W. A., Taber L. H., Glezen W. P. 1984; Acquisition of serum antibodies to specific viral glycoproteins of parainfluenza virus 3 in children. Journal of Virology 52:828–832
    [Google Scholar]
  17. Koszinowski V. H., Allen H., Gething M. J., Waterfield M. D., Klenk H. -D. 1980; Recognition of viral glycoproteins by influenza A-specific cross-reactive cytolytic T lymphocytes. Journal of Experimental Medicine 151:945–958
    [Google Scholar]
  18. Loh D., Ross A. H., Hale A. H., Baltimore D., Eisen H. N. 1979; Synthetic phospholipid vesicles containing a purified viral antigen and cell membrane proteins stimulate the development of cytotoxic T lymphocytes. Journal of Experimental Medicine 150:1067–1074
    [Google Scholar]
  19. Merz D. C., Scheid A., Choppin P. W. 1980; Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection. Journal of Experimental Medicine 151:275–288
    [Google Scholar]
  20. Morein B., Helenius A., Simons K., Petterson R., Kaariainen L., Schirrmacher V. 1978; Effective Subunit vaccines against an enveloped animal virus. Nature; London: 276715–718
    [Google Scholar]
  21. Morein B., Sharp M., Sundquist B., Simons K. 1983; Protein subunit vaccines of parainfluenza type 3 virus: immunogenic effect in hamsters and mice. Journal of Experimental Medicine 64:1557–1569
    [Google Scholar]
  22. Mufson M. A., Krause H. E., Mocega H. E., Dawson F. W. 1970; Viruses, Mycoplasma pneumoniae and bacteria associated with respiratory tract disease among infants. American Journal of Epidemiology 91:192–202
    [Google Scholar]
  23. Norrby E., Penttinen K. 1978; Differences in antibodies to the surface components of mumps virus after immunization with formalin inactivated and live virus vaccines. Journal of Infectious Diseases 138:672–676
    [Google Scholar]
  24. Oakley B. R. 1980; A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105:361–363
    [Google Scholar]
  25. Ray R., Compans R. W. 1986; Monoclonal antibodies reveal extensive antigenic differences between the hemagglutinin-neuraminidase glycoproteins of human and bovine parainfluenza 3 viruses. Virology 148:232–236
    [Google Scholar]
  26. Ray R., Brown V. E., Compans R. W. 1985; Glycoproteins of human parainfluenza virus type 3: characterization and evaluation as a subunit vaccine. Journal of Infectious Diseases 152:1219–1230
    [Google Scholar]
  27. Sanchez A., Banerjee A. K. 1985; Studies on human parainfluenza virus 3: characterization of the structural protein and in vitro synthesized proteins coded by mRNAs isolated from infected cells. Virology 143:45–54
    [Google Scholar]
  28. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  29. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. 1972; Isolation of paramyxovirus glycoproteins. Association of both hemagglutinating and neuraminidase activities with the larger SV5 glycoprotein. Virology 50:640–652
    [Google Scholar]
  30. Server A. C., Smith J. A., Waxham M. N., Wolinsky J. S., Goodman H. M. 1985; Purification and amino-terminal protein sequence analysis of the mumps virus fusion protein. Virology 144:373–383
    [Google Scholar]
  31. Storey D. G., Dimock K., Kang C. Y. 1984; Structural characterization of virion proteins and genomic RNA of human parainfluenza virus 3. Journal of Virology 52:761–766
    [Google Scholar]
  32. Tyeryar F. J. JR 1978; Report of a workshop on respiratory syncytial virus and parainfluenza viruses. Journal of Infectious Diseases 137:835–846
    [Google Scholar]
  33. Tyeryar F. J. JR 1983; Report of a workshop on respiratory syncytial virus and parainfluenza viruses. Journal of Infectious Diseases 148:588–598
    [Google Scholar]
  34. Varsanyi T. M., Utter G., Norrby E. 1984; Purification, morphology and antigenic characterization of measles virus envelope components. Journal of General Virology 65:355–366
    [Google Scholar]
  35. Varsanyi T. M., Jornvall H., Norrby E. 1985; Isolation and characterization of the measles virus F1 polypeptide: comparison with other paramyxovirus fusion proteins. Virology 147:110–117
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-2-409
Loading
/content/journal/jgv/10.1099/0022-1317-68-2-409
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error