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A protein of apparent molecular weight 66000 was purified from wheat plants infected with wheat streak mosaic virus. Antiserum to this protein, labelled with gold, specifically stained cylindrical inclusions in ultrathin sections of virus-infected cells. Antiserum to the M r 66000 protein did not react with capsid protein in Western blots, nor did antiserum to capsid protein react with the M r 66000 protein. Both antisera reacted with homologous antigens. The concentration of the M r 66000 protein in extracts from infected leaves was about 100 μg per g of leaves, which is higher than the usual concentration of virions.
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