1887

Abstract

SUMMARY

Mouse hybridomas producing antibodies against the structural proteins of strain WV4843, a subgroup B strain of respiratory syncytial (RS) virus, were produced by fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with purified preparations of the virus. After immunoprecipitation tests with [S]methion-ine-labelled extracellular virions, 35 clones found to produce antibodies against the fusion (F) protein, six against the membrane (M) protein, 21 against the nucleocapsid (NP) and eight against the phospho- (P) protein were further characterized. Immunoprecipitation with [H]glucosamine-labelled intracellular virus polypeptides detected nine hybridoma cell lines producing antibodies against the large glyco- (G) protein of the virus. By competitive binding ELISA tests with monoclonal antibodies against each of the structural components, a minimum of two, 24, four, 15 and three epitopes were detected on the G, F, M, NP and P proteins, respectively. Eleven monoclonal antibodies directed against nine epitopes of the F protein could neutralize the infectivity of the virus. In contrast, none of the nine monoclonal antibodies against G could neutralize the infectivity of the virus. In order to find out more about the antigenic relationship between human and bovine RS virus strains all monoclonal antibodies were reacted with subgroup A RS virus and also with three different strains of bovine RS virus and one strain of caprine RS virus in immunofluorescence, ELISA and immunoprecipitation tests. In addition, 31 previously developed monoclonal antibodies against subgroup A virus were reacted with the bovine and caprine strains. The numbers of monoclonal antibodies of subgroup B specific for the B type of the two human subgroups were 9/9, 3/35, 0/6, 0/21, 0/8, for the G, F, M, NP and P proteins, respectively. No antigenic variations were found between the three bovine strains and the caprine strain. They did not react with the nine monoclonal antibodies against the G protein of subgroup B, nor did they react with nine monoclonal antibodies against subgroup A. Most but not all of the monoclonal antibodies against the other structural proteins of the two human RS virus subgroups reacted with the four strains. All 11 monoclonal antibodies against the F protein of subgroup B that could neutralize the infectivity of subgroup B also reacted with the bovine strains and neutralized their infectivity. It is concluded that although the bovine strains share many epitopes with the two human subgroups, they are antigenically distinct from the human viruses.

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1987-12-01
2021-10-20
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References

  1. Anderson L. J., Hierholzer J. C., Tsou C., Hendry R. M., Fernie B. F., Stone Y., McIntosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  2. Coates H. V., Kendrick L., Chanock R. 1963; Antigenic differences between two strains of respiratory syncytial virus. Proceedings of the Society for Experimental Biology and Medicine 112:958–964
    [Google Scholar]
  3. Doggett J. E., Taylor-Robinson D. 1964; Serological studies with respiratory syncytial virus. Archiv fur die gesamte Virusforschung 15:601–608
    [Google Scholar]
  4. Engvall E., Perlmann P. 1972; Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 109:129–135
    [Google Scholar]
  5. Fernie B. f., Gerin J. L. 1982; Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion. Infection and Immunity 37:243–249
    [Google Scholar]
  6. Gimenez H. B., Cash P., Melvin W. t. 1984; Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates. Journal of General Virology 65:963–971
    [Google Scholar]
  7. Gruber C., Levine S. 1985; Respiratory syncytial virus polypeptides. IV. The oligosaccharides of the glycoproteins. Journal of General Virology 66:417–432
    [Google Scholar]
  8. Hendricks D. A., Baradaran K., Mcintosh K., Patterson J. L. 1987; Appearance of a soluble form of the G protein of respiratory syncytial virus in fluids of infected cells. Journal of General Virology 68:1705–1714
    [Google Scholar]
  9. Mcintosh K., Chanock R. M. 1985; Respiratory syncytial virus. In Virology pp 1285–1304 Fields B. N. Edited by New York: Raven;
    [Google Scholar]
  10. Mufson M. A., Örvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  11. Norrby E., Chen S. N., Togashi T., Sheshberadaran H., Johnson K. P. 1982; Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells. Archives of Virology 71:1–11
    [Google Scholar]
  12. Norrby E., Mufson M. A., Sheshberadaran H. 1986; Structural differences between subtype A and B strains of respiratory syncytial virus. Journal of General Virology 67:2721–2729
    [Google Scholar]
  13. Norrby E., Mufson M. A., Alexander H., Houghten R. A., Lerner R. A. 1987; Site directed serology with synthetic peptides representing the large glycoprotein (G) of respiratory syncytial virus. Proceedings of the National Academy of Sciences, U.S.A in press
    [Google Scholar]
  14. Olmsted R. A., Elango N., Prince G. A., Murphy B. R., Johnson P. R., Moss B., Chanock R. M., Collins P. 1986; Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins. Proceedings of the National Academy of Sciences U.S.A.: 837462–7466
    [Google Scholar]
  15. Örvell C. 1978; Structural polypeptides of mumps virus. Journal of General Virology 41:527–539
    [Google Scholar]
  16. Örvell C. 1984; The reactions of monoclonal antibodies with structural proteins of mumps virus. Journal of Immunology 132:2622–2629
    [Google Scholar]
  17. Örvell C., Grandien M. 1982; The effects of monoclonal antibodies on biologic activities of structural proteins of Sendai virus. Journal of Immunology 129:2779–2787
    [Google Scholar]
  18. Örvell C., Norrby E. 1980; Immunological relationships between homologous structural polypeptides of measles and canine distemper virus. Journal of General Virology 50:231–245
    [Google Scholar]
  19. Örvell C., Sheshberadaran H., Norrby E. 1985; Preparation and characterization of monoclonal antibodies directed against four structural components of canine distemper virus. Journal of General Virology 66:443–456
    [Google Scholar]
  20. Satake M., Coligan J. E., Elango N., Norrby E., Venkatesan S. 1985; Respiratory syncytial virus envelope glycoprotein (G) has a novel structure. Nucleic Acids Research 13:7795–7812
    [Google Scholar]
  21. Stott E. J., Taylor G. 1985; Respiratory syncytial virus. Brief review. Archives of Virology 84:1–52
    [Google Scholar]
  22. Taylor G., Stott E. J., Bew M., Fernie B. F., Cote P. J., Collins A. P., Hughes M., Jebbet J. 1984; Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52:137–142
    [Google Scholar]
  23. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1984; Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus. Journal of General Virology 65:761–767
    [Google Scholar]
  24. Walsh E. E., Brandriss M. W., Schlesinger J. J. 1985; Purification and characterization of the respiratory syncytial virus fusion protein. Journal of General Virology 66:409–415
    [Google Scholar]
  25. Ward K. A., Everson J. S., Lambden P. R., Watt P. J. 1984; Antigenic and structural variation in the major nucleocapsid protein of respiratory syncytial virus. Journal of General Virology 65:1749–1757
    [Google Scholar]
  26. Wulff H., Kidd P., Wenner H. A. 1964; Respiratory syncytial virus: observations on antigenic heterogeneity. Proceedings of the Society for Experimental Biology and Medicine 115:240–243
    [Google Scholar]
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