1887

Abstract

SUMMARY

A biochemical and morphological investigation of the mechanism of entry of vesicular stomatitis virus (VSV) into host cells of mammalian (HeLa), avian (CER), piscine (EPC) and arthropod () origin, is described. VSV was capable of infecting all cell lines tested by a endosome- and/or a lysosome-dependent step since ammonium chloride and amantadine blocked the early stages of infection. Complement-dependent immune lysis of infected host cells provided evidence that in none of the four different cell types examined did insertion of VSV antigens occur from the outside to any great extent on the cell surface. When the entry process was studied by electron microscopy, virus particles were seen to be bound to the cell surface at 0 °C. After warming at 37 °C for homeothermic cells or at 26 °C for poikilothermic cells, virus was detected within coated pits and coated vesicles and, later, in lysosomes. VSV entry was seen to take place by endocytosis in all four cell lines, which were derived from phylogenetically unrelated species.

Keyword(s): endocytosis , entry pathway and VSV
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1987-02-01
2024-03-28
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References

  1. Bussereau F., Chermann J. C., De Clercq E., Hannoun C. 1983; Search for compounds which have an inhibitory effect on rhabdovirus multiplication in vitro. Annates de Virologie 134E:127–134
    [Google Scholar]
  2. Dahlberg J. E. 1974; Quantitative electron microscopic analysis of the penetration of VSV into L cells. Virology 58:250–262
    [Google Scholar]
  3. De Duve C., De Barsy T., Poole B., Tronet A., Tulkens P., Van Hoof F. 1974; Lysosomotropic agents. Biochemical Pharmacology 23:2495–2531
    [Google Scholar]
  4. Fan D. P., Sefton B. M. 1978; The entry into host cells of Sindbis virus, vesicular stomatitis virus and Sendai virus. Cell 15:985–992
    [Google Scholar]
  5. Heine J. W., Schnaitman C. A. 1971; Entry of vesicular stomatitis virus into L cells. Journal of Virology 8:786–795
    [Google Scholar]
  6. Helenius A., Kartenbeck J., Simons K., Fries E. 1980; On the entry of Semliki Forest virus into BHK21 cells. Journal of Cell Biology 84:404–420
    [Google Scholar]
  7. Hors I. 1979; Immunological techniques: determination of HLA antigens by lymphocytotoxicity technique. In NIAID Manual of Tissue Typing Techniques pp. 67–70 Ray J. G.Jr Koff W. C., Knight V. Edited by Bethesda: NIH;
    [Google Scholar]
  8. Koff W. C., Knight V. 1979; Inhibition of influenza virus uncoating by rimantadine hydrochloride. Journal ofVirology 31:261–263
    [Google Scholar]
  9. Lenard J., Miller D. K. 1982; Uncoating of enveloped viruses. Cell 28:5–6
    [Google Scholar]
  10. Mccoombs K., Mann E., Edwards J., Brown D. T. 1981; Effect of chloroquine and cytochalasin B on the infection of cells by Sindbis virus and vesicular stomatitis virus. Journal of Virology 37:1060–1065
    [Google Scholar]
  11. Marsh M., Helenius A. 1980; Adsorptive endocytosis of Semliki Forest virus. Journal of Molecular Biology 142:439–456
    [Google Scholar]
  12. Marsh M., Bolzau E., Helenius A. 1983; Penetration of Semliki Forest virus from acidic prelysosomal vacuoles. Cell 32:931–940
    [Google Scholar]
  13. Matlin K. S., Reggio H., Helenius A., Simons K. 1981; Infectious entry pathway of influenza virus in a canine kidney cell line. Journal of Cell Biology 91:601–613
    [Google Scholar]
  14. Matlin K. S., Reggio H., Simons K., Helenius A. 1982; The pathway of vesicular stomatitis virus entry leading to infection. Journal of Molecular Biology 156:609–631
    [Google Scholar]
  15. Miller D. K., Lenard J. 1981; Antihistamines, local anesthetics and other amines as antiviral agents. Proceedings of the National Academy of Sciences, U.S.A 78:3605–3609
    [Google Scholar]
  16. Ohkuma S., Poole B. 1978; Fluorescence probe measurement of the intralysosomal pH and the perturbation of pH by various agents. Proceedings of the National Academy of Sciences, U.S.A 75:3327–3331
    [Google Scholar]
  17. Oxford J. S., Galbraith A. 1980; Antiviral activity of amantadine: a review on laboratory and clinical data. Pharmacological Therapy 11:181–262
    [Google Scholar]
  18. Reagan K. J., Wunner W. H. 1984; Early interactions of rabies virus with cell surface receptors. In Non-segmentedNegative Strand Viruses pp. 387–392 Bishop D. H. L., Compans R. W. Edited by New York: Academic Press;
    [Google Scholar]
  19. Schlegel R., Willingham M., Pastan I. 1982; Saturable binding sites for vesicular stomatitis virus uptake and receptor-mediated endocytosis of alpha-2-macroglobulin. Proceedings of the National Academy of Sciences, U.S.A 79:2291–2295
    [Google Scholar]
  20. Seganti L., Superti F., Girmenia C., Melucci L., Orsi N. 1986; Study of receptors for vesicular stomatitis virus in vertebrate and invertebrate cells. Microbiologica 9:101–111
    [Google Scholar]
  21. Shimizu Y., Yamamoto S., Homma M., Ishida N. 1972; Effect of chloroquine on the growth of animal viruses. Archiv für die gesamte Virusforschung 36:93–104
    [Google Scholar]
  22. Simpson R. W., Hausen R. E., Dales S. 1969; Viropexis of vesicular stomatitis virus by L cells. Virology 17:285–290
    [Google Scholar]
  23. Superti F., Derer M., Tsiang H. 1984; Mechanism of rabies virus entry pathway into CER cells. Journal of General Virology 65:781–789
    [Google Scholar]
  24. Superti F., Seganti L., Pana A., Orsi N. 1985; Effect of amantadine on rhabdovirus infection. Drugs and Experimental Clinical Research 11:69–74
    [Google Scholar]
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