1887

Abstract

SUMMARY

The LI and L2 capsid proteins encoded by human papillomavirus types 6 and 16 (HPV-6 and HPV-16) have been synthesized in bacteria. Antisera were raised against the HPV-6 LI- and L2--galactosidase fusion proteins and against an HPV-16 LI C-terminal peptide which was 14 amino acids long. The HPV-16 LI peptide antibodies have been shown to be highly reactive with the HPV-16 Ll--galactosidase fusion protein but not against the equivalent HPV-6 L1 --galactosidase fusion protein. The effectiveness of these antibodies was compared with commercially available antibovine papillomavirus type 1 (BPV-1) antibodies and the results demonstrated that the anti-BPV-1 antibodies reacted well against HPV-6 L1 --galactosidase but not against HPV-16 Ll--galactosidase. In addition, the L2 portion of the HPV-6 L2- -galactosidase fusion protein appeared particularly immunogenic, since antibodies raised against this fusion protein were predominantly reactive with the L2 moiety. The HPV-16 LI peptide antibodies described here will be preferred reagents for the specific detection of HPV-16 capsid antigens, which may be particularly important in early diagnosis of HPV-16 infection.

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/content/journal/jgv/10.1099/0022-1317-68-12-3081
1987-12-01
2021-10-19
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