Abortive Infection by Bacteriophage Me1 of K12 Strains Bearing the Plasmid ColV, I-K94 Free

Abstract

SUMMARY

Bacteriophage Me1 is unable to grow on strains harbouring the ColV,I-K94 plasmid. The nature of this inhibition was investigated, and it was found not to be due to restriction, superinfection exclusion or receptor-mediated resistance, but to be a new example of plasmid-mediated abortive infection. Investigation of events occurring during abortive Me1 infection revealed some differences from previously described cases, especially with regard to late protein synthesis, which did occur, albeit showing abnormal amounts of some proteins. No major differences were observed in membrane permeability of productively and abortively infected cells. Phage-directed DNA synthesis was reduced in abortively infected cells. Comparative studies of Me1 and T4 revealed a striking similarity despite some minor differences.

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1987-02-01
2024-03-29
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References

  1. Binns M. M., Davies D. L., Hardy K. G. 1979; Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance. Nature; London: 279778–781
    [Google Scholar]
  2. Binns M. M., Mayden J., Levine R. P. 1982; Further characterization of complement resistance conferred on Escherichia coli by the plasmid genes traT of R100 and iss of ColV,I-K94. Infection and Immunity 35:654–659
    [Google Scholar]
  3. Brunel F., Davison J., Merchez M. 1979; Cloning of bacteriophage T5 DNA fragments in plasmid pBR322 and bacteriophage λgtWES. Gene 8:53–68
    [Google Scholar]
  4. Clancy J., Savage D. C. 1981; Another colicin V phenotype: in vitro adhesion of Escherichia coli to mouse intestinal epithelium. Infection and Immunity 32:343–352
    [Google Scholar]
  5. Condit R. C. 1975; F-factor mediated inhibition of bacteriophage T7 growth: increased membrane permeability and decreased ATP levels following T7 infection of male Escherichia coli. Journal of Molecular Biology 98:45–56
    [Google Scholar]
  6. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  7. De pacheco C. S., Goodson M., Rossouw F. T., Rowbury R. J. 1985; Effects of the virulence plasmid ColV,I-K94 on the sensitivity of Escherichia coli to putative environmental inhibitory agents. Experientia 41:133–136
    [Google Scholar]
  8. Duckworth D. H., Glenn J., McCorquodale D. J. 1981; Inhibition of bacteriophage replication by extrachromosomal genetic elements. Microbiological Reviews 45:52–71
    [Google Scholar]
  9. Ferroluzzi-ames G., Ames B. N. 1965; The multiplication of T4rII phage in Escherichia coli K12 (λ) in the presence of polyamines. Biochemical and Biophysical Research Communications 18:639–647
    [Google Scholar]
  10. Garen A. 1961; Physiological effects of rII mutants in bacteriophage T4. Virology 14:151–163
    [Google Scholar]
  11. Glenn J., Duckworth D. H. 1979; Amino acid and sugar transport in Escherichia coli (ColIb) during abortive infection by bacteriophage T5. Journal of Virology 30:421–430
    [Google Scholar]
  12. Goldfarb A. 1981; Changes in the promoter range of RNA polymerase resulting from bacteriophage T4 induced modification of core enzyme. Proceedings of the National Academy of Sciences, U.S.A 78:3454–3458
    [Google Scholar]
  13. Jackson R., Cram D., Ray A., Diberardino D., Skurray R. 1984; Cloning and analysis of pif, replication and leading regions of the F-plasmid. Molecular and General Genetics 197:129–136
    [Google Scholar]
  14. Linial M., Malamy M. H. 1970; Studies with bacteriophage ϕII: events following infection of male and female derivatives of Escherichia coli K12. Journal of Virology 5:72–78
    [Google Scholar]
  15. Lugtenberg B., Meijers M., Peters R., Van der hoek P., Van alphen L. 1975; Electrophoretic resolution of the major outer membrane protein of Escherichia coli K12 into four bands. FEBS Letters 58:254–258
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning : A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Meselson M., Yuan R., Heywood J. 1972; Restriction and modification of DNA. Annual Review of Biochemistry 41:447–466
    [Google Scholar]
  18. Moores J. C., Rowbury R. J. 1982; A new major outer membrane protein in derivatives of Escherichia coli carrying the virulence plasmid ColV-K94. Zeitschrift für allgemeine Mikrobiologie 22:465–475
    [Google Scholar]
  19. Morrison T. G., Malamy M. H. 1971; T7 translational control mechanisms and their inhibition by F-factors. Nature; London: 23137–41
    [Google Scholar]
  20. Nemura H., Mizobuchi K. 1982; Inhibition of growth of bacteriophage BF23 by the ColIb plasmid: identification of the ibfA and ibfB genes of the ColIb plasmid. Molecular and General Genetics 185:13–20
    [Google Scholar]
  21. Nilius A. M., Savage D. C. 1984; Serum resistance encoded by colicin V plasmids in Escherichia coli and its relationship to the plasmid transfer system. Infection and Immunity 43:947–953
    [Google Scholar]
  22. Oakley B. R., Kirsch D. R., Morris R. M. 1980; A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105:361–363
    [Google Scholar]
  23. Pinkerton T., Muzyczka N., Walia S., Dunn G., Rose K., Duckworth D. 1981; Bacteriophage T5 growth in Escherichia coli containing cloned PstI fragments of the colicin Ib plasmid. Gene 16:89–96
    [Google Scholar]
  24. Riva S., Cascino A., Geiduschek E. P. 1970a; Coupling of late transcription to viral replication in bacteriophage T4 development. Journal of Molecular Biology 54:85–102
    [Google Scholar]
  25. Riva S., Cascino A., Geiduschek E. P. 1970b; Uncoupling of late transcription from DNA replication in bacteriophage T4 development. Journal of Molecular Biology 54:103–119
    [Google Scholar]
  26. Rowbury R. J., Deeney C. M., Reakes C. F. L., Rossouw F. T., Smith D. G., Tewari R. 1985; Envelope protein changes, autoagglutination, sensitivity to hydrophobic agents and a conditional division lesion in E. coli strains carrying ColV virulence plasmids. Annates de l’Institut Pasteur 136A:147–157
    [Google Scholar]
  27. Schnaitman C., Smith D., Forn de salsas M. 1975; Temperate bacteriophage which causes the production of a new outer membrane protein by Escherichia coli. Journal of Virology 15:1121–1130
    [Google Scholar]
  28. Strobel M., Nomura M. 1966; Restriction of the growth of bacteriophage BF23 by a colicin I (ColI-P9) factor. Virology 28:763–765
    [Google Scholar]
  29. Susskind M. M., Botstein D., Wright A. 1974; Superinfection exclusion by P22 prophage in lysogens of Salmonella typhimurium III: failure of superinfecting phage DNA to enter sieA+ lysogens. Virology 62:350–366
    [Google Scholar]
  30. Verhoef C., De graaf P. J., Lugtenberg E. J. J. 1977; Mapping of a gene for a major outer membrane protein of Escherichia coli K12 with the aid of a newly isolated bacteriophage. Molecular and General Genetics 150:103–105
    [Google Scholar]
  31. Williams smith H. 1974; A search for transmissible pathogenic characters in invasive strains of Escherichia coli: the discovery of a plasmid-controlled toxin and a plasmid-controlled lethal character closely associated, or identical, with colicine V. Journal of General Microbiology 83:95–111
    [Google Scholar]
  32. Williams smith H., Huggins M. B. 1976; Further observations on the association of the colicine V plasmid of Escherichia coli with pathogenicity and with survival in the alimentary tract. Journal of General Microbiology 92:335–350
    [Google Scholar]
  33. Williams smith H., Huggins M. B. 1980; The association of the O18, K1 and H7 antigens and the ColV plasmid of a strain of Escherichia coli with its virulence and immunogenicity. Journal of General Microbiology 121:387–400
    [Google Scholar]
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