1887

Abstract

Summary

Monoclonal antibodies to the envelope glycoproteins, HN and F, of human parainfluenza virus type 3 were coupled to a Sepharose 4B matrix and used for affinity purification of the viral glycoproteins. The purity of the glycoproteins was demonstrated by SDS-PAGE followed by fluorography or silver staining. The antigenicity of the glycoproteins was determined by immunization of rabbits; polyclonal rabbit antisera demonstrated inhibition of functional activities of the virus glycoproteins. The F glycoprotein, when reconstituted into lipid vesicles, showed distinct spike-like projections similar to those of intact virions.

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/content/journal/jgv/10.1099/0022-1317-68-2-409
1987-02-01
2019-10-16
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-2-409
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