1887

Abstract

Summary

The viral capsid protein VP1 of human rhinovirus serotype 2 (HRV2) was cleaved with cyanogen bromide. The peptides thus obtained were separated on an HPLC butyl reversed phase column. Their positions on VP1 were determined by amino-terminal sequencing using the known nucleotide sequence of the genomic RNA of HRV2. The putative carboxy-terminal peptide was further cleaved with trypsin and the resulting fragments were separated on a C18 reversed phase column. Amino-terminal sequencing of the C-terminal peptide revealed alanine as being the carboxy terminus of VP1 in HRV2. This indicates that the processing of the polyprotein is different in HRY2 from the processing previously reported for HRV14 and poliovirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-12-3197
1987-12-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/12/JV0680123197.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-12-3197&mimeType=html&fmt=ahah

References

  1. Agol V. I. 1980; Structure, translation, and replication of picomaviral genomes. Progress in Medical Virologv 26:119–157
    [Google Scholar]
  2. Blaas D., Kuechler E., Vriend G., Arnold E., Luo M., Rossmann M. 1987; Comparison of the three dimensional structure of two human rhinoviruses (HRV2 and HRV14). Proteins in press
    [Google Scholar]
  3. Browne C. A., Bennett H. P. J., Solomon S. 1982; The isolation of peptides by high-performance liquid chromatography using predicted elution positions. Analytical Biochemistry 124:201–208
    [Google Scholar]
  4. Burroughs J. N., Sangar D. V., Clarke B. E., Rowlands D. J., Billiau A., Collen D. 1984; Multiple proteases in foot-and-mouth disease virus replication. Journal of Virology 50:878–883
    [Google Scholar]
  5. Callahan P. L., Mizutani S., Colonno R. J. 1985; Molecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14. Proceedings of the National Academv of Sciences, U.S.A 82:732–736
    [Google Scholar]
  6. Duechler M., Skern T., Sommergruber W., Neubauer CH., Gruendler P., Fogy I., Blaas D., Kuechler E. 1987; Evolutionary relationships within the human rhinovirus genus; comparison of serotypes 89,2 and 14. Proceedings of the National Academy of Sciences, U.S.A 84:2605–2609
    [Google Scholar]
  7. Emini E. A., Elzinga M., Wimmer E. 1982; Carboxy-terminal analysis of poliovirus proteins: termination of poliovirus RNA translation and location of unique poliovirus polyprotein cleavage sites. Journal of Virology 42:194–199
    [Google Scholar]
  8. Hanecak R., Semler B. L., Anderson C. W., Wimmer E. 1982; Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs. Proceedings of the National Academy of Sciences, U.S.A 79:3973–3977
    [Google Scholar]
  9. Hanecak R., Semler B. L., Ariga H., Anderson C. W., Wimmer E. 1984; Expression of a cloned gene segment of poliovirus in E. coli. evidence for autocatalytic production of the viral proteinase. Cell 37:1063–1073
    [Google Scholar]
  10. Iizuka N., Kuge S., Nomoto A. 1987; Complete nucleotide sequence of the genome of coxsackievirus B1. Virology 156:64–73
    [Google Scholar]
  11. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E., Hanecak R., Lee J. J., Van Der Werf S., Anderson C. W., Wimmer E. 1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature; London: 291547–553
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  13. Lindberg A. M., Stålhandske P-O.K., Petterson U. 1987; Genome of coxsackievirus B3. Virology 156:50–63
    [Google Scholar]
  14. Mclean C., Matthews T. J., Rueckert R. R. 1976; Evidence of ambiguous processing and selective degradation in the noncapsid proteins of rhinovirus 1A. Journal of Virology 19:903–914
    [Google Scholar]
  15. Merill C. R., Goldman D., Sedman S. A., Ebert M. H. 1981; Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437–1438
    [Google Scholar]
  16. Nicklin M. J. H., Toyoda H., Murray M. G., Wimmer E. 1986; Proteolytic processing in the replication of polio and related viruses. Biotechnology 4:33–42
    [Google Scholar]
  17. Pallansch M. A., Kew O. M., Semler B. L., Omilianowski D. R., Anderson C. W., Wimmer E., Rueckert R. R. 1984; Protein processing map of poliovirus. Journal of Virology 49:873–880
    [Google Scholar]
  18. Palmenberg A. C., Kirby E. M., Janda M. R., Drake N. L., Duke G. M., Potratz K. F., Collett M. S. 1984; The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral protein coding region. Nucleic Acids Research 12:2969–2985
    [Google Scholar]
  19. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H-J., Johnson J. E., Kamer G., Luo M., Mosser A. G., Rueckert R. R., Sherry B., Vriend G. 1985; Structure of a common cold virus, human rhinovirus 14 (F1RV14). Nature; London: 317145–154
    [Google Scholar]
  20. Rueckert R. R., Wimmer E. 1984; Systematic nomenclature of picornavirus proteins. Journal of Virology 50:957–959
    [Google Scholar]
  21. Semler B. L., Anderson C. W., Kitamura N., Rothberg P. G., Wishart W. L., Wimmer E. 1981; Poliovirus replication proteins: RNA sequence encoding P3-1 b and the sites of proteolytic processing. Proceedings of the National Academy of Sciences, U.S.A 78:3464–3468
    [Google Scholar]
  22. Skern T., Sommergruber W., Blaas D., Gruendler P., Frauendorfer F., Pieler C., Fogy I., Kuechler E. 1985; Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region. Nucleic Acids Research 13:2111–2126
    [Google Scholar]
  23. Smyth D. G. 1967; Techniques in enzymic hydrolysis. Methods in Enzymology 11:214–231
    [Google Scholar]
  24. Stanway G., Hughes P. J., Mountford R. C., Minor P. D., Almond J. W. 1984; The complete nucleotide sequence of a common cold virus: human rhinovirus 14. Nucleic Acids Research 12:7859–7875
    [Google Scholar]
  25. Swank R. T., Munkres K. D. 1971; Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate. Analytical Biochemistry 39:462–477
    [Google Scholar]
  26. Toyoda H., Nicklin M. J. H., Murray M. G., Anderson C. W., Dunn J. J., Studier F. W., Wimmer E. 1986; A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein. Cell 45:761–770
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-12-3197
Loading
/content/journal/jgv/10.1099/0022-1317-68-12-3197
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error